Cargando…

SOX9 is associated with advanced T-stages of clinical stage II colon cancer in young Mexican patients

Colorectal cancer (CRC) is one of the most common malignancies worldwide and includes colon cancer (CC) and rectal cancer (RC). Regarding CC, the development of novel molecular biomarkers for the accurate diagnosis and prognosis, as well as the identification of novel targets for therapeutic interve...

Descripción completa

Detalles Bibliográficos
Autores principales: Lizárraga-Verdugo, Erik, Carmona, Tatiana Galicia, Ramos-Payan, Rosalío, Avendaño-Félix, Mariana, Bermúdez, Mercedes, Parra-Niebla, Maryelv, López-Camarillo, César, Fernandez-Figueroa, Edith, Lino-Silva, Leonardo, Saavedra, Herminia Arvelo, Vela-Sarmiento, Itzel, Ovando, Roberto Cárdenas, Ruíz-García, Erika, Aguilar-Medina, Maribel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108287/
https://www.ncbi.nlm.nih.gov/pubmed/33981359
http://dx.doi.org/10.3892/ol.2021.12758
_version_ 1783690100434010112
author Lizárraga-Verdugo, Erik
Carmona, Tatiana Galicia
Ramos-Payan, Rosalío
Avendaño-Félix, Mariana
Bermúdez, Mercedes
Parra-Niebla, Maryelv
López-Camarillo, César
Fernandez-Figueroa, Edith
Lino-Silva, Leonardo
Saavedra, Herminia Arvelo
Vela-Sarmiento, Itzel
Ovando, Roberto Cárdenas
Ruíz-García, Erika
Aguilar-Medina, Maribel
author_facet Lizárraga-Verdugo, Erik
Carmona, Tatiana Galicia
Ramos-Payan, Rosalío
Avendaño-Félix, Mariana
Bermúdez, Mercedes
Parra-Niebla, Maryelv
López-Camarillo, César
Fernandez-Figueroa, Edith
Lino-Silva, Leonardo
Saavedra, Herminia Arvelo
Vela-Sarmiento, Itzel
Ovando, Roberto Cárdenas
Ruíz-García, Erika
Aguilar-Medina, Maribel
author_sort Lizárraga-Verdugo, Erik
collection PubMed
description Colorectal cancer (CRC) is one of the most common malignancies worldwide and includes colon cancer (CC) and rectal cancer (RC). Regarding CC, the development of novel molecular biomarkers for the accurate diagnosis and prognosis, as well as the identification of novel targets for therapeutic intervention, are urgently needed. SRY-related high-mobility group box 9 (SOX9), a transcription factor, is involved in development, and has been associated with the progression of human cancer. However, its underlying clinical and functional effects in CRC have not been fully understood. Therefore, the present study aimed to evaluate the clinical and functional relevance of SOX9 expression in CC. The expression of SOX9 in tumor tissues was evaluated in 97 biopsies from Mexican patients with CC with early-stage I and II disease by immunohistochemistry (IHC). In addition, SOX9 silencing in the HCT116 cell line was performed using specific small interfering RNAs, while downregulation efficiency was verified by reverse transcription-quantitative PCR and immunofluorescence. Spheroid-formation assay was carried out using ultra-low attachment plates. The IHC results showed that SOX9 was upregulated in patients with stage II (91%) and advanced T3 stage (67%) CC. Interestingly, higher SOX9 expression was associated with clinical stage, tumor size and tumor location. Furthermore, increased SOX9 expression was found in relapsed cases with local tumors; however, it was not associated with increased survival probability. Additionally, functional analysis indicated that SOX9 silencing significantly attenuated the sphere-formation capability of HCT116 cells. The present study was the first to evaluate the expression levels of SOX9 in Mexican patients diagnosed with early-stage CC. The aforementioned findings indicated that high SOX9 expression could play an important role in tumorigenesis and be associated with advanced T-stages of clinical-stage II patients, but not with relapse-free survival.
format Online
Article
Text
id pubmed-8108287
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-81082872021-05-11 SOX9 is associated with advanced T-stages of clinical stage II colon cancer in young Mexican patients Lizárraga-Verdugo, Erik Carmona, Tatiana Galicia Ramos-Payan, Rosalío Avendaño-Félix, Mariana Bermúdez, Mercedes Parra-Niebla, Maryelv López-Camarillo, César Fernandez-Figueroa, Edith Lino-Silva, Leonardo Saavedra, Herminia Arvelo Vela-Sarmiento, Itzel Ovando, Roberto Cárdenas Ruíz-García, Erika Aguilar-Medina, Maribel Oncol Lett Articles Colorectal cancer (CRC) is one of the most common malignancies worldwide and includes colon cancer (CC) and rectal cancer (RC). Regarding CC, the development of novel molecular biomarkers for the accurate diagnosis and prognosis, as well as the identification of novel targets for therapeutic intervention, are urgently needed. SRY-related high-mobility group box 