Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro

Immunomodulatory drugs can alter lymphocyte function. Hydroxychloroquine (HCQ) is prescribed for many autoimmune diseases and is under investigation as an anti-tumor autophagy inhibitor. Here, we describe a protocol to evaluate the influence of HCQ on lymphocyte function by measuring the in vitro an...

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Autores principales: Wabitsch, Simon, McCallen, Justin D., Ma, Chi, Greten, Tim F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113974/
https://www.ncbi.nlm.nih.gov/pubmed/34013212
http://dx.doi.org/10.1016/j.xpro.2021.100517
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author Wabitsch, Simon
McCallen, Justin D.
Ma, Chi
Greten, Tim F.
author_facet Wabitsch, Simon
McCallen, Justin D.
Ma, Chi
Greten, Tim F.
author_sort Wabitsch, Simon
collection PubMed
description Immunomodulatory drugs can alter lymphocyte function. Hydroxychloroquine (HCQ) is prescribed for many autoimmune diseases and is under investigation as an anti-tumor autophagy inhibitor. Here, we describe a protocol to evaluate the influence of HCQ on lymphocyte function by measuring the in vitro and ex vivo proliferation and cytokine production. The protocol can provide insights into potential immunomodulatory effects of HCQ and can be used for assessing other medications' effects on lymphocyte functions. For complete details on the use and execution of this protocol, please refer to Wabitsch et al. (2021).
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spelling pubmed-81139742021-05-18 Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro Wabitsch, Simon McCallen, Justin D. Ma, Chi Greten, Tim F. STAR Protoc Protocol Immunomodulatory drugs can alter lymphocyte function. Hydroxychloroquine (HCQ) is prescribed for many autoimmune diseases and is under investigation as an anti-tumor autophagy inhibitor. Here, we describe a protocol to evaluate the influence of HCQ on lymphocyte function by measuring the in vitro and ex vivo proliferation and cytokine production. The protocol can provide insights into potential immunomodulatory effects of HCQ and can be used for assessing other medications' effects on lymphocyte functions. For complete details on the use and execution of this protocol, please refer to Wabitsch et al. (2021). Elsevier 2021-05-03 /pmc/articles/PMC8113974/ /pubmed/34013212 http://dx.doi.org/10.1016/j.xpro.2021.100517 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Wabitsch, Simon
McCallen, Justin D.
Ma, Chi
Greten, Tim F.
Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro
title Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro
title_full Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro
title_fullStr Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro
title_full_unstemmed Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro
title_short Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro
title_sort evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113974/
https://www.ncbi.nlm.nih.gov/pubmed/34013212
http://dx.doi.org/10.1016/j.xpro.2021.100517
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