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Dysregulation of lncRNA‐CCRR contributes to brain metastasis of breast cancer by intercellular coupling via regulating connexin 43 expression
Cardiac conduction regulatory RNA (CCRR) is down‐regulated in the pathogenesis of heart failure (HF), which accordingly suppresses cardiac conduction while promoting arrhythmogenicity. Meanwhile, CX43 was reported to play a role in the pathogenesis of metastatic breast cancer and melanoma brain colo...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107087/ https://www.ncbi.nlm.nih.gov/pubmed/33793070 http://dx.doi.org/10.1111/jcmm.16455 |
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author | Li, Deheng Li, Liangdong Chen, Xin Zhou, Changshuai Hao, Bin Cao, Yiqun |
author_facet | Li, Deheng Li, Liangdong Chen, Xin Zhou, Changshuai Hao, Bin Cao, Yiqun |
author_sort | Li, Deheng |
collection | PubMed |
description | Cardiac conduction regulatory RNA (CCRR) is down‐regulated in the pathogenesis of heart failure (HF), which accordingly suppresses cardiac conduction while promoting arrhythmogenicity. Meanwhile, CX43 was reported to play a role in the pathogenesis of metastatic breast cancer and melanoma brain colonization. In this study, we studied the role of long non‐coding RNA CCRR and its interaction with CX43 in brain metastasis of breast cancer. Breast cancer patients were grouped according to the metastasis status. Real‐time PCR and IHC assay were used to measure the expression of lncRNA‐CCRR and CX43 in patients. Western blot was conducted to observe the effect of lncRNA‐CCRR on the expression of CX43 in MDA‐MB‐231BR and BT‐474BR cells. Compared with the non‐metastasis group, the mRNA expression of tissue lncRNA‐CCRR, cerebrospinal fluid (CSF) lncRNA‐CCRR, tissue CX43 and tissue protein expression of CX43 were both evidently up‐regulated in metastasis patients, especially in patients with brain metastasis. The expression of lncRNA‐CCRR was positively correlated with the up‐regulated expression of CX43. Moreover, CX43 expression was significantly lower in MDA‐MB‐231WT cells compared with that in MDA‐MB‐231BR cells. Also, the overexpression of lncRNA‐CCRR evidently increased dye transfer rate from astrocytes to MDA‐MB‐231BR/BT‐474BR cells but reduced lncRNA‐CCRR expression and suppressed the transmigration of MDA‐MB‐231BR/BT‐474BR cells in a blood‐brain barrier (BBB) model. In this study, we demonstrated that the presence of lncRNA‐CCRR could up‐regulate the expression of CX43, which promoted gap junction formation in brain metastasis of breast cancer. Accordingly, the communication between breast cancer cells and astrocytes was also promoted. |
format | Online Article Text |
id | pubmed-8107087 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81070872021-05-10 Dysregulation of lncRNA‐CCRR contributes to brain metastasis of breast cancer by intercellular coupling via regulating connexin 43 expression Li, Deheng Li, Liangdong Chen, Xin Zhou, Changshuai Hao, Bin Cao, Yiqun J Cell Mol Med Original Articles Cardiac conduction regulatory RNA (CCRR) is down‐regulated in the pathogenesis of heart failure (HF), which accordingly suppresses cardiac conduction while promoting arrhythmogenicity. Meanwhile, CX43 was reported to play a role in the pathogenesis of metastatic breast cancer and melanoma brain colonization. In this study, we studied the role of long non‐coding RNA CCRR and its interaction with CX43 in brain metastasis of breast cancer. Breast cancer patients were grouped according to the metastasis status. Real‐time PCR and IHC assay were used to measure the expression of lncRNA‐CCRR and CX43 in patients. Western blot was conducted to observe the effect of lncRNA‐CCRR on the expression of CX43 in MDA‐MB‐231BR and BT‐474BR cells. Compared with the non‐metastasis group, the mRNA expression of tissue lncRNA‐CCRR, cerebrospinal fluid (CSF) lncRNA‐CCRR, tissue CX43 and tissue protein expression of CX43 were both evidently up‐regulated in metastasis patients, especially in patients with brain metastasis. The expression of lncRNA‐CCRR was positively correlated with the up‐regulated expression of CX43. Moreover, CX43 expression was significantly lower in MDA‐MB‐231WT cells compared with that in MDA‐MB‐231BR cells. Also, the overexpression of lncRNA‐CCRR evidently increased dye transfer rate from astrocytes to MDA‐MB‐231BR/BT‐474BR cells but reduced lncRNA‐CCRR expression and suppressed the transmigration of MDA‐MB‐231BR/BT‐474BR cells in a blood‐brain barrier (BBB) model. In this study, we demonstrated that the presence of lncRNA‐CCRR could up‐regulate the expression of CX43, which promoted gap junction formation in brain metastasis of breast cancer. Accordingly, the communication between breast cancer cells and astrocytes was also promoted. John Wiley and Sons Inc. 2021-04-01 2021-05 /pmc/articles/PMC8107087/ /pubmed/33793070 http://dx.doi.org/10.1111/jcmm.16455 Text en © 2021 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Li, Deheng Li, Liangdong Chen, Xin Zhou, Changshuai Hao, Bin Cao, Yiqun Dysregulation of lncRNA‐CCRR contributes to brain metastasis of breast cancer by intercellular coupling via regulating connexin 43 expression |
title | Dysregulation of lncRNA‐CCRR contributes to brain metastasis of breast cancer by intercellular coupling via regulating connexin 43 expression |
title_full | Dysregulation of lncRNA‐CCRR contributes to brain metastasis of breast cancer by intercellular coupling via regulating connexin 43 expression |
title_fullStr | Dysregulation of lncRNA‐CCRR contributes to brain metastasis of breast cancer by intercellular coupling via regulating connexin 43 expression |
title_full_unstemmed | Dysregulation of lncRNA‐CCRR contributes to brain metastasis of breast cancer by intercellular coupling via regulating connexin 43 expression |
title_short | Dysregulation of lncRNA‐CCRR contributes to brain metastasis of breast cancer by intercellular coupling via regulating connexin 43 expression |
title_sort | dysregulation of lncrna‐ccrr contributes to brain metastasis of breast cancer by intercellular coupling via regulating connexin 43 expression |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107087/ https://www.ncbi.nlm.nih.gov/pubmed/33793070 http://dx.doi.org/10.1111/jcmm.16455 |
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