9 (SOX9), a transcription factor, is involved in development, and has been associated with the progression of human cancer. However, its underlying clinical and functional effects in CRC have not been fully understood. Therefore, the present study aimed to evaluate the clinical and functional relevance of SOX9 expression in CC. The expression of SOX9 in tumor tissues was evaluated in 97 biopsies from Mexican patients with CC with early-stage I and II disease by immunohistochemistry (IHC). In addition, SOX9 silencing in the HCT116 cell line was performed using specific small interfering RNAs, while downregulation efficiency was verified by reverse transcription-quantitative PCR and immunofluorescence. Spheroid-formation assay was carried out using ultra-low attachment plates. The IHC results showed that SOX9 was upregulated in patients with stage II (91%) and advanced T3 stage (67%) CC. Interestingly, higher SOX9 expression was associated with clinical stage, tumor size and tumor location. Furthermore, increased SOX9 expression was found in relapsed cases with local tumors; however, it was not associated with increased survival probability. Additionally, functional analysis indicated that SOX9 silencing significantly attenuated the sphere-formation capability of HCT116 cells. The present study was the first to evaluate the expression levels of SOX9 in Mexican patients diagnosed with early-stage CC. The aforementioned findings indicated that high SOX9 expression could play an important role in tumorigenesis and be associated with advanced T-stages of clinical-stage II patients, but not with relapse-free survival. D.A. Spandidos 2021-07 2021-04-27 /pmc/articles/PMC8108287/ /pubmed/33981359 http://dx.doi.org/10.3892/ol.2021.12758 Text en Copyright: © Lizárraga-Verdugo et al. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Lizárraga-Verdugo, Erik
Carmona, Tatiana Galicia
Ramos-Payan, Rosalío
Avendaño-Félix, Mariana
Bermúdez, Mercedes
Parra-Niebla, Maryelv
López-Camarillo, César
Fernandez-Figueroa, Edith
Lino-Silva, Leonardo
Saavedra, Herminia Arvelo
Vela-Sarmiento, Itzel
Ovando, Roberto Cárdenas
Ruíz-García, Erika
Aguilar-Medina, Maribel
SOX9 is associated with advanced T-stages of clinical stage II colon cancer in young Mexican patients
title SOX9 is associated with advanced T-stages of clinical stage II colon cancer in young Mexican patients
title_full SOX9 is associated with advanced T-stages of clinical stage II colon cancer in young Mexican patients
title_fullStr SOX9 is associated with advanced T-stages of clinical stage II colon cancer in young Mexican patients
title_full_unstemmed SOX9 is associated with advanced T-stages of clinical stage II colon cancer in young Mexican patients
title_short SOX9 is associated with advanced T-stages of clinical stage II colon cancer in young Mexican patients
title_sort sox9 is associated with advanced t-stages of clinical stage ii colon cancer in young mexican patients
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108287/
https://www.ncbi.nlm.nih.gov/pubmed/33981359
http://dx.doi.org/10.3892/ol.2021.12758
work_keys_str_mv AT lizarragaverdugoerik sox9isassociatedwithadvancedtstagesofclinicalstageiicoloncancerinyoungmexicanpatients
AT carmonatatianagalicia sox9isassociatedwithadvancedtstagesofclinicalstageiicoloncancerinyoungmexicanpatients
AT ramospayanrosalio sox9isassociatedwithadvancedtstagesofclinicalstageiicoloncancerinyoungmexicanpatients
AT avendanofelixmariana sox9isassociatedwithadvancedtstagesofclinicalstageiicoloncancerinyoungmexicanpatients
AT bermudezmercedes sox9isassociatedwithadvancedtstagesofclinicalstageiicoloncancerinyoungmexicanpatients
AT parranieblamaryelv sox9isassociatedwithadvancedtstagesofclinicalstageiicoloncancerinyoungmexicanpatients
AT lopezcamarillocesar sox9isassociatedwithadvancedtstagesofclinicalstageiicoloncancerinyoungmexicanpatients
AT fernandezfigueroaedith sox9isassociatedwithadvancedtstagesofclinicalstageiicoloncancerinyoungmexicanpatients
AT linosilvaleonardo sox9isassociatedwithadvancedtstagesofclinicalstageiicoloncancerinyoungmexicanpatients
AT saavedraherminiaarvelo sox9isassociatedwithadvancedtstagesofclinicalstageiicoloncancerinyoungmexicanpatients
AT velasarmientoitzel sox9isassociatedwithadvancedtstagesofclinicalstageiicoloncancerinyoungmexicanpatients
AT ovandorobertocardenas sox9isassociatedwithadvancedtstagesofclinicalstageiicoloncancerinyoungmexicanpatients
AT ruizgarciaerika sox9isassociatedwithadvancedtstagesofclinicalstageiicoloncancerinyoungmexicanpatients
AT aguilarmedinamaribel sox9isassociatedwithadvancedtstagesofclinicalstageiicoloncancerinyoungmexicanpatients