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Diffusion of kinesin motors on cargo can enhance binding and run lengths during intracellular transport

Cellular cargoes, including lipid droplets and mitochondria, are transported along microtubules using molecular motors such as kinesins. Many experimental and computational studies focused on cargoes with rigidly attached motors, in contrast to many biological cargoes that have lipid surfaces that m...

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Autores principales: Bovyn, Matthew, Janakaloti Narayanareddy, Babu Reddy, Gross, Steven, Allard, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108528/
https://www.ncbi.nlm.nih.gov/pubmed/33439674
http://dx.doi.org/10.1091/mbc.E20-10-0658
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author Bovyn, Matthew
Janakaloti Narayanareddy, Babu Reddy
Gross, Steven
Allard, Jun
author_facet Bovyn, Matthew
Janakaloti Narayanareddy, Babu Reddy
Gross, Steven
Allard, Jun
author_sort Bovyn, Matthew
collection PubMed
description Cellular cargoes, including lipid droplets and mitochondria, are transported along microtubules using molecular motors such as kinesins. Many experimental and computational studies focused on cargoes with rigidly attached motors, in contrast to many biological cargoes that have lipid surfaces that may allow surface mobility of motors. We extend a mechanochemical three-dimensional computational model by adding coupled-viscosity effects to compare different motor arrangements and mobilities. We show that organizational changes can optimize for different objectives: Cargoes with clustered motors are transported efficiently but are slow to bind to microtubules, whereas those with motors dispersed rigidly on their surface bind microtubules quickly but are transported inefficiently. Finally, cargoes with freely diffusing motors have both fast binding and efficient transport, although less efficient than clustered motors. These results suggest that experimentally observed changes in motor organization may be a control point for transport.
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spelling pubmed-81085282021-07-04 Diffusion of kinesin motors on cargo can enhance binding and run lengths during intracellular transport Bovyn, Matthew Janakaloti Narayanareddy, Babu Reddy Gross, Steven Allard, Jun Mol Biol Cell Articles Cellular cargoes, including lipid droplets and mitochondria, are transported along microtubules using molecular motors such as kinesins. Many experimental and computational studies focused on cargoes with rigidly attached motors, in contrast to many biological cargoes that have lipid surfaces that may allow surface mobility of motors. We extend a mechanochemical three-dimensional computational model by adding coupled-viscosity effects to compare different motor arrangements and mobilities. We show that organizational changes can optimize for different objectives: Cargoes with clustered motors are transported efficiently but are slow to bind to microtubules, whereas those with motors dispersed rigidly on their surface bind microtubules quickly but are transported inefficiently. Finally, cargoes with freely diffusing motors have both fast binding and efficient transport, although less efficient than clustered motors. These results suggest that experimentally observed changes in motor organization may be a control point for transport. The American Society for Cell Biology 2021-04-19 /pmc/articles/PMC8108528/ /pubmed/33439674 http://dx.doi.org/10.1091/mbc.E20-10-0658 Text en © 2021 Bovyn et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. https://creativecommons.org/licenses/by-nc-sa/3.0/This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License.
spellingShingle Articles
Bovyn, Matthew
Janakaloti Narayanareddy, Babu Reddy
Gross, Steven
Allard, Jun
Diffusion of kinesin motors on cargo can enhance binding and run lengths during intracellular transport
title Diffusion of kinesin motors on cargo can enhance binding and run lengths during intracellular transport
title_full Diffusion of kinesin motors on cargo can enhance binding and run lengths during intracellular transport
title_fullStr Diffusion of kinesin motors on cargo can enhance binding and run lengths during intracellular transport
title_full_unstemmed Diffusion of kinesin motors on cargo can enhance binding and run lengths during intracellular transport
title_short Diffusion of kinesin motors on cargo can enhance binding and run lengths during intracellular transport
title_sort diffusion of kinesin motors on cargo can enhance binding and run lengths during intracellular transport
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8108528/
https://www.ncbi.nlm.nih.gov/pubmed/33439674
http://dx.doi.org/10.1091/mbc.E20-10-0658
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