Cargando…
B regulatory cells and monocyte subpopulations in patients with chronic graft-vs-host disease
AIM: To assess the correlations of B regulatory cells (Bregs) and monocyte subsets in peripheral blood with the National Institutes of Health (NIH)-consensus-defined clinical manifestations of chronic graft-vs-host disease (cGvHD), in an attempt to establish their role as cellular biomarkers. METHOD...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Croatian Medical Schools
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107991/ https://www.ncbi.nlm.nih.gov/pubmed/33938655 http://dx.doi.org/10.3325/cmj.2021.62.154 |
_version_ | 1783690051425665024 |
---|---|
author | Babić, Antonija Kurić, Lejla Zelić, Ana Kerep, Desnica, Lana Lelas, Antonela Milošević, Milan Serventi-Seiwerth, Ranka Duraković, Nadira Peric, Zinaida Mravak, Marinka Stipetić, Bilic, Ervina Čeović, Romana Barešić, Marko Vukić, Tamara Ljubas Kelečić, Dina Mazić, Sanja Bojanić, Ines Hećimović, Ana Bilic, Ernest Zadro, Renata Vrhovac, Radovan Pavletic, Steven Z. Batinić, Drago Pulanić, Dražen |
author_facet | Babić, Antonija Kurić, Lejla Zelić, Ana Kerep, Desnica, Lana Lelas, Antonela Milošević, Milan Serventi-Seiwerth, Ranka Duraković, Nadira Peric, Zinaida Mravak, Marinka Stipetić, Bilic, Ervina Čeović, Romana Barešić, Marko Vukić, Tamara Ljubas Kelečić, Dina Mazić, Sanja Bojanić, Ines Hećimović, Ana Bilic, Ernest Zadro, Renata Vrhovac, Radovan Pavletic, Steven Z. Batinić, Drago Pulanić, Dražen |
author_sort | Babić, Antonija |
collection | PubMed |
description | AIM: To assess the correlations of B regulatory cells (Bregs) and monocyte subsets in peripheral blood with the National Institutes of Health (NIH)-consensus-defined clinical manifestations of chronic graft-vs-host disease (cGvHD), in an attempt to establish their role as cellular biomarkers. METHODS: This multidisciplinary prospective study enrolled adult cGVHD patients treated in the University Hospital Center Zagreb and University of Zagreb School of Medicine. Immunophenotypic subpopulations of CD24(high)CD38(high) Bregs (CD27-, CD27+, and total) and monocyte (classical, intermediate, and non-classical) counts were correlated with demographic, transplant, and cGVHD-related data. Bivariate correlation analysis was performed to evaluate the correlations between Bregs and monocytes subsets and cGVHD organ involvement, as well as cGVHD severity and immunosuppression intensity. RESULTS: Twenty-two adult patients (54.5% female) with cGVHD were enrolled. The median (range) age was 44.5 years (24-65). All patients were transplanted for hematologic malignancies and 40.9% had severe NIH cGVHD global score. The median time from cGVHD diagnosis to the analysis was 16.6 months (0-176). The organ most frequently affected with cGVHD were the eyes (68.2%), skin (45.5%), lungs (45.5%), and liver (40.9%). Lower total and CD27-Bregs counts were correlated with worse cGVHD severity, higher immunosuppression intensity, and lung cGVHD, in terms of cell count, but also with skin cGVHD, in terms of percentages. Patients with liver and joint/fascia cGVHD had a lower percentage of non-classical monocytes and patients with more severe global NIH score had a higher classical monocytes count. CONCLUSION: Different organs affected by cGVHD are differently associated with different subpopulations of Bregs and monocytes. |
format | Online Article Text |
id | pubmed-8107991 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Croatian Medical Schools |
record_format | MEDLINE/PubMed |
spelling | pubmed-81079912021-05-17 B regulatory cells and monocyte subpopulations in patients with chronic graft-vs-host disease Babić, Antonija Kurić, Lejla Zelić, Ana Kerep, Desnica, Lana Lelas, Antonela Milošević, Milan Serventi-Seiwerth, Ranka Duraković, Nadira Peric, Zinaida Mravak, Marinka Stipetić, Bilic, Ervina Čeović, Romana Barešić, Marko Vukić, Tamara Ljubas Kelečić, Dina Mazić, Sanja Bojanić, Ines Hećimović, Ana Bilic, Ernest Zadro, Renata Vrhovac, Radovan Pavletic, Steven Z. Batinić, Drago Pulanić, Dražen Croat Med J Research Article AIM: To assess the correlations of B regulatory cells (Bregs) and monocyte subsets in peripheral blood with the National Institutes of Health (NIH)-consensus-defined clinical manifestations of chronic graft-vs-host disease (cGvHD), in an attempt to establish their role as cellular biomarkers. METHODS: This multidisciplinary prospective study enrolled adult cGVHD patients treated in the University Hospital Center Zagreb and University of Zagreb School of Medicine. Immunophenotypic subpopulations of CD24(high)CD38(high) Bregs (CD27-, CD27+, and total) and monocyte (classical, intermediate, and non-classical) counts were correlated with demographic, transplant, and cGVHD-related data. Bivariate correlation analysis was performed to evaluate the correlations between Bregs and monocytes subsets and cGVHD organ involvement, as well as cGVHD severity and immunosuppression intensity. RESULTS: Twenty-two adult patients (54.5% female) with cGVHD were enrolled. The median (range) age was 44.5 years (24-65). All patients were transplanted for hematologic malignancies and 40.9% had severe NIH cGVHD global score. The median time from cGVHD diagnosis to the analysis was 16.6 months (0-176). The organ most frequently affected with cGVHD were the eyes (68.2%), skin (45.5%), lungs (45.5%), and liver (40.9%). Lower total and CD27-Bregs counts were correlated with worse cGVHD severity, higher immunosuppression intensity, and lung cGVHD, in terms of cell count, but also with skin cGVHD, in terms of percentages. Patients with liver and joint/fascia cGVHD had a lower percentage of non-classical monocytes and patients with more severe global NIH score had a higher classical monocytes count. CONCLUSION: Different organs affected by cGVHD are differently associated with different subpopulations of Bregs and monocytes. Croatian Medical Schools 2021-04 /pmc/articles/PMC8107991/ /pubmed/33938655 http://dx.doi.org/10.3325/cmj.2021.62.154 Text en Copyright © 2021 by the Croatian Medical Journal. All rights reserved. https://creativecommons.org/licenses/by/2.5/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Babić, Antonija Kurić, Lejla Zelić, Ana Kerep, Desnica, Lana Lelas, Antonela Milošević, Milan Serventi-Seiwerth, Ranka Duraković, Nadira Peric, Zinaida Mravak, Marinka Stipetić, Bilic, Ervina Čeović, Romana Barešić, Marko Vukić, Tamara Ljubas Kelečić, Dina Mazić, Sanja Bojanić, Ines Hećimović, Ana Bilic, Ernest Zadro, Renata Vrhovac, Radovan Pavletic, Steven Z. Batinić, Drago Pulanić, Dražen B regulatory cells and monocyte subpopulations in patients with chronic graft-vs-host disease |
title | B regulatory cells and monocyte subpopulations in patients with chronic graft-vs-host disease |
title_full | B regulatory cells and monocyte subpopulations in patients with chronic graft-vs-host disease |
title_fullStr | B regulatory cells and monocyte subpopulations in patients with chronic graft-vs-host disease |
title_full_unstemmed | B regulatory cells and monocyte subpopulations in patients with chronic graft-vs-host disease |
title_short | B regulatory cells and monocyte subpopulations in patients with chronic graft-vs-host disease |
title_sort | b regulatory cells and monocyte subpopulations in patients with chronic graft-vs-host disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8107991/ https://www.ncbi.nlm.nih.gov/pubmed/33938655 http://dx.doi.org/10.3325/cmj.2021.62.154 |
work_keys_str_mv | AT babicantonija bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT kuriclejla bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT zelicana bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT kerep bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT desnicalana bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT lelasantonela bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT milosevicmilan bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT serventiseiwerthranka bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT durakovicnadira bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT periczinaida bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT mravakmarinka bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT stipetic bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT bilicervina bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT ceovicromana bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT baresicmarko bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT vukictamara bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT ljubaskelecicdina bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT mazicsanja bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT bojanicines bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT hecimovicana bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT bilicernest bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT zadrorenata bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT vrhovacradovan bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT pavleticstevenz bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT batinicdrago bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease AT pulanicdrazen bregulatorycellsandmonocytesubpopulationsinpatientswithchronicgraftvshostdisease |