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Optical Imaging of Pancreatic Innervation
At the time of Ivan Pavlov, pancreatic innervation was studied by looking at pancreas secretions in response to electrical stimulation of nerves. Nowadays we have ways to visualize neuronal activity in real time thanks to advances in fluorescent reporters and imaging techniques. We also have very pr...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112238/ https://www.ncbi.nlm.nih.gov/pubmed/33986728 http://dx.doi.org/10.3389/fendo.2021.663022 |
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author | Makhmutova, Madina Caicedo, Alejandro |
author_facet | Makhmutova, Madina Caicedo, Alejandro |
author_sort | Makhmutova, Madina |
collection | PubMed |
description | At the time of Ivan Pavlov, pancreatic innervation was studied by looking at pancreas secretions in response to electrical stimulation of nerves. Nowadays we have ways to visualize neuronal activity in real time thanks to advances in fluorescent reporters and imaging techniques. We also have very precise optogenetic and pharmacogenetic approaches that allow neuronal manipulations in a very specific manner. These technological advances have been extensively employed for studying the central nervous system and are just beginning to be incorporated for studying visceral innervation. Pancreatic innervation is complex, and the role it plays in physiology and pathophysiology of the organ is still not fully understood. In this review we highlight anatomical aspects of pancreatic innervation, techniques for pancreatic neuronal labeling, and approaches for imaging pancreatic innervation in vitro and in vivo. |
format | Online Article Text |
id | pubmed-8112238 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81122382021-05-12 Optical Imaging of Pancreatic Innervation Makhmutova, Madina Caicedo, Alejandro Front Endocrinol (Lausanne) Endocrinology At the time of Ivan Pavlov, pancreatic innervation was studied by looking at pancreas secretions in response to electrical stimulation of nerves. Nowadays we have ways to visualize neuronal activity in real time thanks to advances in fluorescent reporters and imaging techniques. We also have very precise optogenetic and pharmacogenetic approaches that allow neuronal manipulations in a very specific manner. These technological advances have been extensively employed for studying the central nervous system and are just beginning to be incorporated for studying visceral innervation. Pancreatic innervation is complex, and the role it plays in physiology and pathophysiology of the organ is still not fully understood. In this review we highlight anatomical aspects of pancreatic innervation, techniques for pancreatic neuronal labeling, and approaches for imaging pancreatic innervation in vitro and in vivo. Frontiers Media S.A. 2021-04-27 /pmc/articles/PMC8112238/ /pubmed/33986728 http://dx.doi.org/10.3389/fendo.2021.663022 Text en Copyright © 2021 Makhmutova and Caicedo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Endocrinology Makhmutova, Madina Caicedo, Alejandro Optical Imaging of Pancreatic Innervation |
title | Optical Imaging of Pancreatic Innervation |
title_full | Optical Imaging of Pancreatic Innervation |
title_fullStr | Optical Imaging of Pancreatic Innervation |
title_full_unstemmed | Optical Imaging of Pancreatic Innervation |
title_short | Optical Imaging of Pancreatic Innervation |
title_sort | optical imaging of pancreatic innervation |
topic | Endocrinology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112238/ https://www.ncbi.nlm.nih.gov/pubmed/33986728 http://dx.doi.org/10.3389/fendo.2021.663022 |
work_keys_str_mv | AT makhmutovamadina opticalimagingofpancreaticinnervation AT caicedoalejandro opticalimagingofpancreaticinnervation |