Cargando…
Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip
Background: Developmental dysplasia of the hip (DDH) is the most common joint disease in child orthopedics. Secreted Frizzled-Related Protein 3 (FRZB) plays an important role in joint development. however, no direct association between FRZB and DDH has been demonstrated. Methods: Analysis of genotyp...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8109121/ https://www.ncbi.nlm.nih.gov/pubmed/33820867 http://dx.doi.org/10.18632/aging.202815 |
_version_ | 1783690199248666624 |
---|---|
author | Xu, Renjie Zhang, Fei Lu, Junlan Wang, Kexin Pan, Peng Sun, Ye Zhang, Yuxin |
author_facet | Xu, Renjie Zhang, Fei Lu, Junlan Wang, Kexin Pan, Peng Sun, Ye Zhang, Yuxin |
author_sort | Xu, Renjie |
collection | PubMed |
description | Background: Developmental dysplasia of the hip (DDH) is the most common joint disease in child orthopedics. Secreted Frizzled-Related Protein 3 (FRZB) plays an important role in joint development. however, no direct association between FRZB and DDH has been demonstrated. Methods: Analysis of genotype distribution and allele frequency for detected single nucleotide polymorphisms (SNP) of FRZB was performed. FRZB expression was assayed in DDH joint tissues. Further experiments to identify the chondrogenic properties of FRZB were conducted. Potential upstream miRNAs for FRZB were assayed in DDH. Results: Significant difference in genotype distribution for rs3768842 (OR=1.46, P=0.0081) and rs2242040 (OR=0.65, P=0.0067) was found. DDH joint tissues showed significantly higher FRZB expression. FRZB demonstrated chondrogenic and anti-hypertrophic properties in vitro. FRZB modulated cell adhesion pathway and cell spreading by regulating integrins expressions. Upstream miRNAs regulating FRZB expression were identified in DDH synovial fluid. Experiments indicated that downregulated miRNA-454 caused FRZB upregulation in DDH joint. Conclusion: Dysregulated FRZB and its loci were associated with DDH. As a Wnt antagonist with chondrogenic properties, FRZB modulated cell adhesion pathway and cell spreading by regulating integrins expressions. FRZB in multiple DDH joint tissues might be mediated by the dysregulated miRNA expression profiles in the joint synovial fluid. |
format | Online Article Text |
id | pubmed-8109121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-81091212021-05-12 Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip Xu, Renjie Zhang, Fei Lu, Junlan Wang, Kexin Pan, Peng Sun, Ye Zhang, Yuxin Aging (Albany NY) Research Paper Background: Developmental dysplasia of the hip (DDH) is the most common joint disease in child orthopedics. Secreted Frizzled-Related Protein 3 (FRZB) plays an important role in joint development. however, no direct association between FRZB and DDH has been demonstrated. Methods: Analysis of genotype distribution and allele frequency for detected single nucleotide polymorphisms (SNP) of FRZB was performed. FRZB expression was assayed in DDH joint tissues. Further experiments to identify the chondrogenic properties of FRZB were conducted. Potential upstream miRNAs for FRZB were assayed in DDH. Results: Significant difference in genotype distribution for rs3768842 (OR=1.46, P=0.0081) and rs2242040 (OR=0.65, P=0.0067) was found. DDH joint tissues showed significantly higher FRZB expression. FRZB demonstrated chondrogenic and anti-hypertrophic properties in vitro. FRZB modulated cell adhesion pathway and cell spreading by regulating integrins expressions. Upstream miRNAs regulating FRZB expression were identified in DDH synovial fluid. Experiments indicated that downregulated miRNA-454 caused FRZB upregulation in DDH joint. Conclusion: Dysregulated FRZB and its loci were associated with DDH. As a Wnt antagonist with chondrogenic properties, FRZB modulated cell adhesion pathway and cell spreading by regulating integrins expressions. FRZB in multiple DDH joint tissues might be mediated by the dysregulated miRNA expression profiles in the joint synovial fluid. Impact Journals 2021-04-04 /pmc/articles/PMC8109121/ /pubmed/33820867 http://dx.doi.org/10.18632/aging.202815 Text en Copyright: © 2021 Xu et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Xu, Renjie Zhang, Fei Lu, Junlan Wang, Kexin Pan, Peng Sun, Ye Zhang, Yuxin Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip |
title | Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip |
title_full | Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip |
title_fullStr | Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip |
title_full_unstemmed | Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip |
title_short | Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip |
title_sort | secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8109121/ https://www.ncbi.nlm.nih.gov/pubmed/33820867 http://dx.doi.org/10.18632/aging.202815 |
work_keys_str_mv | AT xurenjie secretedfrizzledrelatedprotein3wasgeneticallyandfunctionallyassociatedwithdevelopmentaldysplasiaofthehip AT zhangfei secretedfrizzledrelatedprotein3wasgeneticallyandfunctionallyassociatedwithdevelopmentaldysplasiaofthehip AT lujunlan secretedfrizzledrelatedprotein3wasgeneticallyandfunctionallyassociatedwithdevelopmentaldysplasiaofthehip AT wangkexin secretedfrizzledrelatedprotein3wasgeneticallyandfunctionallyassociatedwithdevelopmentaldysplasiaofthehip AT panpeng secretedfrizzledrelatedprotein3wasgeneticallyandfunctionallyassociatedwithdevelopmentaldysplasiaofthehip AT sunye secretedfrizzledrelatedprotein3wasgeneticallyandfunctionallyassociatedwithdevelopmentaldysplasiaofthehip AT zhangyuxin secretedfrizzledrelatedprotein3wasgeneticallyandfunctionallyassociatedwithdevelopmentaldysplasiaofthehip |