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The Effect of Inclusion Criteria on the Functional Properties Reported in Mouse Visual Cortex
Neurophysiology studies require the use of inclusion criteria to identify neurons responsive to the experimental stimuli. Five recent studies used calcium imaging to measure the preferred tuning properties of layer 2/3 pyramidal neurons in mouse visual areas. These five studies employed different in...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Society for Neuroscience
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114876/ https://www.ncbi.nlm.nih.gov/pubmed/33509948 http://dx.doi.org/10.1523/ENEURO.0188-20.2021 |
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author | Mesa, Natalia Waters, Jack de Vries, Saskia E. J. |
author_facet | Mesa, Natalia Waters, Jack de Vries, Saskia E. J. |
author_sort | Mesa, Natalia |
collection | PubMed |
description | Neurophysiology studies require the use of inclusion criteria to identify neurons responsive to the experimental stimuli. Five recent studies used calcium imaging to measure the preferred tuning properties of layer 2/3 pyramidal neurons in mouse visual areas. These five studies employed different inclusion criteria and reported different, sometimes conflicting results. Here, we examine how different inclusion criteria can impact reported tuning properties, modifying inclusion criteria to select different subpopulations from the same dataset of almost 17,000 layer 2/3 neurons from the Allen Brain Observatory. The choice of inclusion criteria greatly affected the mean tuning properties of the resulting subpopulations; indeed, the differences in mean tuning because of inclusion criteria were often of comparable magnitude to the differences between studies. In particular, the mean preferred temporal frequencies (TFs) of visual areas changed markedly with inclusion criteria, such that the rank ordering of visual areas based on their TF preferences changed with the percentage of neurons included. It has been suggested that differences in TF tuning support a hierarchy of mouse visual areas. These results demonstrate that our understanding of the functional organization of the mouse visual cortex obtained from previous experiments critically depends on the inclusion criteria used. |
format | Online Article Text |
id | pubmed-8114876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Society for Neuroscience |
record_format | MEDLINE/PubMed |
spelling | pubmed-81148762021-05-12 The Effect of Inclusion Criteria on the Functional Properties Reported in Mouse Visual Cortex Mesa, Natalia Waters, Jack de Vries, Saskia E. J. eNeuro Research Article: New Research Neurophysiology studies require the use of inclusion criteria to identify neurons responsive to the experimental stimuli. Five recent studies used calcium imaging to measure the preferred tuning properties of layer 2/3 pyramidal neurons in mouse visual areas. These five studies employed different inclusion criteria and reported different, sometimes conflicting results. Here, we examine how different inclusion criteria can impact reported tuning properties, modifying inclusion criteria to select different subpopulations from the same dataset of almost 17,000 layer 2/3 neurons from the Allen Brain Observatory. The choice of inclusion criteria greatly affected the mean tuning properties of the resulting subpopulations; indeed, the differences in mean tuning because of inclusion criteria were often of comparable magnitude to the differences between studies. In particular, the mean preferred temporal frequencies (TFs) of visual areas changed markedly with inclusion criteria, such that the rank ordering of visual areas based on their TF preferences changed with the percentage of neurons included. It has been suggested that differences in TF tuning support a hierarchy of mouse visual areas. These results demonstrate that our understanding of the functional organization of the mouse visual cortex obtained from previous experiments critically depends on the inclusion criteria used. Society for Neuroscience 2021-02-23 /pmc/articles/PMC8114876/ /pubmed/33509948 http://dx.doi.org/10.1523/ENEURO.0188-20.2021 Text en Copyright © 2021 Mesa et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article: New Research Mesa, Natalia Waters, Jack de Vries, Saskia E. J. The Effect of Inclusion Criteria on the Functional Properties Reported in Mouse Visual Cortex |
title | The Effect of Inclusion Criteria on the Functional Properties Reported in Mouse Visual Cortex |
title_full | The Effect of Inclusion Criteria on the Functional Properties Reported in Mouse Visual Cortex |
title_fullStr | The Effect of Inclusion Criteria on the Functional Properties Reported in Mouse Visual Cortex |
title_full_unstemmed | The Effect of Inclusion Criteria on the Functional Properties Reported in Mouse Visual Cortex |
title_short | The Effect of Inclusion Criteria on the Functional Properties Reported in Mouse Visual Cortex |
title_sort | effect of inclusion criteria on the functional properties reported in mouse visual cortex |
topic | Research Article: New Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114876/ https://www.ncbi.nlm.nih.gov/pubmed/33509948 http://dx.doi.org/10.1523/ENEURO.0188-20.2021 |
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