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Direct and indirect regulation of Pom1 cell size pathway by the protein phosphatase 2C Ptc1
The fission yeast cells Schizosaccharomyces pombe divide at constant cell size regulated by environmental stimuli. An important pathway of cell size control involves the membrane-associated DYRK-family kinase Pom1, which forms decreasing concentration gradients from cell poles and inhibits mitotic i...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108516/ https://www.ncbi.nlm.nih.gov/pubmed/33625871 http://dx.doi.org/10.1091/mbc.E20-08-0508 |
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author | Gerganova, Veneta Bhatia, Payal Vincenzetti, Vincent Martin, Sophie G. |
author_facet | Gerganova, Veneta Bhatia, Payal Vincenzetti, Vincent Martin, Sophie G. |
author_sort | Gerganova, Veneta |
collection | PubMed |
description | The fission yeast cells Schizosaccharomyces pombe divide at constant cell size regulated by environmental stimuli. An important pathway of cell size control involves the membrane-associated DYRK-family kinase Pom1, which forms decreasing concentration gradients from cell poles and inhibits mitotic inducers at midcell. Here, we identify the phosphatase 2C Ptc1 as negative regulator of Pom1. Ptc1 localizes to cell poles in a manner dependent on polarity and cell-wall integrity factors. We show that Ptc1 directly binds Pom1 and can dephosphorylate it in vitro but modulates Pom1 localization indirectly upon growth in low-glucose conditions by influencing microtubule stability. Thus, Ptc1 phosphatase plays both direct and indirect roles in the Pom1 cell size control pathway. |
format | Online Article Text |
id | pubmed-8108516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-81085162021-06-30 Direct and indirect regulation of Pom1 cell size pathway by the protein phosphatase 2C Ptc1 Gerganova, Veneta Bhatia, Payal Vincenzetti, Vincent Martin, Sophie G. Mol Biol Cell Articles The fission yeast cells Schizosaccharomyces pombe divide at constant cell size regulated by environmental stimuli. An important pathway of cell size control involves the membrane-associated DYRK-family kinase Pom1, which forms decreasing concentration gradients from cell poles and inhibits mitotic inducers at midcell. Here, we identify the phosphatase 2C Ptc1 as negative regulator of Pom1. Ptc1 localizes to cell poles in a manner dependent on polarity and cell-wall integrity factors. We show that Ptc1 directly binds Pom1 and can dephosphorylate it in vitro but modulates Pom1 localization indirectly upon growth in low-glucose conditions by influencing microtubule stability. Thus, Ptc1 phosphatase plays both direct and indirect roles in the Pom1 cell size control pathway. The American Society for Cell Biology 2021-04-15 /pmc/articles/PMC8108516/ /pubmed/33625871 http://dx.doi.org/10.1091/mbc.E20-08-0508 Text en © 2021 Gerganova et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. https://creativecommons.org/licenses/by-nc-sa/3.0/This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License. |
spellingShingle | Articles Gerganova, Veneta Bhatia, Payal Vincenzetti, Vincent Martin, Sophie G. Direct and indirect regulation of Pom1 cell size pathway by the protein phosphatase 2C Ptc1 |
title | Direct and indirect regulation of Pom1 cell size pathway by the protein phosphatase 2C Ptc1 |
title_full | Direct and indirect regulation of Pom1 cell size pathway by the protein phosphatase 2C Ptc1 |
title_fullStr | Direct and indirect regulation of Pom1 cell size pathway by the protein phosphatase 2C Ptc1 |
title_full_unstemmed | Direct and indirect regulation of Pom1 cell size pathway by the protein phosphatase 2C Ptc1 |
title_short | Direct and indirect regulation of Pom1 cell size pathway by the protein phosphatase 2C Ptc1 |
title_sort | direct and indirect regulation of pom1 cell size pathway by the protein phosphatase 2c ptc1 |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108516/ https://www.ncbi.nlm.nih.gov/pubmed/33625871 http://dx.doi.org/10.1091/mbc.E20-08-0508 |
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