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Targeted in utero electroporation of the ventro-temporal mouse retina
Techniques enabling DNA delivery into mouse retinal cells using in utero electroporation are available. However, these techniques target the central retina and do not enable the electroporation of the ventro-temporal retina where ipsilateral retinal ganglion cells are located. Here, we describe a pr...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113976/ https://www.ncbi.nlm.nih.gov/pubmed/34013211 http://dx.doi.org/10.1016/j.xpro.2021.100516 |
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author | Louail, Alice Assali, Ahlem Nicol, Xavier |
author_facet | Louail, Alice Assali, Ahlem Nicol, Xavier |
author_sort | Louail, Alice |
collection | PubMed |
description | Techniques enabling DNA delivery into mouse retinal cells using in utero electroporation are available. However, these techniques target the central retina and do not enable the electroporation of the ventro-temporal retina where ipsilateral retinal ganglion cells are located. Here, we describe a protocol to specifically electroporate the ventro-temporal retina, a critical approach to manipulate ipsilaterally projecting retinal ganglion cells and contralaterally projecting neurons located in the same region of the retina. The procedure is adaptable to target other retinal quadrants. For complete details on the use and execution of this protocol, please refer to Louail et al. (2020). |
format | Online Article Text |
id | pubmed-8113976 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-81139762021-05-18 Targeted in utero electroporation of the ventro-temporal mouse retina Louail, Alice Assali, Ahlem Nicol, Xavier STAR Protoc Protocol Techniques enabling DNA delivery into mouse retinal cells using in utero electroporation are available. However, these techniques target the central retina and do not enable the electroporation of the ventro-temporal retina where ipsilateral retinal ganglion cells are located. Here, we describe a protocol to specifically electroporate the ventro-temporal retina, a critical approach to manipulate ipsilaterally projecting retinal ganglion cells and contralaterally projecting neurons located in the same region of the retina. The procedure is adaptable to target other retinal quadrants. For complete details on the use and execution of this protocol, please refer to Louail et al. (2020). Elsevier 2021-05-03 /pmc/articles/PMC8113976/ /pubmed/34013211 http://dx.doi.org/10.1016/j.xpro.2021.100516 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Louail, Alice Assali, Ahlem Nicol, Xavier Targeted in utero electroporation of the ventro-temporal mouse retina |
title | Targeted in utero electroporation of the ventro-temporal mouse retina |
title_full | Targeted in utero electroporation of the ventro-temporal mouse retina |
title_fullStr | Targeted in utero electroporation of the ventro-temporal mouse retina |
title_full_unstemmed | Targeted in utero electroporation of the ventro-temporal mouse retina |
title_short | Targeted in utero electroporation of the ventro-temporal mouse retina |
title_sort | targeted in utero electroporation of the ventro-temporal mouse retina |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113976/ https://www.ncbi.nlm.nih.gov/pubmed/34013211 http://dx.doi.org/10.1016/j.xpro.2021.100516 |
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