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Assessment of mismatch repair deficiency, CDX2, beta-catenin and E-cadherin expression in colon cancer: molecular characteristics and impact on prognosis and survival – an immunohistochemical study

Microsatellite instability (MSI) or the deficiency of mismatch repair (MMR) proteins is one of the molecular pathways of colorectal tumorigenesis and may have important clinical implications in predicting the treatment response. We evaluated the relationship between clinicopathological features and...

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Autores principales: Melincovici, Carmen Stanca, Boşca, Adina Bianca, Şuşman, Sergiu, Cutaş, Ancuţa, Mărginean, Mariana, Ilea, Aranka, Moldovan, Ioana Maria, Jianu, Elena Mihaela, Neag, Maria Adriana, Bulboacă, Adriana-Elena, Mihu, Carmen Mihaela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Academy of Medical Sciences, Romanian Academy Publishing House, Bucharest 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112747/
https://www.ncbi.nlm.nih.gov/pubmed/33817713
http://dx.doi.org/10.47162/RJME.61.3.10
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author Melincovici, Carmen Stanca
Boşca, Adina Bianca
Şuşman, Sergiu
Cutaş, Ancuţa
Mărginean, Mariana
Ilea, Aranka
Moldovan, Ioana Maria
Jianu, Elena Mihaela
Neag, Maria Adriana
Bulboacă, Adriana-Elena
Mihu, Carmen Mihaela
author_facet Melincovici, Carmen Stanca
Boşca, Adina Bianca
Şuşman, Sergiu
Cutaş, Ancuţa
Mărginean, Mariana
Ilea, Aranka
Moldovan, Ioana Maria
Jianu, Elena Mihaela
Neag, Maria Adriana
Bulboacă, Adriana-Elena
Mihu, Carmen Mihaela
author_sort Melincovici, Carmen Stanca
collection PubMed
description Microsatellite instability (MSI) or the deficiency of mismatch repair (MMR) proteins is one of the molecular pathways of colorectal tumorigenesis and may have important clinical implications in predicting the treatment response. We evaluated the relationship between clinicopathological features and MMR proteins [mutL homologue 1 (MLH1), mutS homologue 2 (MSH2), mutS homologue 6 (MSH6), postmeiotic segregation increased 2 (PMS2)], adhesion molecules (E-cadherin, beta-catenin) and caudal-type homeobox 2 (CDX2) in 31 patients with colon adenocarcinoma, using immunohistochemistry. We also aimed to assess the prognostic value of the studied proteins. MLH1 loss was correlated to PMS2 loss (p=0.006) and MSH2 loss (p=0.023); MSH2 loss was significantly associated to MSH6 loss (p=0.011). Tumors with MSH6 loss, together with tumors with PMS2 loss, covered all the patients with MSI status. We found a significant correlation between MSI tumors and mucinous histological type (p=0.03), but no significant associations with other clinicopathological features or with survival rate. There was a significant correlation between E-cadherin expression and differentiation degree (p=0.018) and between beta-catenin expression and lymph node invasion (p=0.046). No significant association between CDX2 loss and any clinical or pathological features was found (p>0.05). No significant differences were identified in overall survival according to E-cadherin, beta-catenin or CDX2 expression (p>0.05). In our study, PMS2 loss was significantly correlated with CDX2 loss (p=0.03). In conclusion, the molecular analysis of biological markers for colon cancer may be important for patient stratification, in order to select the optimal treatment algorithm. Our results suggest that probably the double panel (MSH6 and PMS2) is enough to detect the MSI status, instead of using the quadruple panel.
