Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device

Current commercially available methods for reliably detecting antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remain expensive and inaccessible due to the need for whole-blood collection by highly trained phlebotomists using personal protective equipment (PPE). We hav...

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Detalles Bibliográficos
Autores principales: MacMullan, Melanie A., Chellamuthu, Prithivi, Mades, Aubree, Das, Sudipta, Turner, Fred, Slepnev, Vladimir I., Ibrayeva, Albina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Immunoassays
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111129/
https://www.ncbi.nlm.nih.gov/pubmed/33234589
http://dx.doi.org/10.1128/JCM.02510-20
Descripción
Sumario:Current commercially available methods for reliably detecting antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remain expensive and inaccessible due to the need for whole-blood collection by highly trained phlebotomists using personal protective equipment (PPE). We have evaluated an antibody detection approach using the OraSure Technologies oral antibody collection device (OACD) and their proprietary SARS-CoV-2 total antibody detection enzyme-linked immunosorbent assay (ELISA). We found that the OraSure test for total antibody detection in oral fluid had comparable sensitivity and specificity to commercially available serum-based ELISAs for SARS-CoV-2 antibody detection while allowing for a more accessible form of specimen collection with the potential for self-collection.

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