Facile isolation and analysis of sporopollenin exine from bee pollen

We present facile methods to obtain purified sporopollenin exine capsules, and provide mass balances for classical and novel purification procedures. An ionic liquid, tetrabutyl phosphonium hydroxide turned out to be the most effective in removing the intine wall. The sporopollenin capsules were inv...

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Autores principales: Hegedüs, Kristóf, Fehér, Csaba, Jalsovszky, István, Kristóf, Zoltán, Rohonczy, János, Vass, Elemér, Farkas, Attila, Csizmadia, Tamás, Friedbacher, Gernot, Hantz, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113464/
https://www.ncbi.nlm.nih.gov/pubmed/33976296
http://dx.doi.org/10.1038/s41598-021-87619-8
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author Hegedüs, Kristóf
Fehér, Csaba
Jalsovszky, István
Kristóf, Zoltán
Rohonczy, János
Vass, Elemér
Farkas, Attila
Csizmadia, Tamás
Friedbacher, Gernot
Hantz, Peter
author_facet Hegedüs, Kristóf
Fehér, Csaba
Jalsovszky, István
Kristóf, Zoltán
Rohonczy, János
Vass, Elemér
Farkas, Attila
Csizmadia, Tamás
Friedbacher, Gernot
Hantz, Peter
author_sort Hegedüs, Kristóf
collection PubMed
description We present facile methods to obtain purified sporopollenin exine capsules, and provide mass balances for classical and novel purification procedures. An ionic liquid, tetrabutyl phosphonium hydroxide turned out to be the most effective in removing the intine wall. The sporopollenin capsules were investigated by fluorescent microscopy, AFM, solid-state NMR and infrared Raman spectroscopy. The latter two methods showed that sunflower and rape exines have different proportions of O-aliphatic and aromatic constituents. Purified exine capsules were coated with functionalized fluorophores. The procedures presented in this paper could contribute to further spread of the applications of this hollow, and chemically highly resistant material.
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spelling pubmed-81134642021-05-12 Facile isolation and analysis of sporopollenin exine from bee pollen Hegedüs, Kristóf Fehér, Csaba Jalsovszky, István Kristóf, Zoltán Rohonczy, János Vass, Elemér Farkas, Attila Csizmadia, Tamás Friedbacher, Gernot Hantz, Peter Sci Rep Article We present facile methods to obtain purified sporopollenin exine capsules, and provide mass balances for classical and novel purification procedures. An ionic liquid, tetrabutyl phosphonium hydroxide turned out to be the most effective in removing the intine wall. The sporopollenin capsules were investigated by fluorescent microscopy, AFM, solid-state NMR and infrared Raman spectroscopy. The latter two methods showed that sunflower and rape exines have different proportions of O-aliphatic and aromatic constituents. Purified exine capsules were coated with functionalized fluorophores. The procedures presented in this paper could contribute to further spread of the applications of this hollow, and chemically highly resistant material. Nature Publishing Group UK 2021-05-11 /pmc/articles/PMC8113464/ /pubmed/33976296 http://dx.doi.org/10.1038/s41598-021-87619-8 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Hegedüs, Kristóf
Fehér, Csaba
Jalsovszky, István
Kristóf, Zoltán
Rohonczy, János
Vass, Elemér
Farkas, Attila
Csizmadia, Tamás
Friedbacher, Gernot
Hantz, Peter
Facile isolation and analysis of sporopollenin exine from bee pollen
title Facile isolation and analysis of sporopollenin exine from bee pollen
title_full Facile isolation and analysis of sporopollenin exine from bee pollen
title_fullStr Facile isolation and analysis of sporopollenin exine from bee pollen
title_full_unstemmed Facile isolation and analysis of sporopollenin exine from bee pollen
title_short Facile isolation and analysis of sporopollenin exine from bee pollen
title_sort facile isolation and analysis of sporopollenin exine from bee pollen
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113464/
https://www.ncbi.nlm.nih.gov/pubmed/33976296
http://dx.doi.org/10.1038/s41598-021-87619-8
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