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A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasmapneumoniae based on MALDI-TOF MS
In this study, a multisite SNP genotyping and macrolide (ML) susceptibility gene test method for Mycoplasma pneumoniae (M. pneumoniae) was developed based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The detection limit of this method for nucleic ac...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105657/ https://www.ncbi.nlm.nih.gov/pubmed/33997713 http://dx.doi.org/10.1016/j.isci.2021.102447 |
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author | Zhao, Fei Zhang, Jianzhong Wang, Xuemei Liu, Liyong Gong, Jie Zhai, Zhixiang He, Lihua Meng, Fanliang Xiao, Di |
author_facet | Zhao, Fei Zhang, Jianzhong Wang, Xuemei Liu, Liyong Gong, Jie Zhai, Zhixiang He, Lihua Meng, Fanliang Xiao, Di |
author_sort | Zhao, Fei |
collection | PubMed |
description | In this study, a multisite SNP genotyping and macrolide (ML) susceptibility gene test method for Mycoplasma pneumoniae (M. pneumoniae) was developed based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The detection limit of this method for nucleic acids was 10(2) -10(3) copies/reaction. Six SNP site-based genotyping and 3 ML susceptibility sites could be detected simultaneously based on multiplex PCR and mass probe. Using the method constructed in this study, 141 Chinese clinical isolates were divided into 8 SNP types. All the SNP test results for the ML susceptibility gene were in line with those of the 23S rRNA sequencing results. With this method, the multisite SNP genotyping and ML susceptibility determination of M. pneumoniae can be completed simultaneously in one test, which greatly reduces the workload and cost, improves the genotyping ability of M. pneumoniae and deserves clinical application. |
format | Online Article Text |
id | pubmed-8105657 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-81056572021-05-14 A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasmapneumoniae based on MALDI-TOF MS Zhao, Fei Zhang, Jianzhong Wang, Xuemei Liu, Liyong Gong, Jie Zhai, Zhixiang He, Lihua Meng, Fanliang Xiao, Di iScience Article In this study, a multisite SNP genotyping and macrolide (ML) susceptibility gene test method for Mycoplasma pneumoniae (M. pneumoniae) was developed based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The detection limit of this method for nucleic acids was 10(2) -10(3) copies/reaction. Six SNP site-based genotyping and 3 ML susceptibility sites could be detected simultaneously based on multiplex PCR and mass probe. Using the method constructed in this study, 141 Chinese clinical isolates were divided into 8 SNP types. All the SNP test results for the ML susceptibility gene were in line with those of the 23S rRNA sequencing results. With this method, the multisite SNP genotyping and ML susceptibility determination of M. pneumoniae can be completed simultaneously in one test, which greatly reduces the workload and cost, improves the genotyping ability of M. pneumoniae and deserves clinical application. Elsevier 2021-04-16 /pmc/articles/PMC8105657/ /pubmed/33997713 http://dx.doi.org/10.1016/j.isci.2021.102447 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Zhao, Fei Zhang, Jianzhong Wang, Xuemei Liu, Liyong Gong, Jie Zhai, Zhixiang He, Lihua Meng, Fanliang Xiao, Di A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasmapneumoniae based on MALDI-TOF MS |
title | A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasmapneumoniae based on MALDI-TOF MS |
title_full | A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasmapneumoniae based on MALDI-TOF MS |
title_fullStr | A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasmapneumoniae based on MALDI-TOF MS |
title_full_unstemmed | A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasmapneumoniae based on MALDI-TOF MS |
title_short | A multisite SNP genotyping and macrolide susceptibility gene method for Mycoplasmapneumoniae based on MALDI-TOF MS |
title_sort | multisite snp genotyping and macrolide susceptibility gene method for mycoplasmapneumoniae based on maldi-tof ms |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105657/ https://www.ncbi.nlm.nih.gov/pubmed/33997713 http://dx.doi.org/10.1016/j.isci.2021.102447 |
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