Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens

This study aimed to evaluate the sampling method for the detection of Ascaris suum larval DNA in chicken livers using real-time PCR. Chickens were inoculated with A. suum eggs of a single dose (Group A) or repeatedly low doses (Group B). White spots (WSs) were continuously observed on liver from day...

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Autores principales: NGUYEN, Yen Thi Hoang, NONAKA, Nariaki, MARUYAMA, Haruhiko, YOSHIDA, Ayako
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2021
Materias:
Parasitology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111357/
https://www.ncbi.nlm.nih.gov/pubmed/33658451
http://dx.doi.org/10.1292/jvms.20-0404
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author NGUYEN, Yen Thi Hoang
NONAKA, Nariaki
MARUYAMA, Haruhiko
YOSHIDA, Ayako
author_facet NGUYEN, Yen Thi Hoang
NONAKA, Nariaki
MARUYAMA, Haruhiko
YOSHIDA, Ayako
author_sort NGUYEN, Yen Thi Hoang
collection PubMed
description This study aimed to evaluate the sampling method for the detection of Ascaris suum larval DNA in chicken livers using real-time PCR. Chickens were inoculated with A. suum eggs of a single dose (Group A) or repeatedly low doses (Group B). White spots (WSs) were continuously observed on liver from day 3 after the last infection in Group B and day 14 in Group A. In Group A, larval DNA was detected in WS lesions (78.6%) at a significantly higher rate than in the remaining tissue samples (31.3%). In conclusion, applying WS lesions to the assay improved the detection rate of A. suum DNA in chicken livers, especially in the case of a single infection.
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spelling pubmed-81113572021-05-13 Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens NGUYEN, Yen Thi Hoang NONAKA, Nariaki MARUYAMA, Haruhiko YOSHIDA, Ayako J Vet Med Sci Parasitology This study aimed to evaluate the sampling method for the detection of Ascaris suum larval DNA in chicken livers using real-time PCR. Chickens were inoculated with A. suum eggs of a single dose (Group A) or repeatedly low doses (Group B). White spots (WSs) were continuously observed on liver from day 3 after the last infection in Group B and day 14 in Group A. In Group A, larval DNA was detected in WS lesions (78.6%) at a significantly higher rate than in the remaining tissue samples (31.3%). In conclusion, applying WS lesions to the assay improved the detection rate of A. suum DNA in chicken livers, especially in the case of a single infection. The Japanese Society of Veterinary Science 2021-03-04 2021-04 /pmc/articles/PMC8111357/ /pubmed/33658451 http://dx.doi.org/10.1292/jvms.20-0404 Text en ©2021 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Parasitology
NGUYEN, Yen Thi Hoang
NONAKA, Nariaki
MARUYAMA, Haruhiko
YOSHIDA, Ayako
Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens
title Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens
title_full Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens
title_fullStr Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens
title_full_unstemmed Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens
title_short Application of a real-time PCR assay for the detection of Ascaris suum DNA in the liver of experimentally infected chickens
title_sort application of a real-time pcr assay for the detection of ascaris suum dna in the liver of experimentally infected chickens
topic Parasitology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111357/
https://www.ncbi.nlm.nih.gov/pubmed/33658451
http://dx.doi.org/10.1292/jvms.20-0404
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