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Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells

Hepatocellular carcinoma is a common type of liver cancer. Resistance to chemotherapeutic agents is a major problem in cancer therapy. MicroRNAs have been reported in cancer development and tumor growth; however, the relationship between chemoresistance and hepatocellular carcinoma needs to be fully...

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Autores principales: Chen, Hsin-An, Li, Chi-Cheng, Lin, Yu-Jung, Wang, Tso-Fu, Chen, Ming-Cheng, Su, Yen-Hao, Yeh, Yu-Lan, Padma, V. Vijaya, Liao, Po-Hsiang, Huang, Chih-Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8109096/
https://www.ncbi.nlm.nih.gov/pubmed/33901009
http://dx.doi.org/10.18632/aging.202908
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author Chen, Hsin-An
Li, Chi-Cheng
Lin, Yu-Jung
Wang, Tso-Fu
Chen, Ming-Cheng
Su, Yen-Hao
Yeh, Yu-Lan
Padma, V. Vijaya
Liao, Po-Hsiang
Huang, Chih-Yang
author_facet Chen, Hsin-An
Li, Chi-Cheng
Lin, Yu-Jung
Wang, Tso-Fu
Chen, Ming-Cheng
Su, Yen-Hao
Yeh, Yu-Lan
Padma, V. Vijaya
Liao, Po-Hsiang
Huang, Chih-Yang
author_sort Chen, Hsin-An
collection PubMed
description Hepatocellular carcinoma is a common type of liver cancer. Resistance to chemotherapeutic agents is a major problem in cancer therapy. MicroRNAs have been reported in cancer development and tumor growth; however, the relationship between chemoresistance and hepatocellular carcinoma needs to be fully investigated. Here, we treated hepatocellular carcinoma cell line (HA22T) with a histone deacetylase inhibitor to establish hepatocellular carcinoma-resistant cells (HDACi-R) and investigated the molecular mechanisms of chemoresistance in HCC cells. Although histone deacetylase inhibitor could not enhance cell death in HDACi-R but upregulation of miR-107 decreased cell viability both in parental cells and resistance cells, decreased the expression of cofilin-1, enhanced ROS-induced cell apoptosis, and dose-dependently sensitized HDACi-R to HDACi. Further, miR-107 upregulation resulted in tumor cell disorganization in both HA22T and HDACi-R in a mice xenograft model. Our findings demonstrated that miR-107 downregulation leads to hepatocellular carcinoma cell resistance in HDACi via a cofilin-1-dependent molecular mechanism and ROS accumulation.
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spelling pubmed-81090962021-05-12 Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells Chen, Hsin-An Li, Chi-Cheng Lin, Yu-Jung Wang, Tso-Fu Chen, Ming-Cheng Su, Yen-Hao Yeh, Yu-Lan Padma, V. Vijaya Liao, Po-Hsiang Huang, Chih-Yang Aging (Albany NY) Research Paper Hepatocellular carcinoma is a common type of liver cancer. Resistance to chemotherapeutic agents is a major problem in cancer therapy. MicroRNAs have been reported in cancer development and tumor growth; however, the relationship between chemoresistance and hepatocellular carcinoma needs to be fully investigated. Here, we treated hepatocellular carcinoma cell line (HA22T) with a histone deacetylase inhibitor to establish hepatocellular carcinoma-resistant cells (HDACi-R) and investigated the molecular mechanisms of chemoresistance in HCC cells. Although histone deacetylase inhibitor could not enhance cell death in HDACi-R but upregulation of miR-107 decreased cell viability both in parental cells and resistance cells, decreased the expression of cofilin-1, enhanced ROS-induced cell apoptosis, and dose-dependently sensitized HDACi-R to HDACi. Further, miR-107 upregulation resulted in tumor cell disorganization in both HA22T and HDACi-R in a mice xenograft model. Our findings demonstrated that miR-107 downregulation leads to hepatocellular carcinoma cell resistance in HDACi via a cofilin-1-dependent molecular mechanism and ROS accumulation. Impact Journals 2021-04-26 /pmc/articles/PMC8109096/ /pubmed/33901009 http://dx.doi.org/10.18632/aging.202908 Text en Copyright: © 2021 Chen et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Chen, Hsin-An
Li, Chi-Cheng
Lin, Yu-Jung
Wang, Tso-Fu
Chen, Ming-Cheng
Su, Yen-Hao
Yeh, Yu-Lan
Padma, V. Vijaya
Liao, Po-Hsiang
Huang, Chih-Yang
Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells
title Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells
title_full Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells
title_fullStr Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells
title_full_unstemmed Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells
title_short Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells
title_sort hsa-mir-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8109096/
https://www.ncbi.nlm.nih.gov/pubmed/33901009
http://dx.doi.org/10.18632/aging.202908
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