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Echinacea purpurea Ethanol Extract Improves Male Reproductive Dysfunction With Streptozotocin–Nicotinamide-Induced Diabetic Rats

As lifestyle changes, the prevalence of diabetes increases every year. Diabetes-induced male reproductive dysfunction is predominantly due to increased oxidative stress and then results in sperm damage and infertility. Echinacea purpurea is a traditional medicinal herb and is well-known for its immu...

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Autores principales: Mao, Chien-Feng, Sudirman, Sabri, Lee, Chi-Chih, Tsou, David, Kong, Zwe-Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113381/
https://www.ncbi.nlm.nih.gov/pubmed/33996978
http://dx.doi.org/10.3389/fvets.2021.651286
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author Mao, Chien-Feng
Sudirman, Sabri
Lee, Chi-Chih
Tsou, David
Kong, Zwe-Ling
author_facet Mao, Chien-Feng
Sudirman, Sabri
Lee, Chi-Chih
Tsou, David
Kong, Zwe-Ling
author_sort Mao, Chien-Feng
collection PubMed
description As lifestyle changes, the prevalence of diabetes increases every year. Diabetes-induced male reproductive dysfunction is predominantly due to increased oxidative stress and then results in sperm damage and infertility. Echinacea purpurea is a traditional medicinal herb and is well-known for its immune-modulatory, antioxidative, anti-inflammatory, anticancer, and antiviral activities. The Toll-like receptor 4 (TLR4) plays a critical role in innate immune responses leading to nuclear factor (NF)-κB phosphorylation and release of proinflammatory cytokines including nitric oxide (NO), interleukin (IL)-1β, and tumor necrosis factor (TNF)-α. However, the relation between Echinacea purpurea extract and TLR4 remains unclear. This study aimed to investigate the protective effects on male reproduction of Echinacea purpurea ethanol extract (EPE) against diabetic rats and whether the anti-inflammatory effects were through the TLR4 pathway. Diabetic male Sprague–Dawley (SD) rats were induced by streptozotocin (65 mg/kg) and nicotinamide (230 mg/kg). EPE was tested in three doses (93, 279, and 465 mg/kg p.o. daily) for 4 weeks. Besides, metformin administration (100 mg/kg/day) was treated as a positive control. Results indicated that EPE administration for about 4 weeks improved hyperglycemia and insulin resistance. Additionally, EPE increased sperm motility, protected sperm morphology and mitochondrial membrane potential, as well as protein for testosterone synthesis enzyme. In sperm superoxide dismutase, catalase, and glutathione antioxidants were increased, whereas proinflammatory cytokines, such as NO, IL-1β, and TNF-α were decreased. The testis protein content of TLR4 and downstream phospho-NF-κB p65 also were reduced. The EPE might reduce the production of proinflammatory cytokines via TLR4 pathways and improve diabetes-induced male infertility.
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spelling pubmed-81133812021-05-13 Echinacea purpurea Ethanol Extract Improves Male Reproductive Dysfunction With Streptozotocin–Nicotinamide-Induced Diabetic Rats Mao, Chien-Feng Sudirman, Sabri Lee, Chi-Chih Tsou, David Kong, Zwe-Ling Front Vet Sci Veterinary Science As lifestyle changes, the prevalence of diabetes increases every year. Diabetes-induced male reproductive dysfunction is predominantly due to increased oxidative stress and then results in sperm damage and infertility. Echinacea purpurea is a traditional medicinal herb and is well-known for its immune-modulatory, antioxidative, anti-inflammatory, anticancer, and antiviral activities. The Toll-like receptor 4 (TLR4) plays a critical role in innate immune responses leading to nuclear factor (NF)-κB phosphorylation and release of proinflammatory cytokines including nitric oxide (NO), interleukin (IL)-1β, and tumor necrosis factor (TNF)-α. However, the relation between Echinacea purpurea extract and TLR4 remains unclear. This study aimed to investigate the protective effects on male reproduction of Echinacea purpurea ethanol extract (EPE) against diabetic rats and whether the anti-inflammatory effects were through the TLR4 pathway. Diabetic male Sprague–Dawley (SD) rats were induced by streptozotocin (65 mg/kg) and nicotinamide (230 mg/kg). EPE was tested in three doses (93, 279, and 465 mg/kg p.o. daily) for 4 weeks. Besides, metformin administration (100 mg/kg/day) was treated as a positive control. Results indicated that EPE administration for about 4 weeks improved hyperglycemia and insulin resistance. Additionally, EPE increased sperm motility, protected sperm morphology and mitochondrial membrane potential, as well as protein for testosterone synthesis enzyme. In sperm superoxide dismutase, catalase, and glutathione antioxidants were increased, whereas proinflammatory cytokines, such as NO, IL-1β, and TNF-α were decreased. The testis protein content of TLR4 and downstream phospho-NF-κB p65 also were reduced. The EPE might reduce the production of proinflammatory cytokines via TLR4 pathways and improve diabetes-induced male infertility. Frontiers Media S.A. 2021-04-28 /pmc/articles/PMC8113381/ /pubmed/33996978 http://dx.doi.org/10.3389/fvets.2021.651286 Text en Copyright © 2021 Mao, Sudirman, Lee, Tsou and Kong. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Mao, Chien-Feng
Sudirman, Sabri
Lee, Chi-Chih
Tsou, David
Kong, Zwe-Ling
Echinacea purpurea Ethanol Extract Improves Male Reproductive Dysfunction With Streptozotocin–Nicotinamide-Induced Diabetic Rats
title Echinacea purpurea Ethanol Extract Improves Male Reproductive Dysfunction With Streptozotocin–Nicotinamide-Induced Diabetic Rats
title_full Echinacea purpurea Ethanol Extract Improves Male Reproductive Dysfunction With Streptozotocin–Nicotinamide-Induced Diabetic Rats
title_fullStr Echinacea purpurea Ethanol Extract Improves Male Reproductive Dysfunction With Streptozotocin–Nicotinamide-Induced Diabetic Rats
title_full_unstemmed Echinacea purpurea Ethanol Extract Improves Male Reproductive Dysfunction With Streptozotocin–Nicotinamide-Induced Diabetic Rats
title_short Echinacea purpurea Ethanol Extract Improves Male Reproductive Dysfunction With Streptozotocin–Nicotinamide-Induced Diabetic Rats
title_sort echinacea purpurea ethanol extract improves male reproductive dysfunction with streptozotocin–nicotinamide-induced diabetic rats
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113381/
https://www.ncbi.nlm.nih.gov/pubmed/33996978
http://dx.doi.org/10.3389/fvets.2021.651286
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