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Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases
Most functional biopharmaceuticals such as antibodies are glycoproteins carrying N-linked oligosaccharides (N-glycans). In animal cells, these glycans are generally expressed as heterogeneous glycoforms that are difficult to separate into a pure form. The structure of these glycans directly affects...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Applied Glycoscience
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113915/ https://www.ncbi.nlm.nih.gov/pubmed/34354540 http://dx.doi.org/10.5458/jag.jag.JAG-2020_0013 |
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author | Katoh, Toshihiko Yamamoto, Kenji |
author_facet | Katoh, Toshihiko Yamamoto, Kenji |
author_sort | Katoh, Toshihiko |
collection | PubMed |
description | Most functional biopharmaceuticals such as antibodies are glycoproteins carrying N-linked oligosaccharides (N-glycans). In animal cells, these glycans are generally expressed as heterogeneous glycoforms that are difficult to separate into a pure form. The structure of these glycans directly affects several biological aspects of the glycoproteins, especially binding affinity. Therefore, the preparation of glycoproteins with well-defined and homogeneous glycoforms is necessary for functional studies and improved efficacy, particularly for biopharmaceuticals. This review describes the recent remarkable progress in the development and production of biopharmaceutical glycan-modified antibodies, through the use of glycan remodeling using microbial endoglycosidases and sophisticated glycoengineering techniques utilizing microbial enzymatic reaction mechanisms. |
format | Online Article Text |
id | pubmed-8113915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Japanese Society of Applied Glycoscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-81139152021-08-04 Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases Katoh, Toshihiko Yamamoto, Kenji J Appl Glycosci (1999) Regular Paper Most functional biopharmaceuticals such as antibodies are glycoproteins carrying N-linked oligosaccharides (N-glycans). In animal cells, these glycans are generally expressed as heterogeneous glycoforms that are difficult to separate into a pure form. The structure of these glycans directly affects several biological aspects of the glycoproteins, especially binding affinity. Therefore, the preparation of glycoproteins with well-defined and homogeneous glycoforms is necessary for functional studies and improved efficacy, particularly for biopharmaceuticals. This review describes the recent remarkable progress in the development and production of biopharmaceutical glycan-modified antibodies, through the use of glycan remodeling using microbial endoglycosidases and sophisticated glycoengineering techniques utilizing microbial enzymatic reaction mechanisms. The Japanese Society of Applied Glycoscience 2021-03-11 /pmc/articles/PMC8113915/ /pubmed/34354540 http://dx.doi.org/10.5458/jag.jag.JAG-2020_0013 Text en 2021 by The Japanese Society of Applied Glycoscience https://creativecommons.org/licenses/by-nc/4.0/This is an open-access paper distributed under the terms of the Creative Commons Attribution Non-Commercial (by-nc) License (CC-BY-NC4.0: https://creativecommons.org/licenses/by-nc/4.0/). |
spellingShingle | Regular Paper Katoh, Toshihiko Yamamoto, Kenji Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases |
title | Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases |
title_full | Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases |
title_fullStr | Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases |
title_full_unstemmed | Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases |
title_short | Innovative Preparation of Biopharmaceuticals Using Transglycosylation Activity of Microbial Endoglycosidases |
title_sort | innovative preparation of biopharmaceuticals using transglycosylation activity of microbial endoglycosidases |
topic | Regular Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113915/ https://www.ncbi.nlm.nih.gov/pubmed/34354540 http://dx.doi.org/10.5458/jag.jag.JAG-2020_0013 |
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