Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver

Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct phenotypes and functional adaptations that are dependent on their physiological environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to low flow rates. Isolating high-purity EC...

Descripción completa

Detalles Bibliográficos
Autores principales: Sokol, Liliana, Geldhof, Vincent, García-Caballero, Melissa, Conchinha, Nadine V., Dumas, Sébastien J., Meta, Elda, Teuwen, Laure-Anne, Veys, Koen, Chen, Rongyuan, Treps, Lucas, Borri, Mila, de Zeeuw, Pauline, Falkenberg, Kim D., Dubois, Charlotte, Parys, Magdalena, de Rooij, Laura P.M.H., Goveia, Jermaine, Rohlenova, Katerina, Schoonjans, Luc, Dewerchin, Mieke, Eelen, Guy, Li, Xuri, Kalucka, Joanna, Carmeliet, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111824/
https://www.ncbi.nlm.nih.gov/pubmed/34007969
http://dx.doi.org/10.1016/j.xpro.2021.100489
_version_ 1783690575355052032
author Sokol, Liliana
Geldhof, Vincent
García-Caballero, Melissa
Conchinha, Nadine V.
Dumas, Sébastien J.
Meta, Elda
Teuwen, Laure-Anne
Veys, Koen
Chen, Rongyuan
Treps, Lucas
Borri, Mila
de Zeeuw, Pauline
Falkenberg, Kim D.
Dubois, Charlotte
Parys, Magdalena
de Rooij, Laura P.M.H.
Goveia, Jermaine
Rohlenova, Katerina
Schoonjans, Luc
Dewerchin, Mieke
Eelen, Guy
Li, Xuri
Kalucka, Joanna
Carmeliet, Peter
author_facet Sokol, Liliana
Geldhof, Vincent
García-Caballero, Melissa
Conchinha, Nadine V.
Dumas, Sébastien J.
Meta, Elda
Teuwen, Laure-Anne
Veys, Koen
Chen, Rongyuan
Treps, Lucas
Borri, Mila
de Zeeuw, Pauline
Falkenberg, Kim D.
Dubois, Charlotte
Parys, Magdalena
de Rooij, Laura P.M.H.
Goveia, Jermaine
Rohlenova, Katerina
Schoonjans, Luc
Dewerchin, Mieke
Eelen, Guy
Li, Xuri
Kalucka, Joanna
Carmeliet, Peter
author_sort Sokol, Liliana
collection PubMed
description Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct phenotypes and functional adaptations that are dependent on their physiological environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to low flow rates. Isolating high-purity ECs in sufficient quantities is crucial to study their functions. Here, we describe protocols combining magnetic and fluorescent activated cell sorting for rapid and reproducible EC purification from four adult murine tissues. For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020).
format Online
Article
Text
id pubmed-8111824
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-81118242021-05-17 Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver Sokol, Liliana Geldhof, Vincent García-Caballero, Melissa Conchinha, Nadine V. Dumas, Sébastien J. Meta, Elda Teuwen, Laure-Anne Veys, Koen Chen, Rongyuan Treps, Lucas Borri, Mila de Zeeuw, Pauline Falkenberg, Kim D. Dubois, Charlotte Parys, Magdalena de Rooij, Laura P.M.H. Goveia, Jermaine Rohlenova, Katerina Schoonjans, Luc Dewerchin, Mieke Eelen, Guy Li, Xuri Kalucka, Joanna Carmeliet, Peter STAR Protoc Protocol Endothelial cells (ECs) from the small intestine, colon, liver, and heart have distinct phenotypes and functional adaptations that are dependent on their physiological environment. Gut ECs adapt to low oxygen, heart ECs to contractile forces, and liver ECs to low flow rates. Isolating high-purity ECs in sufficient quantities is crucial to study their functions. Here, we describe protocols combining magnetic and fluorescent activated cell sorting for rapid and reproducible EC purification from four adult murine tissues. For complete details on the use and execution of these protocols, please refer to Kalucka et al. (2020). Elsevier 2021-05-01 /pmc/articles/PMC8111824/ /pubmed/34007969 http://dx.doi.org/10.1016/j.xpro.2021.100489 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sokol, Liliana
Geldhof, Vincent
García-Caballero, Melissa
Conchinha, Nadine V.
Dumas, Sébastien J.
Meta, Elda
Teuwen, Laure-Anne
Veys, Koen
Chen, Rongyuan
Treps, Lucas
Borri, Mila
de Zeeuw, Pauline
Falkenberg, Kim D.
Dubois, Charlotte
Parys, Magdalena
de Rooij, Laura P.M.H.
Goveia, Jermaine
Rohlenova, Katerina
Schoonjans, Luc
Dewerchin, Mieke
Eelen, Guy
Li, Xuri
Kalucka, Joanna
Carmeliet, Peter
Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver
title Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver
title_full Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver
title_fullStr Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver
title_full_unstemmed Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver
title_short Protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver
title_sort protocols for endothelial cell isolation from mouse tissues: small intestine, colon, heart, and liver
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8111824/
https://www.ncbi.nlm.nih.gov/pubmed/34007969
http://dx.doi.org/10.1016/j.xpro.2021.100489
work_keys_str_mv AT sokolliliana protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT geldhofvincent protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT garciacaballeromelissa protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT conchinhanadinev protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT dumassebastienj protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT metaelda protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT teuwenlaureanne protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT veyskoen protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT chenrongyuan protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT trepslucas protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT borrimila protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT dezeeuwpauline protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT falkenbergkimd protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT duboischarlotte protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT parysmagdalena protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT derooijlaurapmh protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT goveiajermaine protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT rohlenovakaterina protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT schoonjansluc protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT dewerchinmieke protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT eelenguy protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT lixuri protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT kaluckajoanna protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver
AT carmelietpeter protocolsforendothelialcellisolationfrommousetissuessmallintestinecolonheartandliver

Ejemplares similares