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spelling pubmed-81127472021-06-01 Assessment of mismatch repair deficiency, CDX2, beta-catenin and E-cadherin expression in colon cancer: molecular characteristics and impact on prognosis and survival – an immunohistochemical study Melincovici, Carmen Stanca Boşca, Adina Bianca Şuşman, Sergiu Cutaş, Ancuţa Mărginean, Mariana Ilea, Aranka Moldovan, Ioana Maria Jianu, Elena Mihaela Neag, Maria Adriana Bulboacă, Adriana-Elena Mihu, Carmen Mihaela Rom J Morphol Embryol Original Paper Microsatellite instability (MSI) or the deficiency of mismatch repair (MMR) proteins is one of the molecular pathways of colorectal tumorigenesis and may have important clinical implications in predicting the treatment response. We evaluated the relationship between clinicopathological features and MMR proteins [mutL homologue 1 (MLH1), mutS homologue 2 (MSH2), mutS homologue 6 (MSH6), postmeiotic segregation increased 2 (PMS2)], adhesion molecules (E-cadherin, beta-catenin) and caudal-type homeobox 2 (CDX2) in 31 patients with colon adenocarcinoma, using immunohistochemistry. We also aimed to assess the prognostic value of the studied proteins. MLH1 loss was correlated to PMS2 loss (p=0.006) and MSH2 loss (p=0.023); MSH2 loss was significantly associated to MSH6 loss (p=0.011). Tumors with MSH6 loss, together with tumors with PMS2 loss, covered all the patients with MSI status. We found a significant correlation between MSI tumors and mucinous histological type (p=0.03), but no significant associations with other clinicopathological features or with survival rate. There was a significant correlation between E-cadherin expression and differentiation degree (p=0.018) and between beta-catenin expression and lymph node invasion (p=0.046). No significant association between CDX2 loss and any clinical or pathological features was found (p>0.05). No significant differences were identified in overall survival according to E-cadherin, beta-catenin or CDX2 expression (p>0.05). In our study, PMS2 loss was significantly correlated with CDX2 loss (p=0.03). In conclusion, the molecular analysis of biological markers for colon cancer may be important for patient stratification, in order to select the optimal treatment algorithm. Our results suggest that probably the double panel (MSH6 and PMS2) is enough to detect the MSI status, instead of using the quadruple panel. Academy of Medical Sciences, Romanian Academy Publishing House, Bucharest 2020 2021-03-28 /pmc/articles/PMC8112747/ /pubmed/33817713 http://dx.doi.org/10.47162/RJME.61.3.10 Text en Copyright © 2020, Academy of Medical Sciences, Romanian Academy Publishing House, Bucharest https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open-access article distributed under the terms of a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International Public License, which permits unrestricted use, adaptation, distribution and reproduction in any medium, non-commercially, provided the new creations are licensed under identical terms as the original work and the original work is properly cited.
spellingShingle Original Paper
Melincovici, Carmen Stanca
Boşca, Adina Bianca
Şuşman, Sergiu
Cutaş, Ancuţa
Mărginean, Mariana
Ilea, Aranka
Moldovan, Ioana Maria
Jianu, Elena Mihaela
Neag, Maria Adriana
Bulboacă, Adriana-Elena
Mihu, Carmen Mihaela
Assessment of mismatch repair deficiency, CDX2, beta-catenin and E-cadherin expression in colon cancer: molecular characteristics and impact on prognosis and survival – an immunohistochemical study
title Assessment of mismatch repair deficiency, CDX2, beta-catenin and E-cadherin expression in colon cancer: molecular characteristics and impact on prognosis and survival – an immunohistochemical study
title_full Assessment of mismatch repair deficiency, CDX2, beta-catenin and E-cadherin expression in colon cancer: molecular characteristics and impact on prognosis and survival – an immunohistochemical study
title_fullStr Assessment of mismatch repair deficiency, CDX2, beta-catenin and E-cadherin expression in colon cancer: molecular characteristics and impact on prognosis and survival – an immunohistochemical study
title_full_unstemmed Assessment of mismatch repair deficiency, CDX2, beta-catenin and E-cadherin expression in colon cancer: molecular characteristics and impact on prognosis and survival – an immunohistochemical study
title_short Assessment of mismatch repair deficiency, CDX2, beta-catenin and E-cadherin expression in colon cancer: molecular characteristics and impact on prognosis and survival – an immunohistochemical study
title_sort assessment of mismatch repair deficiency, cdx2, beta-catenin and e-cadherin expression in colon cancer: molecular characteristics and impact on prognosis and survival – an immunohistochemical study
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112747/
https://www.ncbi.nlm.nih.gov/pubmed/33817713
http://dx.doi.org/10.47162/RJME.61.3.10
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