Cargando…
Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein
Surface protein gp-120 of HIV-1 virus plays an important role in the infection of HIV-1, but detection of gp-120 during the early stage of infection is very difficult. Herein, we report a binding bioassay based on an RNA aptamer B40t77, which binds specifically to gp-120. The bioassay is built upon...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107394/ https://www.ncbi.nlm.nih.gov/pubmed/33981675 http://dx.doi.org/10.3389/fchem.2021.668870 |
_version_ | 1783689939958890496 |
---|---|
author | Abbasi, Amna Didar Hussain, Zakir Liaqat, Usman Arif, Dooa Yang, Kun-Lin |
author_facet | Abbasi, Amna Didar Hussain, Zakir Liaqat, Usman Arif, Dooa Yang, Kun-Lin |
author_sort | Abbasi, Amna Didar |
collection | PubMed |
description | Surface protein gp-120 of HIV-1 virus plays an important role in the infection of HIV-1, but detection of gp-120 during the early stage of infection is very difficult. Herein, we report a binding bioassay based on an RNA aptamer B40t77, which binds specifically to gp-120. The bioassay is built upon a hydrophobic glass slide with surface immobilized gp-120. When the glass surface is incubated in a solution containing B40t77, the aptamer is able to bind to gp-120 specifically and remove it from the surface after a short incubation time of 30 min. The result of the binding event can be amplified by using liquid crystal (LC) into optical signals in the final step. By using this bioassay, we are able to detect as low as 1 μg/ml of gp-120 with high specificity within 30 min. No response is obtained when gp-120 is replaced by other protein such as bovine serum albumin (BSA). This is the first qualitative bioassay which provides a simple way for the detection of gp-120 with the naked eye. The assay is robust, low-cost and does not require additional labeling. Thus, the bioassay is potentially useful for the early detection of HIV-1 in resources-limited regions. |
format | Online Article Text |
id | pubmed-8107394 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81073942021-05-11 Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein Abbasi, Amna Didar Hussain, Zakir Liaqat, Usman Arif, Dooa Yang, Kun-Lin Front Chem Chemistry Surface protein gp-120 of HIV-1 virus plays an important role in the infection of HIV-1, but detection of gp-120 during the early stage of infection is very difficult. Herein, we report a binding bioassay based on an RNA aptamer B40t77, which binds specifically to gp-120. The bioassay is built upon a hydrophobic glass slide with surface immobilized gp-120. When the glass surface is incubated in a solution containing B40t77, the aptamer is able to bind to gp-120 specifically and remove it from the surface after a short incubation time of 30 min. The result of the binding event can be amplified by using liquid crystal (LC) into optical signals in the final step. By using this bioassay, we are able to detect as low as 1 μg/ml of gp-120 with high specificity within 30 min. No response is obtained when gp-120 is replaced by other protein such as bovine serum albumin (BSA). This is the first qualitative bioassay which provides a simple way for the detection of gp-120 with the naked eye. The assay is robust, low-cost and does not require additional labeling. Thus, the bioassay is potentially useful for the early detection of HIV-1 in resources-limited regions. Frontiers Media S.A. 2021-04-26 /pmc/articles/PMC8107394/ /pubmed/33981675 http://dx.doi.org/10.3389/fchem.2021.668870 Text en Copyright © 2021 Abbasi, Hussain, Liaqat, Arif and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Abbasi, Amna Didar Hussain, Zakir Liaqat, Usman Arif, Dooa Yang, Kun-Lin Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein |
title | Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein |
title_full | Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein |
title_fullStr | Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein |
title_full_unstemmed | Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein |
title_short | Liquid Crystal Based Binding Assay for Detecting HIV-1 Surface Glycoprotein |
title_sort | liquid crystal based binding assay for detecting hiv-1 surface glycoprotein |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107394/ https://www.ncbi.nlm.nih.gov/pubmed/33981675 http://dx.doi.org/10.3389/fchem.2021.668870 |
work_keys_str_mv | AT abbasiamnadidar liquidcrystalbasedbindingassayfordetectinghiv1surfaceglycoprotein AT hussainzakir liquidcrystalbasedbindingassayfordetectinghiv1surfaceglycoprotein AT liaqatusman liquidcrystalbasedbindingassayfordetectinghiv1surfaceglycoprotein AT arifdooa liquidcrystalbasedbindingassayfordetectinghiv1surfaceglycoprotein AT yangkunlin liquidcrystalbasedbindingassayfordetectinghiv1surfaceglycoprotein |