Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device

Current commercially available methods for reliably detecting antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remain expensive and inaccessible due to the need for whole-blood collection by highly trained phlebotomists using personal protective equipment (PPE). We hav...

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Autores principales: MacMullan, Melanie A., Chellamuthu, Prithivi, Mades, Aubree, Das, Sudipta, Turner, Fred, Slepnev, Vladimir I., Ibrayeva, Albina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Immunoassays
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111129/
https://www.ncbi.nlm.nih.gov/pubmed/33234589
http://dx.doi.org/10.1128/JCM.02510-20
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author MacMullan, Melanie A.
Chellamuthu, Prithivi
Mades, Aubree
Das, Sudipta
Turner, Fred
Slepnev, Vladimir I.
Ibrayeva, Albina
author_facet MacMullan, Melanie A.
Chellamuthu, Prithivi
Mades, Aubree
Das, Sudipta
Turner, Fred
Slepnev, Vladimir I.
Ibrayeva, Albina
author_sort MacMullan, Melanie A.
collection PubMed
description Current commercially available methods for reliably detecting antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remain expensive and inaccessible due to the need for whole-blood collection by highly trained phlebotomists using personal protective equipment (PPE). We have evaluated an antibody detection approach using the OraSure Technologies oral antibody collection device (OACD) and their proprietary SARS-CoV-2 total antibody detection enzyme-linked immunosorbent assay (ELISA). We found that the OraSure test for total antibody detection in oral fluid had comparable sensitivity and specificity to commercially available serum-based ELISAs for SARS-CoV-2 antibody detection while allowing for a more accessible form of specimen collection with the potential for self-collection.
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spelling pubmed-81111292021-05-28 Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device MacMullan, Melanie A. Chellamuthu, Prithivi Mades, Aubree Das, Sudipta Turner, Fred Slepnev, Vladimir I. Ibrayeva, Albina J Clin Microbiol Immunoassays Current commercially available methods for reliably detecting antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) remain expensive and inaccessible due to the need for whole-blood collection by highly trained phlebotomists using personal protective equipment (PPE). We have evaluated an antibody detection approach using the OraSure Technologies oral antibody collection device (OACD) and their proprietary SARS-CoV-2 total antibody detection enzyme-linked immunosorbent assay (ELISA). We found that the OraSure test for total antibody detection in oral fluid had comparable sensitivity and specificity to commercially available serum-based ELISAs for SARS-CoV-2 antibody detection while allowing for a more accessible form of specimen collection with the potential for self-collection. American Society for Microbiology 2021-01-21 /pmc/articles/PMC8111129/ /pubmed/33234589 http://dx.doi.org/10.1128/JCM.02510-20 Text en Copyright © 2021 American Society for Microbiology. https://doi.org/10.1128/ASMCopyrightv2All Rights Reserved (https://doi.org/10.1128/ASMCopyrightv2) .
spellingShingle Immunoassays
MacMullan, Melanie A.
Chellamuthu, Prithivi
Mades, Aubree
Das, Sudipta
Turner, Fred
Slepnev, Vladimir I.
Ibrayeva, Albina
Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device
title Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device
title_full Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device
title_fullStr Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device
title_full_unstemmed Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device
title_short Detection of SARS-CoV-2 Antibodies in Oral Fluid Obtained Using a Rapid Collection Device
title_sort detection of sars-cov-2 antibodies in oral fluid obtained using a rapid collection device
topic Immunoassays
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111129/
https://www.ncbi.nlm.nih.gov/pubmed/33234589
http://dx.doi.org/10.1128/JCM.02510-20
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