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Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein

Surface protein gp-120 of HIV-1 virus plays an important role in the infection of HIV-1, but detection of gp-120 during the early stage of infection is very difficult. Herein, we report a binding bioassay based on an RNA aptamer B40t77, which binds specifically to gp-120. The bioassay is built upon...

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Autores principales: Abbasi, Amna Didar, Hussain, Zakir, Liaqat, Usman, Arif, Dooa, Yang, Kun-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107394/
https://www.ncbi.nlm.nih.gov/pubmed/33981675
http://dx.doi.org/10.3389/fchem.2021.668870
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author Abbasi, Amna Didar
Hussain, Zakir
Liaqat, Usman
Arif, Dooa
Yang, Kun-Lin
author_facet Abbasi, Amna Didar
Hussain, Zakir
Liaqat, Usman
Arif, Dooa
Yang, Kun-Lin
author_sort Abbasi, Amna Didar
collection PubMed
description Surface protein gp-120 of HIV-1 virus plays an important role in the infection of HIV-1, but detection of gp-120 during the early stage of infection is very difficult. Herein, we report a binding bioassay based on an RNA aptamer B40t77, which binds specifically to gp-120. The bioassay is built upon a hydrophobic glass slide with surface immobilized gp-120. When the glass surface is incubated in a solution containing B40t77, the aptamer is able to bind to gp-120 specifically and remove it from the surface after a short incubation time of 30 min. The result of the binding event can be amplified by using liquid crystal (LC) into optical signals in the final step. By using this bioassay, we are able to detect as low as 1 μg/ml of gp-120 with high specificity within 30 min. No response is obtained when gp-120 is replaced by other protein such as bovine serum albumin (BSA). This is the first qualitative bioassay which provides a simple way for the detection of gp-120 with the naked eye. The assay is robust, low-cost and does not require additional labeling. Thus, the bioassay is potentially useful for the early detection of HIV-1 in resources-limited regions.
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spelling pubmed-81073942021-05-11 Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein Abbasi, Amna Didar Hussain, Zakir Liaqat, Usman Arif, Dooa Yang, Kun-Lin Front Chem Chemistry Surface protein gp-120 of HIV-1 virus plays an important role in the infection of HIV-1, but detection of gp-120 during the early stage of infection is very difficult. Herein, we report a binding bioassay based on an RNA aptamer B40t77, which binds specifically to gp-120. The bioassay is built upon a hydrophobic glass slide with surface immobilized gp-120. When the glass surface is incubated in a solution containing B40t77, the aptamer is able to bind to gp-120 specifically and remove it from the surface after a short incubation time of 30 min. The result of the binding event can be amplified by using liquid crystal (LC) into optical signals in the final step. By using this bioassay, we are able to detect as low as 1 μg/ml of gp-120 with high specificity within 30 min. No response is obtained when gp-120 is replaced by other protein such as bovine serum albumin (BSA). This is the first qualitative bioassay which provides a simple way for the detection of gp-120 with the naked eye. The assay is robust, low-cost and does not require additional labeling. Thus, the bioassay is potentially useful for the early detection of HIV-1 in resources-limited regions. Frontiers Media S.A. 2021-04-26 /pmc/articles/PMC8107394/ /pubmed/33981675 http://dx.doi.org/10.3389/fchem.2021.668870 Text en Copyright © 2021 Abbasi, Hussain, Liaqat, Arif and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Abbasi, Amna Didar
Hussain, Zakir
Liaqat, Usman
Arif, Dooa
Yang, Kun-Lin
Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein
title Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein
title_full Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein
title_fullStr Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein
title_full_unstemmed Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein
title_short Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein
title_sort liquid crystal based binding assay for detecting hiv-1 surface glycoprotein
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107394/
https://www.ncbi.nlm.nih.gov/pubmed/33981675
http://dx.doi.org/10.3389/fchem.2021.668870
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