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Use of single guided Cas9 nickase to facilitate precise and efficient genome editing in human iPSCs
Cas9 nucleases permit rapid and efficient generation of gene-edited cell lines. However, in typical protocols, mutations are intentionally introduced into the donor template to avoid the cleavage of donor template or re-cleavage of the successfully edited allele, compromising the fidelity of the iso...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Nature Publishing Group UK
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8110799/ https://www.ncbi.nlm.nih.gov/pubmed/33972655 http://dx.doi.org/10.1038/s41598-021-89312-2 |