Cargando…
Thermostable Cellulase Biosynthesis from Paenibacillus alvei and its Utilization in Lactic Acid Production by Simultaneous Saccharification and Fermentation
Cellulosic date palm wastes may have beneficial biotechnological applications for eco-friendly utilization. This study reports the isolation of thermophilic cellulase-producing bacteria and their application in lactic acid production using date palm leaves. The promising isolate was identified as Pa...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
De Gruyter
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114780/ https://www.ncbi.nlm.nih.gov/pubmed/33987475 http://dx.doi.org/10.1515/biol-2020-0019 |
_version_ | 1783691117491912704 |
---|---|
author | Mostafa, Yasser S. Alamri, Saad A. Hashem, Mohamed Nafady, Nivien A. Abo-Elyousr, Kamal A.M. Mohamed, Zakaria A. |
author_facet | Mostafa, Yasser S. Alamri, Saad A. Hashem, Mohamed Nafady, Nivien A. Abo-Elyousr, Kamal A.M. Mohamed, Zakaria A. |
author_sort | Mostafa, Yasser S. |
collection | PubMed |
description | Cellulosic date palm wastes may have beneficial biotechnological applications for eco-friendly utilization. This study reports the isolation of thermophilic cellulase-producing bacteria and their application in lactic acid production using date palm leaves. The promising isolate was identified as Paenibacillus alvei by 16S rRNA gene sequencing. Maximum cellulase production was acquired using alkaline treated date palm leaves (ATDPL) at 48 h and yielded 4.50 U.mL(-1) FPase, 8.11 U.mL(-1) CMCase, and 2.74 U.mL(-1) β-glucosidase. The cellulase activity was optimal at pH 5.0 and 50°C with good stability at a wide temperature (40-70°C) and pH (4.0-7.0) range, demonstrating its suitability in simultaneous saccharification and fermentation. Lactic acid fermentation was optimized at 4 days, pH 5.0, 50°C, 6.0% cellulose of ATDPL, 30 FPU/ g cellulose, 1.0 g. L(-1) Tween 80, and 5.0 g. L(-l) yeast extract using Lactobacillus delbrueckii. The conversion efficiency of lactic acid from the cellulose of ATDPL was 98.71%, and the lactic acid productivity was 0.719 g. L(-1) h(-1). Alkaline treatment exhibited a valuable effect on the production of cellulases and lactic acid by reducing the lignin content and cellulose crystallinity. The results of this study offer a credible procedure for using date palm leaves for microbial industrial applications. |
format | Online Article Text |
id | pubmed-8114780 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | De Gruyter |
record_format | MEDLINE/PubMed |
spelling | pubmed-81147802021-05-12 Thermostable Cellulase Biosynthesis from Paenibacillus alvei and its Utilization in Lactic Acid Production by Simultaneous Saccharification and Fermentation Mostafa, Yasser S. Alamri, Saad A. Hashem, Mohamed Nafady, Nivien A. Abo-Elyousr, Kamal A.M. Mohamed, Zakaria A. Open Life Sci Research Article Cellulosic date palm wastes may have beneficial biotechnological applications for eco-friendly utilization. This study reports the isolation of thermophilic cellulase-producing bacteria and their application in lactic acid production using date palm leaves. The promising isolate was identified as Paenibacillus alvei by 16S rRNA gene sequencing. Maximum cellulase production was acquired using alkaline treated date palm leaves (ATDPL) at 48 h and yielded 4.50 U.mL(-1) FPase, 8.11 U.mL(-1) CMCase, and 2.74 U.mL(-1) β-glucosidase. The cellulase activity was optimal at pH 5.0 and 50°C with good stability at a wide temperature (40-70°C) and pH (4.0-7.0) range, demonstrating its suitability in simultaneous saccharification and fermentation. Lactic acid fermentation was optimized at 4 days, pH 5.0, 50°C, 6.0% cellulose of ATDPL, 30 FPU/ g cellulose, 1.0 g. L(-1) Tween 80, and 5.0 g. L(-l) yeast extract using Lactobacillus delbrueckii. The conversion efficiency of lactic acid from the cellulose of ATDPL was 98.71%, and the lactic acid productivity was 0.719 g. L(-1) h(-1). Alkaline treatment exhibited a valuable effect on the production of cellulases and lactic acid by reducing the lignin content and cellulose crystallinity. The results of this study offer a credible procedure for using date palm leaves for microbial industrial applications. De Gruyter 2020-04-10 /pmc/articles/PMC8114780/ /pubmed/33987475 http://dx.doi.org/10.1515/biol-2020-0019 Text en © 2020 Yasser S. Mostafa, et al. published by De Gruyter https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 Public License. |
spellingShingle | Research Article Mostafa, Yasser S. Alamri, Saad A. Hashem, Mohamed Nafady, Nivien A. Abo-Elyousr, Kamal A.M. Mohamed, Zakaria A. Thermostable Cellulase Biosynthesis from Paenibacillus alvei and its Utilization in Lactic Acid Production by Simultaneous Saccharification and Fermentation |
title | Thermostable Cellulase Biosynthesis from Paenibacillus alvei and its Utilization in Lactic Acid Production by Simultaneous Saccharification and Fermentation |
title_full | Thermostable Cellulase Biosynthesis from Paenibacillus alvei and its Utilization in Lactic Acid Production by Simultaneous Saccharification and Fermentation |
title_fullStr | Thermostable Cellulase Biosynthesis from Paenibacillus alvei and its Utilization in Lactic Acid Production by Simultaneous Saccharification and Fermentation |
title_full_unstemmed | Thermostable Cellulase Biosynthesis from Paenibacillus alvei and its Utilization in Lactic Acid Production by Simultaneous Saccharification and Fermentation |
title_short | Thermostable Cellulase Biosynthesis from Paenibacillus alvei and its Utilization in Lactic Acid Production by Simultaneous Saccharification and Fermentation |
title_sort | thermostable cellulase biosynthesis from paenibacillus alvei and its utilization in lactic acid production by simultaneous saccharification and fermentation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114780/ https://www.ncbi.nlm.nih.gov/pubmed/33987475 http://dx.doi.org/10.1515/biol-2020-0019 |
work_keys_str_mv | AT mostafayassers thermostablecellulasebiosynthesisfrompaenibacillusalveianditsutilizationinlacticacidproductionbysimultaneoussaccharificationandfermentation AT alamrisaada thermostablecellulasebiosynthesisfrompaenibacillusalveianditsutilizationinlacticacidproductionbysimultaneoussaccharificationandfermentation AT hashemmohamed thermostablecellulasebiosynthesisfrompaenibacillusalveianditsutilizationinlacticacidproductionbysimultaneoussaccharificationandfermentation AT nafadyniviena thermostablecellulasebiosynthesisfrompaenibacillusalveianditsutilizationinlacticacidproductionbysimultaneoussaccharificationandfermentation AT aboelyousrkamalam thermostablecellulasebiosynthesisfrompaenibacillusalveianditsutilizationinlacticacidproductionbysimultaneoussaccharificationandfermentation AT mohamedzakariaa thermostablecellulasebiosynthesisfrompaenibacillusalveianditsutilizationinlacticacidproductionbysimultaneoussaccharificationandfermentation |