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Electrospun fibrous sponge via short fiber for mimicking 3D ECM

BACKGROUND: Most of the natural extracellular matrix (ECM) is a three-dimensional (3D) network structure of micro/nanofibers for cell adhesion and growth of 3D. Electrospun fibers distinctive mimicked 2D ECM, however, it is impossible to simulate 3D ECM because of longitudinal collapse of continuous...

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Autores principales: Li, Yan, Wang, Juan, Qian, Dejian, Chen, Liang, Mo, Xiumei, Wang, Lei, Wang, Yan, Cui, Wenguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106196/
https://www.ncbi.nlm.nih.gov/pubmed/33964948
http://dx.doi.org/10.1186/s12951-021-00878-5
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author Li, Yan
Wang, Juan
Qian, Dejian
Chen, Liang
Mo, Xiumei
Wang, Lei
Wang, Yan
Cui, Wenguo
author_facet Li, Yan
Wang, Juan
Qian, Dejian
Chen, Liang
Mo, Xiumei
Wang, Lei
Wang, Yan
Cui, Wenguo
author_sort Li, Yan
collection PubMed
description BACKGROUND: Most of the natural extracellular matrix (ECM) is a three-dimensional (3D) network structure of micro/nanofibers for cell adhesion and growth of 3D. Electrospun fibers distinctive mimicked 2D ECM, however, it is impossible to simulate 3D ECM because of longitudinal collapse of continuous micro/nanofibers. Herein, 3D electrospun micro/nano-fibrous sponge was fabricated via electrospinning, homogenization, shaping and thermal crosslinking for 3D tissue regeneration of cells and vascular. RESULTS: Fibrous sponge exhibited high porosity, water absorption and compression resilience and no chemical crosslinked agent was used in preparation process. In vitro studies showed that the 3D short fiber sponge provided an oxygen-rich environment for cell growth, which was conducive to the 3D proliferation and growth of HUVECs, stimulated the expression of VEGF, and well promoted the vascularization of HUVECs. In vivo studies showed that the 3D short fiber sponges had a good 3D adhesion to the chronic wound of diabetes in rats. Furthermore, 3D short fibrous sponges were better than 2D micro/nanofiber membranes in promoting the repair of diabetic full-thickness skin defects including wound healing, hair follicle regeneration, angiogenesis, collagen secretion. CONCLUSION: Therefore, electrospun short fibrous sponges are special candidates for mimicking the 3D ECM and promoting 3D regeneration of tissue. GRAPHIC ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-021-00878-5.
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spelling pubmed-81061962021-05-10 Electrospun fibrous sponge via short fiber for mimicking 3D ECM Li, Yan Wang, Juan Qian, Dejian Chen, Liang Mo, Xiumei Wang, Lei Wang, Yan Cui, Wenguo J Nanobiotechnology Research BACKGROUND: Most of the natural extracellular matrix (ECM) is a three-dimensional (3D) network structure of micro/nanofibers for cell adhesion and growth of 3D. Electrospun fibers distinctive mimicked 2D ECM, however, it is impossible to simulate 3D ECM because of longitudinal collapse of continuous micro/nanofibers. Herein, 3D electrospun micro/nano-fibrous sponge was fabricated via electrospinning, homogenization, shaping and thermal crosslinking for 3D tissue regeneration of cells and vascular. RESULTS: Fibrous sponge exhibited high porosity, water absorption and compression resilience and no chemical crosslinked agent was used in preparation process. In vitro studies showed that the 3D short fiber sponge provided an oxygen-rich environment for cell growth, which was conducive to the 3D proliferation and growth of HUVECs, stimulated the expression of VEGF, and well promoted the vascularization of HUVECs. In vivo studies showed that the 3D short fiber sponges had a good 3D adhesion to the chronic wound of diabetes in rats. Furthermore, 3D short fibrous sponges were better than 2D micro/nanofiber membranes in promoting the repair of diabetic full-thickness skin defects including wound healing, hair follicle regeneration, angiogenesis, collagen secretion. CONCLUSION: Therefore, electrospun short fibrous sponges are special candidates for mimicking the 3D ECM and promoting 3D regeneration of tissue. GRAPHIC ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-021-00878-5. BioMed Central 2021-05-08 /pmc/articles/PMC8106196/ /pubmed/33964948 http://dx.doi.org/10.1186/s12951-021-00878-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Li, Yan
Wang, Juan
Qian, Dejian
Chen, Liang
Mo, Xiumei
Wang, Lei
Wang, Yan
Cui, Wenguo
Electrospun fibrous sponge via short fiber for mimicking 3D ECM
title Electrospun fibrous sponge via short fiber for mimicking 3D ECM
title_full Electrospun fibrous sponge via short fiber for mimicking 3D ECM
title_fullStr Electrospun fibrous sponge via short fiber for mimicking 3D ECM
title_full_unstemmed Electrospun fibrous sponge via short fiber for mimicking 3D ECM
title_short Electrospun fibrous sponge via short fiber for mimicking 3D ECM
title_sort electrospun fibrous sponge via short fiber for mimicking 3d ecm
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106196/
https://www.ncbi.nlm.nih.gov/pubmed/33964948
http://dx.doi.org/10.1186/s12951-021-00878-5
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