Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver

Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct phenotypes and functional adaptations that are dependent on their physiological environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to low flow rates. Isolating high-purity EC...

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Detalles Bibliográficos
Autores principales: Sokol, Liliana, Geldhof, Vincent, García-Caballero, Melissa, Conchinha, Nadine V., Dumas, Sébastien J., Meta, Elda, Teuwen, Laure-Anne, Veys, Koen, Chen, Rongyuan, Treps, Lucas, Borri, Mila, de Zeeuw, Pauline, Falkenberg, Kim D., Dubois, Charlotte, Parys, Magdalena, de Rooij, Laura P.M.H., Goveia, Jermaine, Rohlenova, Katerina, Schoonjans, Luc, Dewerchin, Mieke, Eelen, Guy, Li, Xuri, Kalucka, Joanna, Carmeliet, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111824/
https://www.ncbi.nlm.nih.gov/pubmed/34007969
http://dx.doi.org/10.1016/j.xpro.2021.100489
Descripción
Sumario:Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct phenotypes and functional adaptations that are dependent on their physiological environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to low flow rates. Isolating high-purity ECs in sufficient quantities is crucial to study their functions. Here, we describe protocols combining magnetic and fluorescent activated cell sorting for rapid and reproducible EC purification from four adult murine tissues. For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020).

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