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The Thickness of Surface Grafting Layer on Bio-materials directly Mediates the Immuno-reacitivity of Macrophages in vitro

Introducing the surface grafting layers to regulate bio-compatibility and bio-function is an important step in the tissue engineering field. However, whether the thickness variation of the introduced biomimetic layer impacts the behavior of the adhered immune effector cells is yet to be dissected fu...

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Detalles Bibliográficos
Autores principales: Xiao, JiangWei, Huang, Tao, Hu, JiJie, Zan, Fei, Liao, ZhaoHong, Gu, RuiCai, Wu, Gang, Liao, Hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: De Gruyter 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114785/
https://www.ncbi.nlm.nih.gov/pubmed/33987476
http://dx.doi.org/10.1515/biol-2020-0020
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author Xiao, JiangWei
Huang, Tao
Hu, JiJie
Zan, Fei
Liao, ZhaoHong
Gu, RuiCai
Wu, Gang
Liao, Hua
author_facet Xiao, JiangWei
Huang, Tao
Hu, JiJie
Zan, Fei
Liao, ZhaoHong
Gu, RuiCai
Wu, Gang
Liao, Hua
author_sort Xiao, JiangWei
collection PubMed
description Introducing the surface grafting layers to regulate bio-compatibility and bio-function is an important step in the tissue engineering field. However, whether the thickness variation of the introduced biomimetic layer impacts the behavior of the adhered immune effector cells is yet to be dissected fully. In this study, we used a surface-induced atom transfer radical polymerization (SI-ATRP) method to synthetize and graft poly-phenoxyethyl methacrylate (PHEMA) brushes having different lengths on the glass substrates. Primary murine peritoneal macrophages were collected and cultured on the PHEMA brushes and we investigated the influence of polymer brushes having different lengths on macrophages phenotype and function. Our results demonstrated that the thicker brushes (200 nm and 450 nm) are superior to the thinner layers (50 nm) for macrophages survival, proliferation, cell elongation and migration. Moreover, the thicker brushes are more beneficial for macrophage’s activities and functions, presented by the increased production of M1-associated cytokines IL-6 and MCP-1, the elevated cell phagocytosis and the activation molecule F4/80 expression, and the reduced macrophages apoptosis in thicker brushes-sustained macrophages. Our data suggests that the thickness of the substrate grafting layer directly impacts macrophages recruitment and pro-inflammatory function, which is important in determining the intrinsic immuno-compatibilities of the surface modified-biomaterials and mediates material-host interactions in vivo.
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spelling pubmed-81147852021-05-12 The Thickness of Surface Grafting Layer on Bio-materials directly Mediates the Immuno-reacitivity of Macrophages in vitro Xiao, JiangWei Huang, Tao Hu, JiJie Zan, Fei Liao, ZhaoHong Gu, RuiCai Wu, Gang Liao, Hua Open Life Sci Research Article Introducing the surface grafting layers to regulate bio-compatibility and bio-function is an important step in the tissue engineering field. However, whether the thickness variation of the introduced biomimetic layer impacts the behavior of the adhered immune effector cells is yet to be dissected fully. In this study, we used a surface-induced atom transfer radical polymerization (SI-ATRP) method to synthetize and graft poly-phenoxyethyl methacrylate (PHEMA) brushes having different lengths on the glass substrates. Primary murine peritoneal macrophages were collected and cultured on the PHEMA brushes and we investigated the influence of polymer brushes having different lengths on macrophages phenotype and function. Our results demonstrated that the thicker brushes (200 nm and 450 nm) are superior to the thinner layers (50 nm) for macrophages survival, proliferation, cell elongation and migration. Moreover, the thicker brushes are more beneficial for macrophage’s activities and functions, presented by the increased production of M1-associated cytokines IL-6 and MCP-1, the elevated cell phagocytosis and the activation molecule F4/80 expression, and the reduced macrophages apoptosis in thicker brushes-sustained macrophages. Our data suggests that the thickness of the substrate grafting layer directly impacts macrophages recruitment and pro-inflammatory function, which is important in determining the intrinsic immuno-compatibilities of the surface modified-biomaterials and mediates material-host interactions in vivo. De Gruyter 2020-05-03 /pmc/articles/PMC8114785/ /pubmed/33987476 http://dx.doi.org/10.1515/biol-2020-0020 Text en © 2020 JiangWei Xiao, et al. published by De Gruyter https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 Public License.
spellingShingle Research Article
Xiao, JiangWei
Huang, Tao
Hu, JiJie
Zan, Fei
Liao, ZhaoHong
Gu, RuiCai
Wu, Gang
Liao, Hua
The Thickness of Surface Grafting Layer on Bio-materials directly Mediates the Immuno-reacitivity of Macrophages in vitro
title The Thickness of Surface Grafting Layer on Bio-materials directly Mediates the Immuno-reacitivity of Macrophages in vitro
title_full The Thickness of Surface Grafting Layer on Bio-materials directly Mediates the Immuno-reacitivity of Macrophages in vitro
title_fullStr The Thickness of Surface Grafting Layer on Bio-materials directly Mediates the Immuno-reacitivity of Macrophages in vitro
title_full_unstemmed The Thickness of Surface Grafting Layer on Bio-materials directly Mediates the Immuno-reacitivity of Macrophages in vitro
title_short The Thickness of Surface Grafting Layer on Bio-materials directly Mediates the Immuno-reacitivity of Macrophages in vitro
title_sort thickness of surface grafting layer on bio-materials directly mediates the immuno-reacitivity of macrophages in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114785/
https://www.ncbi.nlm.nih.gov/pubmed/33987476
http://dx.doi.org/10.1515/biol-2020-0020
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