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Anacardium Occidentale L. Leaf Extracts Protect Against Glutamate/H(2)O(2)-Induced Oxidative Toxicity and Induce Neurite Outgrowth: The Involvement of SIRT1/Nrf2 Signaling Pathway and Teneurin 4 Transmembrane Protein
Neurodegenerative diseases are linked to neuronal cell death and neurite outgrowth impairment that are often caused by oxidative stress. Natural products, which have neuroprotective against oxidative stress and neurite outgrowth inducing activity, could be potential candidates for alternative treatm...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114061/ https://www.ncbi.nlm.nih.gov/pubmed/33995025 http://dx.doi.org/10.3389/fphar.2021.627738 |
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author | Duangjan, Chatrawee Rangsinth, Panthakarn Zhang, Shaoxiong Wink, Michael Tencomnao, Tewin |
author_facet | Duangjan, Chatrawee Rangsinth, Panthakarn Zhang, Shaoxiong Wink, Michael Tencomnao, Tewin |
author_sort | Duangjan, Chatrawee |
collection | PubMed |
description | Neurodegenerative diseases are linked to neuronal cell death and neurite outgrowth impairment that are often caused by oxidative stress. Natural products, which have neuroprotective against oxidative stress and neurite outgrowth inducing activity, could be potential candidates for alternative treatment of neurodegenerative diseases. This study aims to investigate the neuroprotective effects and neuritogenesis properties of Anacardium occidentale leaf extracts in cultured neuronal (HT22 and Neuro-2a) cells. We found gallic acid, catechin and quercetin as the main compounds in A. occidentale extracts. The extracts have a protective effect against glutamate/H(2)O(2)-mediated oxidative stress-induced cell toxicity. The gene expression of cellular antioxidant enzymes (SODs, GPx and, GSTs) were up-regulated by this treatment. The treatment also triggered SIRT, Nrf2 proteins as well as the mRNA transcriptions of relevant anti-oxidation genes (NQO1, GCLM, and EAAT3). We demonstrated that the extracts promote antioxidant defense in neuronal cells via the SIRT1/Nrf2 signaling pathway. Moreover, the extracts increase neurite outgrowth and Ten-4 expression in Neuro-2a cells. However, the neuritogenesis properties did not occur, when Ten-4 expression was knocked down by corresponding siRNA. These results suggest that the leaf extracts have an interesting neuritogenesis and neuroprotective potential against glutamate/H(2)O(2)-mediated toxicity and could be a potential therapeutic candidate for neurodegenerative diseases. |
format | Online Article Text |
id | pubmed-8114061 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-81140612021-05-13 Anacardium Occidentale L. Leaf Extracts Protect Against Glutamate/H(2)O(2)-Induced Oxidative Toxicity and Induce Neurite Outgrowth: The Involvement of SIRT1/Nrf2 Signaling Pathway and Teneurin 4 Transmembrane Protein Duangjan, Chatrawee Rangsinth, Panthakarn Zhang, Shaoxiong Wink, Michael Tencomnao, Tewin Front Pharmacol Pharmacology Neurodegenerative diseases are linked to neuronal cell death and neurite outgrowth impairment that are often caused by oxidative stress. Natural products, which have neuroprotective against oxidative stress and neurite outgrowth inducing activity, could be potential candidates for alternative treatment of neurodegenerative diseases. This study aims to investigate the neuroprotective effects and neuritogenesis properties of Anacardium occidentale leaf extracts in cultured neuronal (HT22 and Neuro-2a) cells. We found gallic acid, catechin and quercetin as the main compounds in A. occidentale extracts. The extracts have a protective effect against glutamate/H(2)O(2)-mediated oxidative stress-induced cell toxicity. The gene expression of cellular antioxidant enzymes (SODs, GPx and, GSTs) were up-regulated by this treatment. The treatment also triggered SIRT, Nrf2 proteins as well as the mRNA transcriptions of relevant anti-oxidation genes (NQO1, GCLM, and EAAT3). We demonstrated that the extracts promote antioxidant defense in neuronal cells via the SIRT1/Nrf2 signaling pathway. Moreover, the extracts increase neurite outgrowth and Ten-4 expression in Neuro-2a cells. However, the neuritogenesis properties did not occur, when Ten-4 expression was knocked down by corresponding siRNA. These results suggest that the leaf extracts have an interesting neuritogenesis and neuroprotective potential against glutamate/H(2)O(2)-mediated toxicity and could be a potential therapeutic candidate for neurodegenerative diseases. Frontiers Media S.A. 2021-04-23 /pmc/articles/PMC8114061/ /pubmed/33995025 http://dx.doi.org/10.3389/fphar.2021.627738 Text en Copyright © 2021 Duangjan, Rangsinth, Zhang, Wink and Tencomnao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Duangjan, Chatrawee Rangsinth, Panthakarn Zhang, Shaoxiong Wink, Michael Tencomnao, Tewin Anacardium Occidentale L. Leaf Extracts Protect Against Glutamate/H(2)O(2)-Induced Oxidative Toxicity and Induce Neurite Outgrowth: The Involvement of SIRT1/Nrf2 Signaling Pathway and Teneurin 4 Transmembrane Protein |
title | Anacardium Occidentale L. Leaf Extracts Protect Against Glutamate/H(2)O(2)-Induced Oxidative Toxicity and Induce Neurite Outgrowth: The Involvement of SIRT1/Nrf2 Signaling Pathway and Teneurin 4 Transmembrane Protein |
title_full | Anacardium Occidentale L. Leaf Extracts Protect Against Glutamate/H(2)O(2)-Induced Oxidative Toxicity and Induce Neurite Outgrowth: The Involvement of SIRT1/Nrf2 Signaling Pathway and Teneurin 4 Transmembrane Protein |
title_fullStr | Anacardium Occidentale L. Leaf Extracts Protect Against Glutamate/H(2)O(2)-Induced Oxidative Toxicity and Induce Neurite Outgrowth: The Involvement of SIRT1/Nrf2 Signaling Pathway and Teneurin 4 Transmembrane Protein |
title_full_unstemmed | Anacardium Occidentale L. Leaf Extracts Protect Against Glutamate/H(2)O(2)-Induced Oxidative Toxicity and Induce Neurite Outgrowth: The Involvement of SIRT1/Nrf2 Signaling Pathway and Teneurin 4 Transmembrane Protein |
title_short | Anacardium Occidentale L. Leaf Extracts Protect Against Glutamate/H(2)O(2)-Induced Oxidative Toxicity and Induce Neurite Outgrowth: The Involvement of SIRT1/Nrf2 Signaling Pathway and Teneurin 4 Transmembrane Protein |
title_sort | anacardium occidentale l. leaf extracts protect against glutamate/h(2)o(2)-induced oxidative toxicity and induce neurite outgrowth: the involvement of sirt1/nrf2 signaling pathway and teneurin 4 transmembrane protein |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114061/ https://www.ncbi.nlm.nih.gov/pubmed/33995025 http://dx.doi.org/10.3389/fphar.2021.627738 |
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