Molecular characterization of Ascaridia galli from Bangladesh and development of a PCR method for distinguishing A. galli from Heterakis spp.

We analyzed the nuclear ribosomal internal transcribed spacer (ITS) 1 and ITS2 sequences for Bangladesh isolates of Ascaridia galli, and we determined that the sequences were unreliable as molecular markers for distinguishing A. galli from other Ascaridia species, because the sequences showed high i...

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Autores principales: BISWAS, Peru Gopal, OHARI, Yuma, MOHANTA, Uday Kumar, ITAGAKI, Tadashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2021
Materias:
Parasitology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111360/
https://www.ncbi.nlm.nih.gov/pubmed/33612660
http://dx.doi.org/10.1292/jvms.20-0728
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author BISWAS, Peru Gopal
OHARI, Yuma
MOHANTA, Uday Kumar
ITAGAKI, Tadashi
author_facet BISWAS, Peru Gopal
OHARI, Yuma
MOHANTA, Uday Kumar
ITAGAKI, Tadashi
author_sort BISWAS, Peru Gopal
collection PubMed
description We analyzed the nuclear ribosomal internal transcribed spacer (ITS) 1 and ITS2 sequences for Bangladesh isolates of Ascaridia galli, and we determined that the sequences were unreliable as molecular markers for distinguishing A. galli from other Ascaridia species, because the sequences showed high identity with that of A. columbae. However, the ITS1 sequences were available for designing PCR primers distinguishable between Ascaridia galli and Heterakis spp. Bangladesh isolates of A. galli constituted a monophyletic clade along with other geographical isolates in the cytochrome c oxidase subunit I (COI) phylogenetic tree, however, we could not clarify the phylogenetic relationships between A. galli and other Ascaridia spp., because their available sequences in GenBank were very few. The developed PCR method using DNA from A. galli and Heterakis spp. eggs would enable differential diagnosis of the individual infections in the future.
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spelling pubmed-81113602021-05-13 Molecular characterization of Ascaridia galli from Bangladesh and development of a PCR method for distinguishing A. galli from Heterakis spp. BISWAS, Peru Gopal OHARI, Yuma MOHANTA, Uday Kumar ITAGAKI, Tadashi J Vet Med Sci Parasitology We analyzed the nuclear ribosomal internal transcribed spacer (ITS) 1 and ITS2 sequences for Bangladesh isolates of Ascaridia galli, and we determined that the sequences were unreliable as molecular markers for distinguishing A. galli from other Ascaridia species, because the sequences showed high identity with that of A. columbae. However, the ITS1 sequences were available for designing PCR primers distinguishable between Ascaridia galli and Heterakis spp. Bangladesh isolates of A. galli constituted a monophyletic clade along with other geographical isolates in the cytochrome c oxidase subunit I (COI) phylogenetic tree, however, we could not clarify the phylogenetic relationships between A. galli and other Ascaridia spp., because their available sequences in GenBank were very few. The developed PCR method using DNA from A. galli and Heterakis spp. eggs would enable differential diagnosis of the individual infections in the future. The Japanese Society of Veterinary Science 2021-02-22 2021-04 /pmc/articles/PMC8111360/ /pubmed/33612660 http://dx.doi.org/10.1292/jvms.20-0728 Text en ©2021 The Japanese Society of Veterinary Science https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Parasitology
BISWAS, Peru Gopal
OHARI, Yuma
MOHANTA, Uday Kumar
ITAGAKI, Tadashi
Molecular characterization of Ascaridia galli from Bangladesh and development of a PCR method for distinguishing A. galli from Heterakis spp.
title Molecular characterization of Ascaridia galli from Bangladesh and development of a PCR method for distinguishing A. galli from Heterakis spp.
title_full Molecular characterization of Ascaridia galli from Bangladesh and development of a PCR method for distinguishing A. galli from Heterakis spp.
title_fullStr Molecular characterization of Ascaridia galli from Bangladesh and development of a PCR method for distinguishing A. galli from Heterakis spp.
title_full_unstemmed Molecular characterization of Ascaridia galli from Bangladesh and development of a PCR method for distinguishing A. galli from Heterakis spp.
title_short Molecular characterization of Ascaridia galli from Bangladesh and development of a PCR method for distinguishing A. galli from Heterakis spp.
title_sort molecular characterization of ascaridia galli from bangladesh and development of a pcr method for distinguishing a. galli from heterakis spp.
topic Parasitology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111360/
https://www.ncbi.nlm.nih.gov/pubmed/33612660
http://dx.doi.org/10.1292/jvms.20-0728
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