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Repatterning of the inflorescence meristem in Gerbera hybrida after wounding

The Asteraceae plant family is characterized by inflorescences, called flower heads or capitula that may combine hundreds of individual florets into a single flower-like structure. The florets are arranged in a regular phyllotactic pattern with Fibonacci numbers of left- and right-winding spirals. S...

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Autores principales: Zhang, Teng, Wang, Feng, Elomaa, Paula
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Singapore 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106577/
https://www.ncbi.nlm.nih.gov/pubmed/33543368
http://dx.doi.org/10.1007/s10265-021-01253-z
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author Zhang, Teng
Wang, Feng
Elomaa, Paula
author_facet Zhang, Teng
Wang, Feng
Elomaa, Paula
author_sort Zhang, Teng
collection PubMed
description The Asteraceae plant family is characterized by inflorescences, called flower heads or capitula that may combine hundreds of individual florets into a single flower-like structure. The florets are arranged in a regular phyllotactic pattern with Fibonacci numbers of left- and right-winding spirals. Such a pattern may be disrupted due to physical constraints or by wounding occurring during the early meristem development. Recovery from wounding re-establishes patterning although the mechanisms have remained elusive. In this study, we applied Gerbera hybrida as a model system and established methods to conduct wounding experiments either with syringe needles or using laser ablation combined with live imaging of head meristems. By revisiting the historical experiments in sunflower, we conducted wounding to transgenic auxin reporter lines of gerbera and followed the recovery of cellular growth and meristem patterning. We show that wounding disrupted the expression of the gerbera CLAVATA3 (GhCLV3) gene that marks the undifferentiated meristematic region and led to de novo re-initiation of patterning at the wound margin. During the recovery growth, three to five layers of elongated cells showing periclinal cell division planes and lacking auxin signal were formed at the wound rim. DR5 auxin signal was shown to localize and form regularly spaced maxima in a distance from the wound rim. Consequently, spiral pattern of contact parastichies was re-established by stacking of new auxin maxima on top of the previous ones. The developed methods facilitate future studies on understanding the molecular mechanisms of de novo patterning of meristems. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10265-021-01253-z.
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spelling pubmed-81065772021-05-24 Repatterning of the inflorescence meristem in Gerbera hybrida after wounding Zhang, Teng Wang, Feng Elomaa, Paula J Plant Res JPR Symposium The Asteraceae plant family is characterized by inflorescences, called flower heads or capitula that may combine hundreds of individual florets into a single flower-like structure. The florets are arranged in a regular phyllotactic pattern with Fibonacci numbers of left- and right-winding spirals. Such a pattern may be disrupted due to physical constraints or by wounding occurring during the early meristem development. Recovery from wounding re-establishes patterning although the mechanisms have remained elusive. In this study, we applied Gerbera hybrida as a model system and established methods to conduct wounding experiments either with syringe needles or using laser ablation combined with live imaging of head meristems. By revisiting the historical experiments in sunflower, we conducted wounding to transgenic auxin reporter lines of gerbera and followed the recovery of cellular growth and meristem patterning. We show that wounding disrupted the expression of the gerbera CLAVATA3 (GhCLV3) gene that marks the undifferentiated meristematic region and led to de novo re-initiation of patterning at the wound margin. During the recovery growth, three to five layers of elongated cells showing periclinal cell division planes and lacking auxin signal were formed at the wound rim. DR5 auxin signal was shown to localize and form regularly spaced maxima in a distance from the wound rim. Consequently, spiral pattern of contact parastichies was re-established by stacking of new auxin maxima on top of the previous ones. The developed methods facilitate future studies on understanding the molecular mechanisms of de novo patterning of meristems. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10265-021-01253-z. Springer Singapore 2021-02-04 2021 /pmc/articles/PMC8106577/ /pubmed/33543368 http://dx.doi.org/10.1007/s10265-021-01253-z Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle JPR Symposium
Zhang, Teng
Wang, Feng
Elomaa, Paula
Repatterning of the inflorescence meristem in Gerbera hybrida after wounding
title Repatterning of the inflorescence meristem in Gerbera hybrida after wounding
title_full Repatterning of the inflorescence meristem in Gerbera hybrida after wounding
title_fullStr Repatterning of the inflorescence meristem in Gerbera hybrida after wounding
title_full_unstemmed Repatterning of the inflorescence meristem in Gerbera hybrida after wounding
title_short Repatterning of the inflorescence meristem in Gerbera hybrida after wounding
title_sort repatterning of the inflorescence meristem in gerbera hybrida after wounding
topic JPR Symposium
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106577/
https://www.ncbi.nlm.nih.gov/pubmed/33543368
http://dx.doi.org/10.1007/s10265-021-01253-z
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