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Real-time volumetric reconstruction of biological dynamics with light-field microscopy and deep learning
Light-field microscopy has emerged as a technique of choice for high-speed volumetric imaging of fast biological processes. However, artefacts, non-uniform resolution, and a slow reconstruction speed have limited its full capabilities for in toto extraction of the dynamic spatiotemporal patterns in...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107123/ https://www.ncbi.nlm.nih.gov/pubmed/33574612 http://dx.doi.org/10.1038/s41592-021-01058-x |
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author | Wang, Zhaoqiang Zhu, Lanxin Zhang, Hao Li, Guo Yi, Chengqiang Li, Yi Yang, Yicong Ding, Yichen Zhen, Mei Gao, Shangbang Hsiai, Tzung K. Fei, Peng |
author_facet | Wang, Zhaoqiang Zhu, Lanxin Zhang, Hao Li, Guo Yi, Chengqiang Li, Yi Yang, Yicong Ding, Yichen Zhen, Mei Gao, Shangbang Hsiai, Tzung K. Fei, Peng |
author_sort | Wang, Zhaoqiang |
collection | PubMed |
description | Light-field microscopy has emerged as a technique of choice for high-speed volumetric imaging of fast biological processes. However, artefacts, non-uniform resolution, and a slow reconstruction speed have limited its full capabilities for in toto extraction of the dynamic spatiotemporal patterns in samples. Here, we combined a view-channel-depth (VCD) neural network with light-field microscopy to mitigate these limitations, yielding artefact-free three-dimensional image sequences with uniform spatial resolution and high video-rate reconstruction throughput. We imaged neuronal activities across moving C. elegans and blood flow in a beating zebrafish heart at single-cell resolution with volumetric imaging rates up to 200 Hz. |
format | Online Article Text |
id | pubmed-8107123 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
record_format | MEDLINE/PubMed |
spelling | pubmed-81071232021-08-11 Real-time volumetric reconstruction of biological dynamics with light-field microscopy and deep learning Wang, Zhaoqiang Zhu, Lanxin Zhang, Hao Li, Guo Yi, Chengqiang Li, Yi Yang, Yicong Ding, Yichen Zhen, Mei Gao, Shangbang Hsiai, Tzung K. Fei, Peng Nat Methods Article Light-field microscopy has emerged as a technique of choice for high-speed volumetric imaging of fast biological processes. However, artefacts, non-uniform resolution, and a slow reconstruction speed have limited its full capabilities for in toto extraction of the dynamic spatiotemporal patterns in samples. Here, we combined a view-channel-depth (VCD) neural network with light-field microscopy to mitigate these limitations, yielding artefact-free three-dimensional image sequences with uniform spatial resolution and high video-rate reconstruction throughput. We imaged neuronal activities across moving C. elegans and blood flow in a beating zebrafish heart at single-cell resolution with volumetric imaging rates up to 200 Hz. 2021-02-11 2021-05 /pmc/articles/PMC8107123/ /pubmed/33574612 http://dx.doi.org/10.1038/s41592-021-01058-x Text en http://www.nature.com/authors/editorial_policies/license.html#termsUsers may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Wang, Zhaoqiang Zhu, Lanxin Zhang, Hao Li, Guo Yi, Chengqiang Li, Yi Yang, Yicong Ding, Yichen Zhen, Mei Gao, Shangbang Hsiai, Tzung K. Fei, Peng Real-time volumetric reconstruction of biological dynamics with light-field microscopy and deep learning |
title | Real-time volumetric reconstruction of biological dynamics with light-field microscopy and deep learning |
title_full | Real-time volumetric reconstruction of biological dynamics with light-field microscopy and deep learning |
title_fullStr | Real-time volumetric reconstruction of biological dynamics with light-field microscopy and deep learning |
title_full_unstemmed | Real-time volumetric reconstruction of biological dynamics with light-field microscopy and deep learning |
title_short | Real-time volumetric reconstruction of biological dynamics with light-field microscopy and deep learning |
title_sort | real-time volumetric reconstruction of biological dynamics with light-field microscopy and deep learning |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107123/ https://www.ncbi.nlm.nih.gov/pubmed/33574612 http://dx.doi.org/10.1038/s41592-021-01058-x |
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