Cargando…

Staphylococcus aureus Ventilator-Associated Pneumonia: A Study of Bacterio-Epidemiological Profile and Virulence Factors

Ventilator-associated pneumonia (VAP) represents a major cause of nosocomial infections in the intensive care units in which Staphylococcus aureus is frequently involved. Better knowledge of this pathogen is required in order to enhance the patient’s treatment and care. In this article, we studied t...

Descripción completa

Detalles Bibliográficos
Autores principales: Tilouche, Lamia, Ben Dhia, Refka, Boughattas, Sameh, Ketata, Soumaya, Bouallegue, Olfa, Chaouch, Cherifa, Boujaafar, Noureddine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107017/
https://www.ncbi.nlm.nih.gov/pubmed/33969430
http://dx.doi.org/10.1007/s00284-021-02512-x
_version_ 1783689876266287104
author Tilouche, Lamia
Ben Dhia, Refka
Boughattas, Sameh
Ketata, Soumaya
Bouallegue, Olfa
Chaouch, Cherifa
Boujaafar, Noureddine
author_facet Tilouche, Lamia
Ben Dhia, Refka
Boughattas, Sameh
Ketata, Soumaya
Bouallegue, Olfa
Chaouch, Cherifa
Boujaafar, Noureddine
author_sort Tilouche, Lamia
collection PubMed
description Ventilator-associated pneumonia (VAP) represents a major cause of nosocomial infections in the intensive care units in which Staphylococcus aureus is frequently involved. Better knowledge of this pathogen is required in order to enhance the patient’s treatment and care. In this article, we studied the bacteriological profile and virulence factors of S. aureus-related VAP on a 3-year period. We included a collection of S. aureus strains (n = 35) isolated from respiratory samples from patients diagnosed with VAP in the intensive care units. We studied the bacteriological aspects and we searched for the presence of virulence factors (SpA, FnbpA, Hla, and PVL genes) in the strains, and we also studied the clinical and biological aspects of the infections. The average age of our patients was of 36 years and they were predominantly males (sex ratio = 3.37). A severe head trauma or a history of coma was noted in 73.43% of the patients. The average duration of ventilation was 29 days. Among the studied strains, five were Methicillin-resistant S. aureus of which three expressed the mecA gene. Overall, the Hla gene was detected in 85.7% of the strains and it was more prevalent in Methicillin-susceptible than Methicillin-resistant strains (93.3% versus 40%; P = 0.014). FnbpA, Spa, and PVL genes were detected, respectively, in 80%, 45.7%, and 20% of the strains. Therefore, our studied strains were essentially associated with the production of Hla and FnbpA genes. It is, however, important to elucidate their expression in order to establish their role in the VAP pathogenesis.
format Online
Article
Text
id pubmed-8107017
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-81070172021-05-10 Staphylococcus aureus Ventilator-Associated Pneumonia: A Study of Bacterio-Epidemiological Profile and Virulence Factors Tilouche, Lamia Ben Dhia, Refka Boughattas, Sameh Ketata, Soumaya Bouallegue, Olfa Chaouch, Cherifa Boujaafar, Noureddine Curr Microbiol Article Ventilator-associated pneumonia (VAP) represents a major cause of nosocomial infections in the intensive care units in which Staphylococcus aureus is frequently involved. Better knowledge of this pathogen is required in order to enhance the patient’s treatment and care. In this article, we studied the bacteriological profile and virulence factors of S. aureus-related VAP on a 3-year period. We included a collection of S. aureus strains (n = 35) isolated from respiratory samples from patients diagnosed with VAP in the intensive care units. We studied the bacteriological aspects and we searched for the presence of virulence factors (SpA, FnbpA, Hla, and PVL genes) in the strains, and we also studied the clinical and biological aspects of the infections. The average age of our patients was of 36 years and they were predominantly males (sex ratio = 3.37). A severe head trauma or a history of coma was noted in 73.43% of the patients. The average duration of ventilation was 29 days. Among the studied strains, five were Methicillin-resistant S. aureus of which three expressed the mecA gene. Overall, the Hla gene was detected in 85.7% of the strains and it was more prevalent in Methicillin-susceptible than Methicillin-resistant strains (93.3% versus 40%; P = 0.014). FnbpA, Spa, and PVL genes were detected, respectively, in 80%, 45.7%, and 20% of the strains. Therefore, our studied strains were essentially associated with the production of Hla and FnbpA genes. It is, however, important to elucidate their expression in order to establish their role in the VAP pathogenesis. Springer US 2021-05-10 2021 /pmc/articles/PMC8107017/ /pubmed/33969430 http://dx.doi.org/10.1007/s00284-021-02512-x Text en © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2021 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
Tilouche, Lamia
Ben Dhia, Refka
Boughattas, Sameh
Ketata, Soumaya
Bouallegue, Olfa
Chaouch, Cherifa
Boujaafar, Noureddine
Staphylococcus aureus Ventilator-Associated Pneumonia: A Study of Bacterio-Epidemiological Profile and Virulence Factors
title Staphylococcus aureus Ventilator-Associated Pneumonia: A Study of Bacterio-Epidemiological Profile and Virulence Factors
title_full Staphylococcus aureus Ventilator-Associated Pneumonia: A Study of Bacterio-Epidemiological Profile and Virulence Factors
title_fullStr Staphylococcus aureus Ventilator-Associated Pneumonia: A Study of Bacterio-Epidemiological Profile and Virulence Factors
title_full_unstemmed Staphylococcus aureus Ventilator-Associated Pneumonia: A Study of Bacterio-Epidemiological Profile and Virulence Factors
title_short Staphylococcus aureus Ventilator-Associated Pneumonia: A Study of Bacterio-Epidemiological Profile and Virulence Factors
title_sort staphylococcus aureus ventilator-associated pneumonia: a study of bacterio-epidemiological profile and virulence factors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107017/
https://www.ncbi.nlm.nih.gov/pubmed/33969430
http://dx.doi.org/10.1007/s00284-021-02512-x
work_keys_str_mv AT tilouchelamia staphylococcusaureusventilatorassociatedpneumoniaastudyofbacterioepidemiologicalprofileandvirulencefactors
AT bendhiarefka staphylococcusaureusventilatorassociatedpneumoniaastudyofbacterioepidemiologicalprofileandvirulencefactors
AT boughattassameh staphylococcusaureusventilatorassociatedpneumoniaastudyofbacterioepidemiologicalprofileandvirulencefactors
AT ketatasoumaya staphylococcusaureusventilatorassociatedpneumoniaastudyofbacterioepidemiologicalprofileandvirulencefactors
AT bouallegueolfa staphylococcusaureusventilatorassociatedpneumoniaastudyofbacterioepidemiologicalprofileandvirulencefactors
AT chaouchcherifa staphylococcusaureusventilatorassociatedpneumoniaastudyofbacterioepidemiologicalprofileandvirulencefactors
AT boujaafarnoureddine staphylococcusaureusventilatorassociatedpneumoniaastudyofbacterioepidemiologicalprofileandvirulencefactors