tRNA-derived fragments as novel potential biomarkers for relapsed/refractory multiple myeloma

BACKGROUND: tRNA-derived fragments have been reported to be key regulatory factors in human tumors. However, their roles in the progression of multiple myeloma remain unknown. RESULTS: This study employed RNA-sequencing to explore the expression profiles of tRFs/tiRNAs in new diagnosed MM and relaps...

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Autores principales: Xu, Cong, Liang, Ting, Zhang, Fangrong, Liu, Jing, Fu, Yunfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111751/
https://www.ncbi.nlm.nih.gov/pubmed/33971811
http://dx.doi.org/10.1186/s12859-021-04167-8
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author Xu, Cong
Liang, Ting
Zhang, Fangrong
Liu, Jing
Fu, Yunfeng
author_facet Xu, Cong
Liang, Ting
Zhang, Fangrong
Liu, Jing
Fu, Yunfeng
author_sort Xu, Cong
collection PubMed
description BACKGROUND: tRNA-derived fragments have been reported to be key regulatory factors in human tumors. However, their roles in the progression of multiple myeloma remain unknown. RESULTS: This study employed RNA-sequencing to explore the expression profiles of tRFs/tiRNAs in new diagnosed MM and relapsed/refractory MM samples. The expression of selected tRFs/tiRNAs were further validated in clinical specimens and myeloma cell lines by qPCR. Bioinformatic analysis was performed to predict their roles in multiple myeloma progression.We identified 10 upregulated tRFs/tiRNAs and 16 downregulated tRFs/tiRNAs. GO enrichment and KEGG pathway analysis were performed to analyse the functions of 1 significantly up-regulated and 1 significantly down-regulated tRNA-derived fragments. tRFs/tiRNAs may be involved in MM progression and drug-resistance. CONCLUSION: tRFs/tiRNAs were dysregulated and could be potential biomarkers for relapsed/refractory MM. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12859-021-04167-8.
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spelling pubmed-81117512021-05-11 tRNA-derived fragments as novel potential biomarkers for relapsed/refractory multiple myeloma Xu, Cong Liang, Ting Zhang, Fangrong Liu, Jing Fu, Yunfeng BMC Bioinformatics Research BACKGROUND: tRNA-derived fragments have been reported to be key regulatory factors in human tumors. However, their roles in the progression of multiple myeloma remain unknown. RESULTS: This study employed RNA-sequencing to explore the expression profiles of tRFs/tiRNAs in new diagnosed MM and relapsed/refractory MM samples. The expression of selected tRFs/tiRNAs were further validated in clinical specimens and myeloma cell lines by qPCR. Bioinformatic analysis was performed to predict their roles in multiple myeloma progression.We identified 10 upregulated tRFs/tiRNAs and 16 downregulated tRFs/tiRNAs. GO enrichment and KEGG pathway analysis were performed to analyse the functions of 1 significantly up-regulated and 1 significantly down-regulated tRNA-derived fragments. tRFs/tiRNAs may be involved in MM progression and drug-resistance. CONCLUSION: tRFs/tiRNAs were dysregulated and could be potential biomarkers for relapsed/refractory MM. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12859-021-04167-8. BioMed Central 2021-05-11 /pmc/articles/PMC8111751/ /pubmed/33971811 http://dx.doi.org/10.1186/s12859-021-04167-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Xu, Cong
Liang, Ting
Zhang, Fangrong
Liu, Jing
Fu, Yunfeng
tRNA-derived fragments as novel potential biomarkers for relapsed/refractory multiple myeloma
title tRNA-derived fragments as novel potential biomarkers for relapsed/refractory multiple myeloma
title_full tRNA-derived fragments as novel potential biomarkers for relapsed/refractory multiple myeloma
title_fullStr tRNA-derived fragments as novel potential biomarkers for relapsed/refractory multiple myeloma
title_full_unstemmed tRNA-derived fragments as novel potential biomarkers for relapsed/refractory multiple myeloma
title_short tRNA-derived fragments as novel potential biomarkers for relapsed/refractory multiple myeloma
title_sort trna-derived fragments as novel potential biomarkers for relapsed/refractory multiple myeloma
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111751/
https://www.ncbi.nlm.nih.gov/pubmed/33971811
http://dx.doi.org/10.1186/s12859-021-04167-8
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