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Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice
The mdx mouse phenotype aggravated by chronic exercise on a treadmill makes this murine model more reliable for the study of muscular dystrophy. Thus, to better assess the Tempol effect on dystrophic pathways, the analyses in this study were performed in the blood samples and diaphragm muscle from t...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107395/ https://www.ncbi.nlm.nih.gov/pubmed/33981250 http://dx.doi.org/10.3389/fphys.2021.649793 |
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author | da Silva, Heloina Nathalliê Mariano Covatti, Caroline da Rocha, Guilherme Luiz Mizobuti, Daniela Sayuri Mâncio, Rafael Dias Hermes, Túlio de Almeida Kido, Larissa Akemi Cagnon, Valéria Helena Alves Pereira, Elaine Cristina Leite Minatel, Elaine |
author_facet | da Silva, Heloina Nathalliê Mariano Covatti, Caroline da Rocha, Guilherme Luiz Mizobuti, Daniela Sayuri Mâncio, Rafael Dias Hermes, Túlio de Almeida Kido, Larissa Akemi Cagnon, Valéria Helena Alves Pereira, Elaine Cristina Leite Minatel, Elaine |
author_sort | da Silva, Heloina Nathalliê Mariano |
collection | PubMed |
description | The mdx mouse phenotype aggravated by chronic exercise on a treadmill makes this murine model more reliable for the study of muscular dystrophy. Thus, to better assess the Tempol effect on dystrophic pathways, the analyses in this study were performed in the blood samples and diaphragm muscle from treadmill trained adult (7–11-weeks old) mdx animals. The mdx mice were divided into three groups: mdxSed, sedentary controls (n = 28); mdxEx, exercise-trained animals (n = 28); and mdxEx+T, exercise-trained animals with the Tempol treatment (n = 28). The results demonstrated that the Tempol treatment promoted muscle strength gain, prevented muscle damage, reduced the inflammatory process, oxidative stress, and angiogenesis regulator, and up regulated the activators of mitochondrial biogenesis. The main new findings of this study are that Tempol reduced the NF-κB and increased the PGC1-α and PPARδ levels in the exercise-trained-mdx mice, which are probably related to the ability of this antioxidant to scavenge excessive ROS. These results reinforce the use of Tempol as a potential therapeutic strategy in DMD. |
format | Online Article Text |
id | pubmed-8107395 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81073952021-05-11 Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice da Silva, Heloina Nathalliê Mariano Covatti, Caroline da Rocha, Guilherme Luiz Mizobuti, Daniela Sayuri Mâncio, Rafael Dias Hermes, Túlio de Almeida Kido, Larissa Akemi Cagnon, Valéria Helena Alves Pereira, Elaine Cristina Leite Minatel, Elaine Front Physiol Physiology The mdx mouse phenotype aggravated by chronic exercise on a treadmill makes this murine model more reliable for the study of muscular dystrophy. Thus, to better assess the Tempol effect on dystrophic pathways, the analyses in this study were performed in the blood samples and diaphragm muscle from treadmill trained adult (7–11-weeks old) mdx animals. The mdx mice were divided into three groups: mdxSed, sedentary controls (n = 28); mdxEx, exercise-trained animals (n = 28); and mdxEx+T, exercise-trained animals with the Tempol treatment (n = 28). The results demonstrated that the Tempol treatment promoted muscle strength gain, prevented muscle damage, reduced the inflammatory process, oxidative stress, and angiogenesis regulator, and up regulated the activators of mitochondrial biogenesis. The main new findings of this study are that Tempol reduced the NF-κB and increased the PGC1-α and PPARδ levels in the exercise-trained-mdx mice, which are probably related to the ability of this antioxidant to scavenge excessive ROS. These results reinforce the use of Tempol as a potential therapeutic strategy in DMD. Frontiers Media S.A. 2021-04-26 /pmc/articles/PMC8107395/ /pubmed/33981250 http://dx.doi.org/10.3389/fphys.2021.649793 Text en Copyright © 2021 Silva, Covatti, Rocha, Mizobuti, Mâncio, Hermes, Kido, Cagnon, Pereira and Minatel. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology da Silva, Heloina Nathalliê Mariano Covatti, Caroline da Rocha, Guilherme Luiz Mizobuti, Daniela Sayuri Mâncio, Rafael Dias Hermes, Túlio de Almeida Kido, Larissa Akemi Cagnon, Valéria Helena Alves Pereira, Elaine Cristina Leite Minatel, Elaine Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice |
title | Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice |
title_full | Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice |
title_fullStr | Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice |
title_full_unstemmed | Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice |
title_short | Oxidative Stress, Inflammation, and Activators of Mitochondrial Biogenesis: Tempol Targets in the Diaphragm Muscle of Exercise Trained-mdx Mice |
title_sort | oxidative stress, inflammation, and activators of mitochondrial biogenesis: tempol targets in the diaphragm muscle of exercise trained-mdx mice |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107395/ https://www.ncbi.nlm.nih.gov/pubmed/33981250 http://dx.doi.org/10.3389/fphys.2021.649793 |
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