Differences in the Expression of KIR, ILT Inhibitory Receptors, and VEGF Production in the Induced Decidual NK Cell Cultures of Fertile and RPL Women

RESULTS: KIR2DL1 and ILT-2 expression on idNK cells was higher in healthy women than in RPL patients. Sildenafil enhanced NKG2A expression in RPL patients. VEGF concentration was higher in fertile woman idNK cell cultures. idNK cells were more sensitive for necrosis in RPL than in fertile women. SC...

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Autores principales: Kniotek, Monika, Roszczyk, Aleksander, Zych, Michał, Szafarowska, Monika, Jerzak, Małgorzata
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112925/
https://www.ncbi.nlm.nih.gov/pubmed/33997038
http://dx.doi.org/10.1155/2021/6673427
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author Kniotek, Monika
Roszczyk, Aleksander
Zych, Michał
Szafarowska, Monika
Jerzak, Małgorzata
author_facet Kniotek, Monika
Roszczyk, Aleksander
Zych, Michał
Szafarowska, Monika
Jerzak, Małgorzata
author_sort Kniotek, Monika
collection PubMed
description RESULTS: KIR2DL1 and ILT-2 expression on idNK cells was higher in healthy women than in RPL patients. Sildenafil enhanced NKG2A expression in RPL patients. VEGF concentration was higher in fertile woman idNK cell cultures. idNK cells were more sensitive for necrosis in RPL than in fertile women. SC did not influence VEGF production or idNK cell apoptosis. CONCLUSIONS: A combination of hypoxia, IL-15, and AZA promotes the conversion of pbNK into idNK cells CD56(+)CD16(−)-expressing KIR receptors and produces VEGF. Alterations in KIR2DL1 and ILT-2 expression as well as impaired VEGF production were associated with RPL. SC affects NKG2A expression on RPL idNK cells. SC had no effect on VEGF release or idNK cell apoptosis.
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spelling pubmed-81129252021-05-13 Differences in the Expression of KIR, ILT Inhibitory Receptors, and VEGF Production in the Induced Decidual NK Cell Cultures of Fertile and RPL Women Kniotek, Monika Roszczyk, Aleksander Zych, Michał Szafarowska, Monika Jerzak, Małgorzata Biomed Res Int Research Article RESULTS: KIR2DL1 and ILT-2 expression on idNK cells was higher in healthy women than in RPL patients. Sildenafil enhanced NKG2A expression in RPL patients. VEGF concentration was higher in fertile woman idNK cell cultures. idNK cells were more sensitive for necrosis in RPL than in fertile women. SC did not influence VEGF production or idNK cell apoptosis. CONCLUSIONS: A combination of hypoxia, IL-15, and AZA promotes the conversion of pbNK into idNK cells CD56(+)CD16(−)-expressing KIR receptors and produces VEGF. Alterations in KIR2DL1 and ILT-2 expression as well as impaired VEGF production were associated with RPL. SC affects NKG2A expression on RPL idNK cells. SC had no effect on VEGF release or idNK cell apoptosis. Hindawi 2021-05-04 /pmc/articles/PMC8112925/ /pubmed/33997038 http://dx.doi.org/10.1155/2021/6673427 Text en Copyright © 2021 Monika Kniotek et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Kniotek, Monika
Roszczyk, Aleksander
Zych, Michał
Szafarowska, Monika
Jerzak, Małgorzata
Differences in the Expression of KIR, ILT Inhibitory Receptors, and VEGF Production in the Induced Decidual NK Cell Cultures of Fertile and RPL Women
title Differences in the Expression of KIR, ILT Inhibitory Receptors, and VEGF Production in the Induced Decidual NK Cell Cultures of Fertile and RPL Women
title_full Differences in the Expression of KIR, ILT Inhibitory Receptors, and VEGF Production in the Induced Decidual NK Cell Cultures of Fertile and RPL Women
title_fullStr Differences in the Expression of KIR, ILT Inhibitory Receptors, and VEGF Production in the Induced Decidual NK Cell Cultures of Fertile and RPL Women
title_full_unstemmed Differences in the Expression of KIR, ILT Inhibitory Receptors, and VEGF Production in the Induced Decidual NK Cell Cultures of Fertile and RPL Women
title_short Differences in the Expression of KIR, ILT Inhibitory Receptors, and VEGF Production in the Induced Decidual NK Cell Cultures of Fertile and RPL Women
title_sort differences in the expression of kir, ilt inhibitory receptors, and vegf production in the induced decidual nk cell cultures of fertile and rpl women
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8112925/
https://www.ncbi.nlm.nih.gov/pubmed/33997038
http://dx.doi.org/10.1155/2021/6673427
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