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Transcriptomic Analysis of the Mechanisms for Alleviating Psoriatic Dermatitis Using Taodan Granules in an Imiquimod-Induced Psoriasis-like Mouse Model

Taodan granules (TDGs) are clinically efficacious for treating psoriasis, buttheir specific mechanisms of action are unclear. In this study, we determined the concentrations of tanshinone IIA and curcumol using high-performance liquid chromatography (HPLC) to establish quality control parameters for...

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Autores principales: Kuai, Le, Luo, Ying, Qu, Keshen, Ru, Yi, Luo, Yue, Ding, Xiaojie, Xing, Meng, Liu, Liu, Sun, Xiaoying, Li, Xin, Li, Bin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114823/
https://www.ncbi.nlm.nih.gov/pubmed/33995034
http://dx.doi.org/10.3389/fphar.2021.632414
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author Kuai, Le
Luo, Ying
Qu, Keshen
Ru, Yi
Luo, Yue
Ding, Xiaojie
Xing, Meng
Liu, Liu
Sun, Xiaoying
Li, Xin
Li, Bin
author_facet Kuai, Le
Luo, Ying
Qu, Keshen
Ru, Yi
Luo, Yue
Ding, Xiaojie
Xing, Meng
Liu, Liu
Sun, Xiaoying
Li, Xin
Li, Bin
author_sort Kuai, Le
collection PubMed
description Taodan granules (TDGs) are clinically efficacious for treating psoriasis, buttheir specific mechanisms of action are unclear. In this study, we determined the concentrations of tanshinone IIA and curcumol using high-performance liquid chromatography (HPLC) to establish quality control parameters for assessing the mechanism of TDGs in treating psoriasis. Thereafter, a mouse model of psoriasis was treated with TDGs. TDGs attenuated imiquimod-induced typical erythema, scales, and thickening of the back and ear lesions in the psoriatic mouse model. Furthermore, PCNA and Ki67-positive cells were reduced in the epidermis of psoriatic lesions following TDG treatment. Finally, the sequencing results were verified using a multitude of methods, and the mechanism of action of TDGs against psoriasis was found to be via the upregulation of metabolic signaling pathways such as the Gly-Ser-Thr axis, the downregulation of immune and inflammatory pathways, and the decrease in Rac2 and Arhgdib concentrations. Overall, this study clarified the mechanism of TDG treatment for psoriasis and provided evidence for its clinical application.
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spelling pubmed-81148232021-05-13 Transcriptomic Analysis of the Mechanisms for Alleviating Psoriatic Dermatitis Using Taodan Granules in an Imiquimod-Induced Psoriasis-like Mouse Model Kuai, Le Luo, Ying Qu, Keshen Ru, Yi Luo, Yue Ding, Xiaojie Xing, Meng Liu, Liu Sun, Xiaoying Li, Xin Li, Bin Front Pharmacol Pharmacology Taodan granules (TDGs) are clinically efficacious for treating psoriasis, buttheir specific mechanisms of action are unclear. In this study, we determined the concentrations of tanshinone IIA and curcumol using high-performance liquid chromatography (HPLC) to establish quality control parameters for assessing the mechanism of TDGs in treating psoriasis. Thereafter, a mouse model of psoriasis was treated with TDGs. TDGs attenuated imiquimod-induced typical erythema, scales, and thickening of the back and ear lesions in the psoriatic mouse model. Furthermore, PCNA and Ki67-positive cells were reduced in the epidermis of psoriatic lesions following TDG treatment. Finally, the sequencing results were verified using a multitude of methods, and the mechanism of action of TDGs against psoriasis was found to be via the upregulation of metabolic signaling pathways such as the Gly-Ser-Thr axis, the downregulation of immune and inflammatory pathways, and the decrease in Rac2 and Arhgdib concentrations. Overall, this study clarified the mechanism of TDG treatment for psoriasis and provided evidence for its clinical application. Frontiers Media S.A. 2021-04-14 /pmc/articles/PMC8114823/ /pubmed/33995034 http://dx.doi.org/10.3389/fphar.2021.632414 Text en Copyright © 2021 Kuai, Luo, Qu, Ru, Luo, Ding, Xing, Liu, Sun, Li and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Kuai, Le
Luo, Ying
Qu, Keshen
Ru, Yi
Luo, Yue
Ding, Xiaojie
Xing, Meng
Liu, Liu
Sun, Xiaoying
Li, Xin
Li, Bin
Transcriptomic Analysis of the Mechanisms for Alleviating Psoriatic Dermatitis Using Taodan Granules in an Imiquimod-Induced Psoriasis-like Mouse Model
title Transcriptomic Analysis of the Mechanisms for Alleviating Psoriatic Dermatitis Using Taodan Granules in an Imiquimod-Induced Psoriasis-like Mouse Model
title_full Transcriptomic Analysis of the Mechanisms for Alleviating Psoriatic Dermatitis Using Taodan Granules in an Imiquimod-Induced Psoriasis-like Mouse Model
title_fullStr Transcriptomic Analysis of the Mechanisms for Alleviating Psoriatic Dermatitis Using Taodan Granules in an Imiquimod-Induced Psoriasis-like Mouse Model
title_full_unstemmed Transcriptomic Analysis of the Mechanisms for Alleviating Psoriatic Dermatitis Using Taodan Granules in an Imiquimod-Induced Psoriasis-like Mouse Model
title_short Transcriptomic Analysis of the Mechanisms for Alleviating Psoriatic Dermatitis Using Taodan Granules in an Imiquimod-Induced Psoriasis-like Mouse Model
title_sort transcriptomic analysis of the mechanisms for alleviating psoriatic dermatitis using taodan granules in an imiquimod-induced psoriasis-like mouse model
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114823/
https://www.ncbi.nlm.nih.gov/pubmed/33995034
http://dx.doi.org/10.3389/fphar.2021.632414
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