A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination

Vaccination is crucial in combatting the severe acute respiratory syndrome coronavirus 2 pandemic. The rare complication of thrombocytopenia and thrombotic complications at unusual sites after ChAdOx1 nCov-19 vaccination is caused by platelet-activating antibodies directed against platelet factor 4...

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Autores principales: Handtke, Stefan, Wolff, Martina, Zaninetti, Carlo, Wesche, Jan, Schönborn, Linda, Aurich, Konstanze, Ulm, Lena, Hübner, Nils-Olaf, Becker, Karsten, Thiele, Thomas, Greinacher, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Hematology 2021
Materias:
Thrombosis and Hemostasis
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105122/
https://www.ncbi.nlm.nih.gov/pubmed/33945605
http://dx.doi.org/10.1182/blood.2021012064
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author Handtke, Stefan
Wolff, Martina
Zaninetti, Carlo
Wesche, Jan
Schönborn, Linda
Aurich, Konstanze
Ulm, Lena
Hübner, Nils-Olaf
Becker, Karsten
Thiele, Thomas
Greinacher, Andreas
author_facet Handtke, Stefan
Wolff, Martina
Zaninetti, Carlo
Wesche, Jan
Schönborn, Linda
Aurich, Konstanze
Ulm, Lena
Hübner, Nils-Olaf
Becker, Karsten
Thiele, Thomas
Greinacher, Andreas
author_sort Handtke, Stefan
collection PubMed
description Vaccination is crucial in combatting the severe acute respiratory syndrome coronavirus 2 pandemic. The rare complication of thrombocytopenia and thrombotic complications at unusual sites after ChAdOx1 nCov-19 vaccination is caused by platelet-activating antibodies directed against platelet factor 4 (PF4). We present a widely applicable whole-blood standard flow cytometric assay to identify the pathogenic antibodies associated with vaccine-induced immune-mediated thrombotic thrombocytopenia (VITT) after ChAdOx1 nCov-19 vaccination. This assay will enable rapid diagnosis by many laboratories. This trial was registered at www.clinicaltrials.gov as #NCT04370119.
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spelling pubmed-81051222021-05-10 A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination Handtke, Stefan Wolff, Martina Zaninetti, Carlo Wesche, Jan Schönborn, Linda Aurich, Konstanze Ulm, Lena Hübner, Nils-Olaf Becker, Karsten Thiele, Thomas Greinacher, Andreas Blood Thrombosis and Hemostasis Vaccination is crucial in combatting the severe acute respiratory syndrome coronavirus 2 pandemic. The rare complication of thrombocytopenia and thrombotic complications at unusual sites after ChAdOx1 nCov-19 vaccination is caused by platelet-activating antibodies directed against platelet factor 4 (PF4). We present a widely applicable whole-blood standard flow cytometric assay to identify the pathogenic antibodies associated with vaccine-induced immune-mediated thrombotic thrombocytopenia (VITT) after ChAdOx1 nCov-19 vaccination. This assay will enable rapid diagnosis by many laboratories. This trial was registered at www.clinicaltrials.gov as #NCT04370119. American Society of Hematology 2021-07-01 /pmc/articles/PMC8105122/ /pubmed/33945605 http://dx.doi.org/10.1182/blood.2021012064 Text en © 2021 by The American Society of Hematology This article is made available via the PMC Open Access Subset for unrestricted reuse and analyses in any form or by any means with acknowledgment of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.
spellingShingle Thrombosis and Hemostasis
Handtke, Stefan
Wolff, Martina
Zaninetti, Carlo
Wesche, Jan
Schönborn, Linda
Aurich, Konstanze
Ulm, Lena
Hübner, Nils-Olaf
Becker, Karsten
Thiele, Thomas
Greinacher, Andreas
A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination
title A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination
title_full A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination
title_fullStr A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination
title_full_unstemmed A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination
title_short A flow cytometric assay to detect platelet-activating antibodies in VITT after ChAdOx1 nCov-19 vaccination
title_sort flow cytometric assay to detect platelet-activating antibodies in vitt after chadox1 ncov-19 vaccination
topic Thrombosis and Hemostasis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105122/
https://www.ncbi.nlm.nih.gov/pubmed/33945605
http://dx.doi.org/10.1182/blood.2021012064
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