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Predicting the Refractive Index of Tissue Models Using Light Scattering Spectroscopy

In this work, we report the application of Raman microspectroscopy for analysis of the refractive index of a range of tissue phantoms. Using both a custom-developed setup with visible laser source and a commercial microspectrometer with near infrared laser, we measured the Raman spectra of gelatin h...

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Detalles Bibliográficos
Autores principales: Bailey, Michelle, Gardner, Benjamin, Alunni-Cardinali, Martina, Caponi, Silvia, Fioretto, Daniele, Stone, Nick, Palombo, Francesca
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114435/
https://www.ncbi.nlm.nih.gov/pubmed/33319606
http://dx.doi.org/10.1177/0003702820984482
Descripción
Sumario:In this work, we report the application of Raman microspectroscopy for analysis of the refractive index of a range of tissue phantoms. Using both a custom-developed setup with visible laser source and a commercial microspectrometer with near infrared laser, we measured the Raman spectra of gelatin hydrogels at various concentrations. By building a calibration curve from measured refractometry data and Raman scattering intensity for different vibrational modes of the hydrogel, we were able to predict the refractive indices of the gels from their Raman spectra. This work highlights the importance of a correlative approach through Brillouin–Raman microspectroscopy for the mechano–chemical analysis of biologically relevant samples.