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Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice

The mdx mouse phenotype aggravated by chronic exercise on a treadmill makes this murine model more reliable for the study of muscular dystrophy. Thus, to better assess the Tempol effect on dystrophic pathways, the analyses in this study were performed in the blood samples and diaphragm muscle from t...

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Autores principales: da Silva, Heloina Nathalliê Mariano, Covatti, Caroline, da Rocha, Guilherme Luiz, Mizobuti, Daniela Sayuri, Mâncio, Rafael Dias, Hermes, Túlio de Almeida, Kido, Larissa Akemi, Cagnon, Valéria Helena Alves, Pereira, Elaine Cristina Leite, Minatel, Elaine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107395/
https://www.ncbi.nlm.nih.gov/pubmed/33981250
http://dx.doi.org/10.3389/fphys.2021.649793
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author da Silva, Heloina Nathalliê Mariano
Covatti, Caroline
da Rocha, Guilherme Luiz
Mizobuti, Daniela Sayuri
Mâncio, Rafael Dias
Hermes, Túlio de Almeida
Kido, Larissa Akemi
Cagnon, Valéria Helena Alves
Pereira, Elaine Cristina Leite
Minatel, Elaine
author_facet da Silva, Heloina Nathalliê Mariano
Covatti, Caroline
da Rocha, Guilherme Luiz
Mizobuti, Daniela Sayuri
Mâncio, Rafael Dias
Hermes, Túlio de Almeida
Kido, Larissa Akemi
Cagnon, Valéria Helena Alves
Pereira, Elaine Cristina Leite
Minatel, Elaine
author_sort da Silva, Heloina Nathalliê Mariano
collection PubMed
description The mdx mouse phenotype aggravated by chronic exercise on a treadmill makes this murine model more reliable for the study of muscular dystrophy. Thus, to better assess the Tempol effect on dystrophic pathways, the analyses in this study were performed in the blood samples and diaphragm muscle from treadmill trained adult (7–11-weeks old) mdx animals. The mdx mice were divided into three groups: mdxSed, sedentary controls (n = 28); mdxEx, exercise-trained animals (n = 28); and mdxEx+T, exercise-trained animals with the Tempol treatment (n = 28). The results demonstrated that the Tempol treatment promoted muscle strength gain, prevented muscle damage, reduced the inflammatory process, oxidative stress, and angiogenesis regulator, and up regulated the activators of mitochondrial biogenesis. The main new findings of this study are that Tempol reduced the NF-κB and increased the PGC1-α and PPARδ levels in the exercise-trained-mdx mice, which are probably related to the ability of this antioxidant to scavenge excessive ROS. These results reinforce the use of Tempol as a potential therapeutic strategy in DMD.
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spelling pubmed-81073952021-05-11 Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice da Silva, Heloina Nathalliê Mariano Covatti, Caroline da Rocha, Guilherme Luiz Mizobuti, Daniela Sayuri Mâncio, Rafael Dias Hermes, Túlio de Almeida Kido, Larissa Akemi Cagnon, Valéria Helena Alves Pereira, Elaine Cristina Leite Minatel, Elaine Front Physiol Physiology The mdx mouse phenotype aggravated by chronic exercise on a treadmill makes this murine model more reliable for the study of muscular dystrophy. Thus, to better assess the Tempol effect on dystrophic pathways, the analyses in this study were performed in the blood samples and diaphragm muscle from treadmill trained adult (7–11-weeks old) mdx animals. The mdx mice were divided into three groups: mdxSed, sedentary controls (n = 28); mdxEx, exercise-trained animals (n = 28); and mdxEx+T, exercise-trained animals with the Tempol treatment (n = 28). The results demonstrated that the Tempol treatment promoted muscle strength gain, prevented muscle damage, reduced the inflammatory process, oxidative stress, and angiogenesis regulator, and up regulated the activators of mitochondrial biogenesis. The main new findings of this study are that Tempol reduced the NF-κB and increased the PGC1-α and PPARδ levels in the exercise-trained-mdx mice, which are probably related to the ability of this antioxidant to scavenge excessive ROS. These results reinforce the use of Tempol as a potential therapeutic strategy in DMD. Frontiers Media S.A. 2021-04-26 /pmc/articles/PMC8107395/ /pubmed/33981250 http://dx.doi.org/10.3389/fphys.2021.649793 Text en Copyright © 2021 Silva, Covatti, Rocha, Mizobuti, Mâncio, Hermes, Kido, Cagnon, Pereira and Minatel. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
da Silva, Heloina Nathalliê Mariano
Covatti, Caroline
da Rocha, Guilherme Luiz
Mizobuti, Daniela Sayuri
Mâncio, Rafael Dias
Hermes, Túlio de Almeida
Kido, Larissa Akemi
Cagnon, Valéria Helena Alves
Pereira, Elaine Cristina Leite
Minatel, Elaine
Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice
title Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice
title_full Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice
title_fullStr Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice
title_full_unstemmed Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice
title_short Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice
title_sort oxidative stress, inflammation, and activators of mitochondrial biogenesis: tempol targets in the diaphragm muscle of exercise trained-mdx mice
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107395/
https://www.ncbi.nlm.nih.gov/pubmed/33981250
http://dx.doi.org/10.3389/fphys.2021.649793
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