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De novo formation of early endosomes during Rab5-to-Rab7a transition
Rab5 and Rab7a are the main determinants of early and late endosomes and are important regulators of endosomal progression. The transport from early endosomes to late endosome seems to be regulated through an endosomal maturation switch, where Rab5 is gradually exchanged by Rab7a on the same endosom...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106955/ https://www.ncbi.nlm.nih.gov/pubmed/33737317 http://dx.doi.org/10.1242/jcs.254185 |
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author | Skjeldal, Frode Miltzow Haugen, Linda Hofstad Mateus, Duarte Frei, Dominik M. Rødseth, Anna Vik Hu, Xian Bakke, Oddmund |
author_facet | Skjeldal, Frode Miltzow Haugen, Linda Hofstad Mateus, Duarte Frei, Dominik M. Rødseth, Anna Vik Hu, Xian Bakke, Oddmund |
author_sort | Skjeldal, Frode Miltzow |
collection | PubMed |
description | Rab5 and Rab7a are the main determinants of early and late endosomes and are important regulators of endosomal progression. The transport from early endosomes to late endosome seems to be regulated through an endosomal maturation switch, where Rab5 is gradually exchanged by Rab7a on the same endosome. Here, we provide new insight into the mechanism of endosomal maturation, for which we have discovered a stepwise Rab5 detachment, sequentially regulated by Rab7a. The initial detachment of Rab5 is Rab7a independent and demonstrates a diffusion-like first-phase exchange between the cytosol and the endosomal membrane, and a second phase, in which Rab5 converges into specific domains that detach as a Rab5 indigenous endosome. Consequently, we show that early endosomal maturation regulated through the Rab5-to-Rab7a switch induces the formation of new fully functional Rab5-positive early endosomes. Progression through stepwise early endosomal maturation regulates the direction of transport and, concomitantly, the homeostasis of early endosomes. |
format | Online Article Text |
id | pubmed-8106955 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-81069552021-05-11 De novo formation of early endosomes during Rab5-to-Rab7a transition Skjeldal, Frode Miltzow Haugen, Linda Hofstad Mateus, Duarte Frei, Dominik M. Rødseth, Anna Vik Hu, Xian Bakke, Oddmund J Cell Sci Research Article Rab5 and Rab7a are the main determinants of early and late endosomes and are important regulators of endosomal progression. The transport from early endosomes to late endosome seems to be regulated through an endosomal maturation switch, where Rab5 is gradually exchanged by Rab7a on the same endosome. Here, we provide new insight into the mechanism of endosomal maturation, for which we have discovered a stepwise Rab5 detachment, sequentially regulated by Rab7a. The initial detachment of Rab5 is Rab7a independent and demonstrates a diffusion-like first-phase exchange between the cytosol and the endosomal membrane, and a second phase, in which Rab5 converges into specific domains that detach as a Rab5 indigenous endosome. Consequently, we show that early endosomal maturation regulated through the Rab5-to-Rab7a switch induces the formation of new fully functional Rab5-positive early endosomes. Progression through stepwise early endosomal maturation regulates the direction of transport and, concomitantly, the homeostasis of early endosomes. The Company of Biologists Ltd 2021-04-27 /pmc/articles/PMC8106955/ /pubmed/33737317 http://dx.doi.org/10.1242/jcs.254185 Text en © 2021. Published by The Company of Biologists Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Skjeldal, Frode Miltzow Haugen, Linda Hofstad Mateus, Duarte Frei, Dominik M. Rødseth, Anna Vik Hu, Xian Bakke, Oddmund De novo formation of early endosomes during Rab5-to-Rab7a transition |
title | De novo formation of early endosomes during Rab5-to-Rab7a transition |
title_full | De novo formation of early endosomes during Rab5-to-Rab7a transition |
title_fullStr | De novo formation of early endosomes during Rab5-to-Rab7a transition |
title_full_unstemmed | De novo formation of early endosomes during Rab5-to-Rab7a transition |
title_short | De novo formation of early endosomes during Rab5-to-Rab7a transition |
title_sort | de novo formation of early endosomes during rab5-to-rab7a transition |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8106955/ https://www.ncbi.nlm.nih.gov/pubmed/33737317 http://dx.doi.org/10.1242/jcs.254185 |
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