Chromatin immunoprecipitation of transcription factors and histone modifications in Comma-Dβ mammary epithelial cells

Chromatin immunoprecipitation (ChIP) is used to study interactions between proteins and DNA. Nuclear lysates are prepared, and chromatin is fragmented by sonication. Antibodies are used to purify a protein of interest (e.g., a transcription factor or histone mark) along with any bound DNA. The genom...

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Detalles Bibliográficos
Autores principales: Holliday, Holly, Khoury, Amanda, Swarbrick, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8113982/
https://www.ncbi.nlm.nih.gov/pubmed/34013210
http://dx.doi.org/10.1016/j.xpro.2021.100514

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