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JRAB/MICAL-L2 undergoes liquid–liquid phase separation to form tubular recycling endosomes
Elongated tubular endosomes play essential roles in diverse cellular functions. Multiple molecules have been implicated in tubulation of recycling endosomes, but the mechanism of endosomal tubule biogenesis has remained unclear. In this study, we found that JRAB/MICAL-L2 induces endosomal tubulation...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113518/ https://www.ncbi.nlm.nih.gov/pubmed/33976349 http://dx.doi.org/10.1038/s42003-021-02080-7 |
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author | Sakane, Ayuko Yano, Taka-aki Uchihashi, Takayuki Horikawa, Kazuki Hara, Yusuke Imoto, Issei Kurisu, Shusaku Yamada, Hiroshi Takei, Kohji Sasaki, Takuya |
author_facet | Sakane, Ayuko Yano, Taka-aki Uchihashi, Takayuki Horikawa, Kazuki Hara, Yusuke Imoto, Issei Kurisu, Shusaku Yamada, Hiroshi Takei, Kohji Sasaki, Takuya |
author_sort | Sakane, Ayuko |
collection | PubMed |
description | Elongated tubular endosomes play essential roles in diverse cellular functions. Multiple molecules have been implicated in tubulation of recycling endosomes, but the mechanism of endosomal tubule biogenesis has remained unclear. In this study, we found that JRAB/MICAL-L2 induces endosomal tubulation via activated Rab8A. In association with Rab8A, JRAB/MICAL-L2 adopts its closed form, which functions in the tubulation of recycling endosomes. Moreover, JRAB/MICAL-L2 induces liquid–liquid phase separation, initiating the formation of tubular recycling endosomes upon overexpression. Between its N-terminal and C-terminal globular domains, JRAB/MICAL-L2 contains an intrinsically disordered region, which contributes to the formation of JRAB/MICAL-L2 condensates. Based on our findings, we propose that JRAB/MICAL-L2 plays two sequential roles in the biogenesis of tubular recycling endosomes: first, JRAB/MICAL-L2 organizes phase separation, and then the closed form of JRAB/MICAL-L2 formed by interaction with Rab8A promotes endosomal tubulation. |
format | Online Article Text |
id | pubmed-8113518 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-81135182021-05-12 JRAB/MICAL-L2 undergoes liquid–liquid phase separation to form tubular recycling endosomes Sakane, Ayuko Yano, Taka-aki Uchihashi, Takayuki Horikawa, Kazuki Hara, Yusuke Imoto, Issei Kurisu, Shusaku Yamada, Hiroshi Takei, Kohji Sasaki, Takuya Commun Biol Article Elongated tubular endosomes play essential roles in diverse cellular functions. Multiple molecules have been implicated in tubulation of recycling endosomes, but the mechanism of endosomal tubule biogenesis has remained unclear. In this study, we found that JRAB/MICAL-L2 induces endosomal tubulation via activated Rab8A. In association with Rab8A, JRAB/MICAL-L2 adopts its closed form, which functions in the tubulation of recycling endosomes. Moreover, JRAB/MICAL-L2 induces liquid–liquid phase separation, initiating the formation of tubular recycling endosomes upon overexpression. Between its N-terminal and C-terminal globular domains, JRAB/MICAL-L2 contains an intrinsically disordered region, which contributes to the formation of JRAB/MICAL-L2 condensates. Based on our findings, we propose that JRAB/MICAL-L2 plays two sequential roles in the biogenesis of tubular recycling endosomes: first, JRAB/MICAL-L2 organizes phase separation, and then the closed form of JRAB/MICAL-L2 formed by interaction with Rab8A promotes endosomal tubulation. Nature Publishing Group UK 2021-05-11 /pmc/articles/PMC8113518/ /pubmed/33976349 http://dx.doi.org/10.1038/s42003-021-02080-7 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Sakane, Ayuko Yano, Taka-aki Uchihashi, Takayuki Horikawa, Kazuki Hara, Yusuke Imoto, Issei Kurisu, Shusaku Yamada, Hiroshi Takei, Kohji Sasaki, Takuya JRAB/MICAL-L2 undergoes liquid–liquid phase separation to form tubular recycling endosomes |
title | JRAB/MICAL-L2 undergoes liquid–liquid phase separation to form tubular recycling endosomes |
title_full | JRAB/MICAL-L2 undergoes liquid–liquid phase separation to form tubular recycling endosomes |
title_fullStr | JRAB/MICAL-L2 undergoes liquid–liquid phase separation to form tubular recycling endosomes |
title_full_unstemmed | JRAB/MICAL-L2 undergoes liquid–liquid phase separation to form tubular recycling endosomes |
title_short | JRAB/MICAL-L2 undergoes liquid–liquid phase separation to form tubular recycling endosomes |
title_sort | jrab/mical-l2 undergoes liquid–liquid phase separation to form tubular recycling endosomes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113518/ https://www.ncbi.nlm.nih.gov/pubmed/33976349 http://dx.doi.org/10.1038/s42003-021-02080-7 |
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