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Background mutation in strain RB2126 affects the locomotion behavior of flp-1 mutants
The FLP-1 neuropeptide is involved in locomotion, mechanosensation, and reproduction. Strain RB2126 is reported to contain the flp-1(ok2811) mutation, a prospective null allele. Here we report that strain RB2126, in addition to flp-1(ok2811), also contains two additional background mutations. One of...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Caltech Library
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107829/ https://www.ncbi.nlm.nih.gov/pubmed/33981966 http://dx.doi.org/10.17912/micropub.biology.000393 |
Sumario: | The FLP-1 neuropeptide is involved in locomotion, mechanosensation, and reproduction. Strain RB2126 is reported to contain the flp-1(ok2811) mutation, a prospective null allele. Here we report that strain RB2126, in addition to flp-1(ok2811), also contains two additional background mutations. One of the mutations gives the animals a constitutive dauer phenotype (Daf-c), and complementation testing confirms that it is in the Daf-c gene daf-11. The second mutation is isolated based on the fact that it partially suppresses the elevated body curvature of flp-1(ok2811) mutants, but it remains uncloned. The updated genotype of RB2126 strain is flp-1(ok2811); daf-11(yak155); yak156. |
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