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Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases

Most functional biopharmaceuticals such as antibodies are glycoproteins carrying N-linked oligosaccharides (N-glycans). In animal cells, these glycans are generally expressed as heterogeneous glycoforms that are difficult to separate into a pure form. The structure of these glycans directly affects...

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Detalles Bibliográficos
Autores principales: Katoh, Toshihiko, Yamamoto, Kenji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Applied Glycoscience 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113915/
https://www.ncbi.nlm.nih.gov/pubmed/34354540
http://dx.doi.org/10.5458/jag.jag.JAG-2020_0013
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author Katoh, Toshihiko
Yamamoto, Kenji
author_facet Katoh, Toshihiko
Yamamoto, Kenji
author_sort Katoh, Toshihiko
collection PubMed
description Most functional biopharmaceuticals such as antibodies are glycoproteins carrying N-linked oligosaccharides (N-glycans). In animal cells, these glycans are generally expressed as heterogeneous glycoforms that are difficult to separate into a pure form. The structure of these glycans directly affects several biological aspects of the glycoproteins, especially binding affinity. Therefore, the preparation of glycoproteins with well-defined and homogeneous glycoforms is necessary for functional studies and improved efficacy, particularly for biopharmaceuticals. This review describes the recent remarkable progress in the development and production of biopharmaceutical glycan-modified antibodies, through the use of glycan remodeling using microbial endoglycosidases and sophisticated glycoengineering techniques utilizing microbial enzymatic reaction mechanisms.
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spelling pubmed-81139152021-08-04 Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases Katoh, Toshihiko Yamamoto, Kenji J Appl Glycosci (1999) Regular Paper Most functional biopharmaceuticals such as antibodies are glycoproteins carrying N-linked oligosaccharides (N-glycans). In animal cells, these glycans are generally expressed as heterogeneous glycoforms that are difficult to separate into a pure form. The structure of these glycans directly affects several biological aspects of the glycoproteins, especially binding affinity. Therefore, the preparation of glycoproteins with well-defined and homogeneous glycoforms is necessary for functional studies and improved efficacy, particularly for biopharmaceuticals. This review describes the recent remarkable progress in the development and production of biopharmaceutical glycan-modified antibodies, through the use of glycan remodeling using microbial endoglycosidases and sophisticated glycoengineering techniques utilizing microbial enzymatic reaction mechanisms. The Japanese Society of Applied Glycoscience 2021-03-11 /pmc/articles/PMC8113915/ /pubmed/34354540 http://dx.doi.org/10.5458/jag.jag.JAG-2020_0013 Text en 2021 by The Japanese Society of Applied Glycoscience https://creativecommons.org/licenses/by-nc/4.0/This is an open-access paper distributed under the terms of the Creative Commons Attribution Non-Commercial (by-nc) License (CC-BY-NC4.0: https://creativecommons.org/licenses/by-nc/4.0/).
spellingShingle Regular Paper
Katoh, Toshihiko
Yamamoto, Kenji
Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases
title Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases
title_full Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases
title_fullStr Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases
title_full_unstemmed Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases
title_short Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases
title_sort innovative preparation of biopharmaceuticals using transglycosylation activity of microbial endoglycosidases
topic Regular Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113915/
https://www.ncbi.nlm.nih.gov/pubmed/34354540
http://dx.doi.org/10.5458/jag.jag.JAG-2020_0013
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