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Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells
Hepatocellular carcinoma is a common type of liver cancer. Resistance to chemotherapeutic agents is a major problem in cancer therapy. MicroRNAs have been reported in cancer development and tumor growth; however, the relationship between chemoresistance and hepatocellular carcinoma needs to be fully...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8109096/ https://www.ncbi.nlm.nih.gov/pubmed/33901009 http://dx.doi.org/10.18632/aging.202908 |
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author | Chen, Hsin-An Li, Chi-Cheng Lin, Yu-Jung Wang, Tso-Fu Chen, Ming-Cheng Su, Yen-Hao Yeh, Yu-Lan Padma, V. Vijaya Liao, Po-Hsiang Huang, Chih-Yang |
author_facet | Chen, Hsin-An Li, Chi-Cheng Lin, Yu-Jung Wang, Tso-Fu Chen, Ming-Cheng Su, Yen-Hao Yeh, Yu-Lan Padma, V. Vijaya Liao, Po-Hsiang Huang, Chih-Yang |
author_sort | Chen, Hsin-An |
collection | PubMed |
description | Hepatocellular carcinoma is a common type of liver cancer. Resistance to chemotherapeutic agents is a major problem in cancer therapy. MicroRNAs have been reported in cancer development and tumor growth; however, the relationship between chemoresistance and hepatocellular carcinoma needs to be fully investigated. Here, we treated hepatocellular carcinoma cell line (HA22T) with a histone deacetylase inhibitor to establish hepatocellular carcinoma-resistant cells (HDACi-R) and investigated the molecular mechanisms of chemoresistance in HCC cells. Although histone deacetylase inhibitor could not enhance cell death in HDACi-R but upregulation of miR-107 decreased cell viability both in parental cells and resistance cells, decreased the expression of cofilin-1, enhanced ROS-induced cell apoptosis, and dose-dependently sensitized HDACi-R to HDACi. Further, miR-107 upregulation resulted in tumor cell disorganization in both HA22T and HDACi-R in a mice xenograft model. Our findings demonstrated that miR-107 downregulation leads to hepatocellular carcinoma cell resistance in HDACi via a cofilin-1-dependent molecular mechanism and ROS accumulation. |
format | Online Article Text |
id | pubmed-8109096 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-81090962021-05-12 Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells Chen, Hsin-An Li, Chi-Cheng Lin, Yu-Jung Wang, Tso-Fu Chen, Ming-Cheng Su, Yen-Hao Yeh, Yu-Lan Padma, V. Vijaya Liao, Po-Hsiang Huang, Chih-Yang Aging (Albany NY) Research Paper Hepatocellular carcinoma is a common type of liver cancer. Resistance to chemotherapeutic agents is a major problem in cancer therapy. MicroRNAs have been reported in cancer development and tumor growth; however, the relationship between chemoresistance and hepatocellular carcinoma needs to be fully investigated. Here, we treated hepatocellular carcinoma cell line (HA22T) with a histone deacetylase inhibitor to establish hepatocellular carcinoma-resistant cells (HDACi-R) and investigated the molecular mechanisms of chemoresistance in HCC cells. Although histone deacetylase inhibitor could not enhance cell death in HDACi-R but upregulation of miR-107 decreased cell viability both in parental cells and resistance cells, decreased the expression of cofilin-1, enhanced ROS-induced cell apoptosis, and dose-dependently sensitized HDACi-R to HDACi. Further, miR-107 upregulation resulted in tumor cell disorganization in both HA22T and HDACi-R in a mice xenograft model. Our findings demonstrated that miR-107 downregulation leads to hepatocellular carcinoma cell resistance in HDACi via a cofilin-1-dependent molecular mechanism and ROS accumulation. Impact Journals 2021-04-26 /pmc/articles/PMC8109096/ /pubmed/33901009 http://dx.doi.org/10.18632/aging.202908 Text en Copyright: © 2021 Chen et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Chen, Hsin-An Li, Chi-Cheng Lin, Yu-Jung Wang, Tso-Fu Chen, Ming-Cheng Su, Yen-Hao Yeh, Yu-Lan Padma, V. Vijaya Liao, Po-Hsiang Huang, Chih-Yang Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells |
title | Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells |
title_full | Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells |
title_fullStr | Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells |
title_full_unstemmed | Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells |
title_short | Hsa-miR-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells |
title_sort | hsa-mir-107 regulates chemosensitivity and inhibits tumor growth in hepatocellular carcinoma cells |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8109096/ https://www.ncbi.nlm.nih.gov/pubmed/33901009 http://dx.doi.org/10.18632/aging.202908 |
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