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Breathing in vitro: Designs and applications of engineered lung models

The aim of this review is to provide a systematic design guideline to users, particularly engineers interested in developing and deploying lung models, and biologists seeking to identify a suitable platform for conducting in vitro experiments involving pulmonary cells or tissues. We first discuss th...

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Autores principales: Nossa, Roberta, Costa, Joana, Cacopardo, Ludovica, Ahluwalia, Arti
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107677/
https://www.ncbi.nlm.nih.gov/pubmed/33996022
http://dx.doi.org/10.1177/20417314211008696
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author Nossa, Roberta
Costa, Joana
Cacopardo, Ludovica
Ahluwalia, Arti
author_facet Nossa, Roberta
Costa, Joana
Cacopardo, Ludovica
Ahluwalia, Arti
author_sort Nossa, Roberta
collection PubMed
description The aim of this review is to provide a systematic design guideline to users, particularly engineers interested in developing and deploying lung models, and biologists seeking to identify a suitable platform for conducting in vitro experiments involving pulmonary cells or tissues. We first discuss the state of the art on lung in vitro models, describing the most simplistic and traditional ones. Then, we analyze in further detail the more complex dynamic engineered systems that either provide mechanical cues, or allow for more predictive exposure studies, or in some cases even both. This is followed by a dedicated section on microchips of the lung. Lastly, we present a critical discussion of the different characteristics of each type of system and the criteria which may help researchers select the most appropriate technology according to their specific requirements. Readers are encouraged to refer to the tables accompanying the different sections where comprehensive and quantitative information on the operating parameters and performance of the different systems reported in the literature is provided.
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spelling pubmed-81076772021-05-14 Breathing in vitro: Designs and applications of engineered lung models Nossa, Roberta Costa, Joana Cacopardo, Ludovica Ahluwalia, Arti J Tissue Eng Review The aim of this review is to provide a systematic design guideline to users, particularly engineers interested in developing and deploying lung models, and biologists seeking to identify a suitable platform for conducting in vitro experiments involving pulmonary cells or tissues. We first discuss the state of the art on lung in vitro models, describing the most simplistic and traditional ones. Then, we analyze in further detail the more complex dynamic engineered systems that either provide mechanical cues, or allow for more predictive exposure studies, or in some cases even both. This is followed by a dedicated section on microchips of the lung. Lastly, we present a critical discussion of the different characteristics of each type of system and the criteria which may help researchers select the most appropriate technology according to their specific requirements. Readers are encouraged to refer to the tables accompanying the different sections where comprehensive and quantitative information on the operating parameters and performance of the different systems reported in the literature is provided. SAGE Publications 2021-04-28 /pmc/articles/PMC8107677/ /pubmed/33996022 http://dx.doi.org/10.1177/20417314211008696 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution 4.0 License (https://creativecommons.org/licenses/by/4.0/) which permits any use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Review
Nossa, Roberta
Costa, Joana
Cacopardo, Ludovica
Ahluwalia, Arti
Breathing in vitro: Designs and applications of engineered lung models
title Breathing in vitro: Designs and applications of engineered lung models
title_full Breathing in vitro: Designs and applications of engineered lung models
title_fullStr Breathing in vitro: Designs and applications of engineered lung models
title_full_unstemmed Breathing in vitro: Designs and applications of engineered lung models
title_short Breathing in vitro: Designs and applications of engineered lung models
title_sort breathing in vitro: designs and applications of engineered lung models
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107677/
https://www.ncbi.nlm.nih.gov/pubmed/33996022
http://dx.doi.org/10.1177/20417314211008696
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