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Sevoflurane represses the migration and invasion of gastric cancer cells by regulating forkhead box protein 3

OBJECTIVE: Previous studies suggested that sevoflurane exerts anti-proliferative, anti-migratory, and anti-invasive effects on cancer cells. To determine the role of sevoflurane on gastric cancer (GC) progression, we evaluated its effects on the proliferation, migration, and invasion of SGC7901, AGS...

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Autores principales: Yong, Fangfang, Wang, Hemei, Li, Chao, Jia, Huiqun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108091/
https://www.ncbi.nlm.nih.gov/pubmed/33906525
http://dx.doi.org/10.1177/03000605211005936
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author Yong, Fangfang
Wang, Hemei
Li, Chao
Jia, Huiqun
author_facet Yong, Fangfang
Wang, Hemei
Li, Chao
Jia, Huiqun
author_sort Yong, Fangfang
collection PubMed
description OBJECTIVE: Previous studies suggested that sevoflurane exerts anti-proliferative, anti-migratory, and anti-invasive effects on cancer cells. To determine the role of sevoflurane on gastric cancer (GC) progression, we evaluated its effects on the proliferation, migration, and invasion of SGC7901, AGS, and MGC803 GC cells. METHODS: GC cells were exposed to different concentrations of sevoflurane (1.7, 3.4, or 5.1% v/v). Cell viability, migration, and invasion were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Transwell assays. Immunohistochemical staining and immunoblotting were performed to analyze forkhead box protein 3 (FOXP3) protein expression in tissue specimens and cell lines, respectively. RESULTS: FOXP3 was downregulated in human GC specimens and cell lines. Functionally, FOXP3 overexpression significantly inhibited the proliferation, migration, and invasion of GC cells and accelerated their apoptosis. Moreover, sevoflurane significantly blocked GC cell migration and invasion compared with the findings in the control group. However, FOXP3 silencing neutralized sevoflurane-induced apoptosis and the inhibition of GC cell migration and invasion. Sevoflurane-induced apoptosis and the suppression of migration and invasion might be associated with FOXP3 overactivation in GC cells. CONCLUSIONS: Sevoflurane activated FOXP3 and prevented GC progression via inhibiting cell migration and invasion in vitro.
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spelling pubmed-81080912021-05-14 Sevoflurane represses the migration and invasion of gastric cancer cells by regulating forkhead box protein 3 Yong, Fangfang Wang, Hemei Li, Chao Jia, Huiqun J Int Med Res Pre-Clinical Research Report OBJECTIVE: Previous studies suggested that sevoflurane exerts anti-proliferative, anti-migratory, and anti-invasive effects on cancer cells. To determine the role of sevoflurane on gastric cancer (GC) progression, we evaluated its effects on the proliferation, migration, and invasion of SGC7901, AGS, and MGC803 GC cells. METHODS: GC cells were exposed to different concentrations of sevoflurane (1.7, 3.4, or 5.1% v/v). Cell viability, migration, and invasion were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Transwell assays. Immunohistochemical staining and immunoblotting were performed to analyze forkhead box protein 3 (FOXP3) protein expression in tissue specimens and cell lines, respectively. RESULTS: FOXP3 was downregulated in human GC specimens and cell lines. Functionally, FOXP3 overexpression significantly inhibited the proliferation, migration, and invasion of GC cells and accelerated their apoptosis. Moreover, sevoflurane significantly blocked GC cell migration and invasion compared with the findings in the control group. However, FOXP3 silencing neutralized sevoflurane-induced apoptosis and the inhibition of GC cell migration and invasion. Sevoflurane-induced apoptosis and the suppression of migration and invasion might be associated with FOXP3 overactivation in GC cells. CONCLUSIONS: Sevoflurane activated FOXP3 and prevented GC progression via inhibiting cell migration and invasion in vitro. SAGE Publications 2021-04-27 /pmc/articles/PMC8108091/ /pubmed/33906525 http://dx.doi.org/10.1177/03000605211005936 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Pre-Clinical Research Report
Yong, Fangfang
Wang, Hemei
Li, Chao
Jia, Huiqun
Sevoflurane represses the migration and invasion of gastric cancer cells by regulating forkhead box protein 3
title Sevoflurane represses the migration and invasion of gastric cancer cells by regulating forkhead box protein 3
title_full Sevoflurane represses the migration and invasion of gastric cancer cells by regulating forkhead box protein 3
title_fullStr Sevoflurane represses the migration and invasion of gastric cancer cells by regulating forkhead box protein 3
title_full_unstemmed Sevoflurane represses the migration and invasion of gastric cancer cells by regulating forkhead box protein 3
title_short Sevoflurane represses the migration and invasion of gastric cancer cells by regulating forkhead box protein 3
title_sort sevoflurane represses the migration and invasion of gastric cancer cells by regulating forkhead box protein 3
topic Pre-Clinical Research Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108091/
https://www.ncbi.nlm.nih.gov/pubmed/33906525
http://dx.doi.org/10.1177/03000605211005936
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