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Elimination of nurse cell nuclei that shuttle into oocytes during oogenesis

Drosophila oocytes develop together with 15 sister germline nurse cells (NCs), which pass products to the oocyte through intercellular bridges. The NCs are completely eliminated during stages 12–14, but we discovered that at stage 10B, two specific NCs fuse with the oocyte and extrude their nuclei t...

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Autores principales: Ali-Murthy, Zehra, Fetter, Richard D., Wang, Wanpeng, Yang, Bin, Royer, Loic A., Kornberg, Thomas B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105724/
https://www.ncbi.nlm.nih.gov/pubmed/33950159
http://dx.doi.org/10.1083/jcb.202012101
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author Ali-Murthy, Zehra
Fetter, Richard D.
Wang, Wanpeng
Yang, Bin
Royer, Loic A.
Kornberg, Thomas B.
author_facet Ali-Murthy, Zehra
Fetter, Richard D.
Wang, Wanpeng
Yang, Bin
Royer, Loic A.
Kornberg, Thomas B.
author_sort Ali-Murthy, Zehra
collection PubMed
description Drosophila oocytes develop together with 15 sister germline nurse cells (NCs), which pass products to the oocyte through intercellular bridges. The NCs are completely eliminated during stages 12–14, but we discovered that at stage 10B, two specific NCs fuse with the oocyte and extrude their nuclei through a channel that opens in the anterior face of the oocyte. These nuclei extinguish in the ooplasm, leaving 2 enucleated and 13 nucleated NCs. At stage 11, the cell boundaries of the oocyte are mostly restored. Oocytes in egg chambers that fail to eliminate NC nuclei at stage 10B develop with abnormal morphology. These findings show that stage 10B NCs are distinguished by position and identity, and that NC elimination proceeds in two stages: first at stage 10B and later at stages 12–14.
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spelling pubmed-81057242022-01-05 Elimination of nurse cell nuclei that shuttle into oocytes during oogenesis Ali-Murthy, Zehra Fetter, Richard D. Wang, Wanpeng Yang, Bin Royer, Loic A. Kornberg, Thomas B. J Cell Biol Article Drosophila oocytes develop together with 15 sister germline nurse cells (NCs), which pass products to the oocyte through intercellular bridges. The NCs are completely eliminated during stages 12–14, but we discovered that at stage 10B, two specific NCs fuse with the oocyte and extrude their nuclei through a channel that opens in the anterior face of the oocyte. These nuclei extinguish in the ooplasm, leaving 2 enucleated and 13 nucleated NCs. At stage 11, the cell boundaries of the oocyte are mostly restored. Oocytes in egg chambers that fail to eliminate NC nuclei at stage 10B develop with abnormal morphology. These findings show that stage 10B NCs are distinguished by position and identity, and that NC elimination proceeds in two stages: first at stage 10B and later at stages 12–14. Rockefeller University Press 2021-05-05 /pmc/articles/PMC8105724/ /pubmed/33950159 http://dx.doi.org/10.1083/jcb.202012101 Text en © 2021 Ali-Murthy et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Ali-Murthy, Zehra
Fetter, Richard D.
Wang, Wanpeng
Yang, Bin
Royer, Loic A.
Kornberg, Thomas B.
Elimination of nurse cell nuclei that shuttle into oocytes during oogenesis
title Elimination of nurse cell nuclei that shuttle into oocytes during oogenesis
title_full Elimination of nurse cell nuclei that shuttle into oocytes during oogenesis
title_fullStr Elimination of nurse cell nuclei that shuttle into oocytes during oogenesis
title_full_unstemmed Elimination of nurse cell nuclei that shuttle into oocytes during oogenesis
title_short Elimination of nurse cell nuclei that shuttle into oocytes during oogenesis
title_sort elimination of nurse cell nuclei that shuttle into oocytes during oogenesis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8105724/
https://www.ncbi.nlm.nih.gov/pubmed/33950159
http://dx.doi.org/10.1083/jcb.202012101
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