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SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus

BACKGROUND: The COVID-19 pandemic has highlighted the need for rapid, cost effective and easy-to-use diagnostic tools for SARS-CoV-2 infections that can be used in point of care settings to limit disease transmission. OBJECTIVE: We evaluated two rapid antigen immunochromatographic tests, Abbott Panb...

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Autores principales: Nordgren, Johan, Sharma, Sumit, Olsson, Henrik, Jämtberg, Mikael, Falkeborn, Tina, Svensson, Lennart, Hagbom, Marie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Published by Elsevier B.V. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105081/
https://www.ncbi.nlm.nih.gov/pubmed/33971580
http://dx.doi.org/10.1016/j.jcv.2021.104846
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author Nordgren, Johan
Sharma, Sumit
Olsson, Henrik
Jämtberg, Mikael
Falkeborn, Tina
Svensson, Lennart
Hagbom, Marie
author_facet Nordgren, Johan
Sharma, Sumit
Olsson, Henrik
Jämtberg, Mikael
Falkeborn, Tina
Svensson, Lennart
Hagbom, Marie
author_sort Nordgren, Johan
collection PubMed
description BACKGROUND: The COVID-19 pandemic has highlighted the need for rapid, cost effective and easy-to-use diagnostic tools for SARS-CoV-2 infections that can be used in point of care settings to limit disease transmission. OBJECTIVE: We evaluated two rapid antigen immunochromatographic tests, Abbott Panbio™ COVID-19 Ag Rapid Test (Panbio) and Zhejiang Orient Gene/Healgen Biotech Coronavirus Ag rapid test cassette (Orient gene) for detection of infectious SARS-CoV-2. RESULTS: The tests were evaluated on nasopharyngeal samples taken from individuals having respiratory and/or COVID-19 related symptoms, which had been analyzed for SARS-CoV-2 RNA using real-time PCR. In total 156 PCR-positive, and 130 (Panbio) and 176 (Orient Gene) PCR-negative samples were analyzed. Overall sensitivity and specificity were 71.8% and 100% for Panbio and 79.5% and 74.4% for the Orient Gene test respectively. The false positives by the Orient Gene test were verified as SARS-CoV-2 negative by in-house real-time PCR assay and were negative for the four seasonal coronaviruses. Subgroup analysis revealed that the antigen tests had high sensitivity for samples with Ct-values <25 (>88%) and for samples containing infectious viruses as determined by cultivation on Vero cells, 94.1% and 97.1% for the Panbio and Orient gene tests, respectively. Furthermore, both tests had a sensitivity of <50 picogram for nucleocapsid protein. No sample with a Ct-value >27 was shown to contain infectious virus. CONCLUSION: The results indicate that the rapid antigen tests, especially the Panbio tests may be a valuable tool to detect contagious persons during the ongoing pandemic.
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spelling pubmed-81050812021-05-10 SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus Nordgren, Johan Sharma, Sumit Olsson, Henrik Jämtberg, Mikael Falkeborn, Tina Svensson, Lennart Hagbom, Marie J Clin Virol Article BACKGROUND: The COVID-19 pandemic has highlighted the need for rapid, cost effective and easy-to-use diagnostic tools for SARS-CoV-2 infections that can be used in point of care settings to limit disease transmission. OBJECTIVE: We evaluated two rapid antigen immunochromatographic tests, Abbott Panbio™ COVID-19 Ag Rapid Test (Panbio) and Zhejiang Orient Gene/Healgen Biotech Coronavirus Ag rapid test cassette (Orient gene) for detection of infectious SARS-CoV-2. RESULTS: The tests were evaluated on nasopharyngeal samples taken from individuals having respiratory and/or COVID-19 related symptoms, which had been analyzed for SARS-CoV-2 RNA using real-time PCR. In total 156 PCR-positive, and 130 (Panbio) and 176 (Orient Gene) PCR-negative samples were analyzed. Overall sensitivity and specificity were 71.8% and 100% for Panbio and 79.5% and 74.4% for the Orient Gene test respectively. The false positives by the Orient Gene test were verified as SARS-CoV-2 negative by in-house real-time PCR assay and were negative for the four seasonal coronaviruses. Subgroup analysis revealed that the antigen tests had high sensitivity for samples with Ct-values <25 (>88%) and for samples containing infectious viruses as determined by cultivation on Vero cells, 94.1% and 97.1% for the Panbio and Orient gene tests, respectively. Furthermore, both tests had a sensitivity of <50 picogram for nucleocapsid protein. No sample with a Ct-value >27 was shown to contain infectious virus. CONCLUSION: The results indicate that the rapid antigen tests, especially the Panbio tests may be a valuable tool to detect contagious persons during the ongoing pandemic. Published by Elsevier B.V. 2021-07 2021-04-24 /pmc/articles/PMC8105081/ /pubmed/33971580 http://dx.doi.org/10.1016/j.jcv.2021.104846 Text en © 2021 Published by Elsevier B.V. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Nordgren, Johan
Sharma, Sumit
Olsson, Henrik
Jämtberg, Mikael
Falkeborn, Tina
Svensson, Lennart
Hagbom, Marie
SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus
title SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus
title_full SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus
title_fullStr SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus
title_full_unstemmed SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus
title_short SARS-CoV-2 rapid antigen test: High sensitivity to detect infectious virus
title_sort sars-cov-2 rapid antigen test: high sensitivity to detect infectious virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105081/
https://www.ncbi.nlm.nih.gov/pubmed/33971580
http://dx.doi.org/10.1016/j.jcv.2021.104846
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