A chimeric dengue virus vaccine candidate delivered by high density microarray patches protects against infection in mice

Dengue viruses (DENV) cause an estimated 390 million infections globally. With no dengue-specific therapeutic treatment currently available, vaccination is the most promising strategy for its control. A wide range of DENV vaccines are in development, with one having already been licensed, albeit wit...

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Autores principales: Choo, Jovin J. Y., Vet, Laura J., McMillan, Christopher L. D., Harrison, Jessica J., Scott, Connor A. P., Depelsenaire, Alexandra C. I., Fernando, Germain J. P., Watterson, Daniel, Hall, Roy A., Young, Paul R., Hobson-Peters, Jody, Muller, David A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105366/
https://www.ncbi.nlm.nih.gov/pubmed/33963191
http://dx.doi.org/10.1038/s41541-021-00328-1
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author Choo, Jovin J. Y.
Vet, Laura J.
McMillan, Christopher L. D.
Harrison, Jessica J.
Scott, Connor A. P.
Depelsenaire, Alexandra C. I.
Fernando, Germain J. P.
Watterson, Daniel
Hall, Roy A.
Young, Paul R.
Hobson-Peters, Jody
Muller, David A.
author_facet Choo, Jovin J. Y.
Vet, Laura J.
McMillan, Christopher L. D.
Harrison, Jessica J.
Scott, Connor A. P.
Depelsenaire, Alexandra C. I.
Fernando, Germain J. P.
Watterson, Daniel
Hall, Roy A.
Young, Paul R.
Hobson-Peters, Jody
Muller, David A.
author_sort Choo, Jovin J. Y.
collection PubMed
description Dengue viruses (DENV) cause an estimated 390 million infections globally. With no dengue-specific therapeutic treatment currently available, vaccination is the most promising strategy for its control. A wide range of DENV vaccines are in development, with one having already been licensed, albeit with limited distribution. We investigated the immunogenicity and protective efficacy of a chimeric virus vaccine candidate based on the insect-specific flavivirus, Binjari virus (BinJV), displaying the structural prM/E proteins of DENV (BinJ/DENV2-prME). In this study, we immunized AG129 mice with BinJ/DENV2-prME via a needle-free, high-density microarray patch (HD-MAP) delivery system. Immunization with a single, 1 µg dose of BinJ/DENV2-prME delivered via the HD-MAPs resulted in enhanced kinetics of neutralizing antibody induction when compared to needle delivery and complete protection against mortality upon virus challenge in the AG129 DENV mouse model.
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spelling pubmed-81053662021-05-11 A chimeric dengue virus vaccine candidate delivered by high density microarray patches protects against infection in mice Choo, Jovin J. Y. Vet, Laura J. McMillan, Christopher L. D. Harrison, Jessica J. Scott, Connor A. P. Depelsenaire, Alexandra C. I. Fernando, Germain J. P. Watterson, Daniel Hall, Roy A. Young, Paul R. Hobson-Peters, Jody Muller, David A. NPJ Vaccines Article Dengue viruses (DENV) cause an estimated 390 million infections globally. With no dengue-specific therapeutic treatment currently available, vaccination is the most promising strategy for its control. A wide range of DENV vaccines are in development, with one having already been licensed, albeit with limited distribution. We investigated the immunogenicity and protective efficacy of a chimeric virus vaccine candidate based on the insect-specific flavivirus, Binjari virus (BinJV), displaying the structural prM/E proteins of DENV (BinJ/DENV2-prME). In this study, we immunized AG129 mice with BinJ/DENV2-prME via a needle-free, high-density microarray patch (HD-MAP) delivery system. Immunization with a single, 1 µg dose of BinJ/DENV2-prME delivered via the HD-MAPs resulted in enhanced kinetics of neutralizing antibody induction when compared to needle delivery and complete protection against mortality upon virus challenge in the AG129 DENV mouse model. Nature Publishing Group UK 2021-05-07 /pmc/articles/PMC8105366/ /pubmed/33963191 http://dx.doi.org/10.1038/s41541-021-00328-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Choo, Jovin J. Y.
Vet, Laura J.
McMillan, Christopher L. D.
Harrison, Jessica J.
Scott, Connor A. P.
Depelsenaire, Alexandra C. I.
Fernando, Germain J. P.
Watterson, Daniel
Hall, Roy A.
Young, Paul R.
Hobson-Peters, Jody
Muller, David A.
A chimeric dengue virus vaccine candidate delivered by high density microarray patches protects against infection in mice
title A chimeric dengue virus vaccine candidate delivered by high density microarray patches protects against infection in mice
title_full A chimeric dengue virus vaccine candidate delivered by high density microarray patches protects against infection in mice
title_fullStr A chimeric dengue virus vaccine candidate delivered by high density microarray patches protects against infection in mice
title_full_unstemmed A chimeric dengue virus vaccine candidate delivered by high density microarray patches protects against infection in mice
title_short A chimeric dengue virus vaccine candidate delivered by high density microarray patches protects against infection in mice
title_sort chimeric dengue virus vaccine candidate delivered by high density microarray patches protects against infection in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105366/
https://www.ncbi.nlm.nih.gov/pubmed/33963191
http://dx.doi.org/10.1038/s41541-021-00328-1
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