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Novel RT-ddPCR assays for measuring the levels of subgenomic and genomic SARS-CoV-2 transcripts

The replication of SARS-CoV-2 and other coronaviruses depends on transcription of negative-sense RNA intermediates that serve as the templates for the synthesis of positive-sense genomic RNA (gRNA) and multiple different subgenomic mRNAs (sgRNAs) encompassing fragments arising from discontinuous tra...

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Detalles Bibliográficos
Autores principales:	Telwatte, Sushama, Martin, Holly Anne, Marczak, Ryan, Fozouni, Parinaz, Vallejo-Gracia, Albert, Kumar, G. Renuka, Murray, Victoria, Lee, Sulggi, Ott, Melanie, Wong, Joseph K., Yukl, Steven A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Academic Press 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105137/ https://www.ncbi.nlm.nih.gov/pubmed/33882362 http://dx.doi.org/10.1016/j.ymeth.2021.04.011

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