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Continuous Cultures of Plasmodium Falciparum Established in Tanzania from Patients with Acute Malaria

BACKGROUND: Malaria morbidity and mortality, almost entirely from Plasmodium falciparum, are still rampant in Africa: therefore, it is important to study the biology of the parasite and the parasite-host cell interactions. In vitro cultivation of Plasmodium falciparum is most useful for this purpose...

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Autores principales: Urio, Florence, Mkombachepa, Matilda, Rwegasira, Gration, Makene, Twilumba, Ngasala, Billy, Mselle, Teddy, Makani, Julie, Luzzatto, Lucio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Università Cattolica del Sacro Cuore 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114889/
https://www.ncbi.nlm.nih.gov/pubmed/34007424
http://dx.doi.org/10.4084/MJHID.2021.036
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author Urio, Florence
Mkombachepa, Matilda
Rwegasira, Gration
Makene, Twilumba
Ngasala, Billy
Mselle, Teddy
Makani, Julie
Luzzatto, Lucio
author_facet Urio, Florence
Mkombachepa, Matilda
Rwegasira, Gration
Makene, Twilumba
Ngasala, Billy
Mselle, Teddy
Makani, Julie
Luzzatto, Lucio
author_sort Urio, Florence
collection PubMed
description BACKGROUND: Malaria morbidity and mortality, almost entirely from Plasmodium falciparum, are still rampant in Africa: therefore, it is important to study the biology of the parasite and the parasite-host cell interactions. In vitro cultivation of Plasmodium falciparum is most useful for this purpose, as well as for investigating drug resistance and possible new therapies. Here we report that the Trager & Jensen continuous culture of P. falciparum can be established in a laboratory in Tanzania with minimal facilities and with modest expenditure. METHODOLOGY: This was an in-vitro set up of continuous culture of Plasmodium falciparum study, carried out in 2016–2020 at Muhimbili university of health and allied sciences, Dar-es salaam. Parasite samples were obtained from patients with acute malaria, frozen parasites, and live cultures. Data was collected and analyzed using GraphPad Prism version 8. RESULTS: We have successfully achieved exponential growth of existing strains that are used worldwide, as well as of parasites in clinical samples from patients with acute malaria. In the aim to optimize growth we have compared human serum and bovine serum albumin as components of the culture media. Additionally, culture synchronization has been achieved using sorbitol. CONCLUSION: This experimental system is now available to our institution and to researchers aiming at investigating drug sensitivity and mechanisms of protection against Plasmodium falciparum that accrue from various genes expressed in red cells.
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spelling pubmed-81148892021-05-17 Continuous Cultures of Plasmodium Falciparum Established in Tanzania from Patients with Acute Malaria Urio, Florence Mkombachepa, Matilda Rwegasira, Gration Makene, Twilumba Ngasala, Billy Mselle, Teddy Makani, Julie Luzzatto, Lucio Mediterr J Hematol Infect Dis Original Article BACKGROUND: Malaria morbidity and mortality, almost entirely from Plasmodium falciparum, are still rampant in Africa: therefore, it is important to study the biology of the parasite and the parasite-host cell interactions. In vitro cultivation of Plasmodium falciparum is most useful for this purpose, as well as for investigating drug resistance and possible new therapies. Here we report that the Trager & Jensen continuous culture of P. falciparum can be established in a laboratory in Tanzania with minimal facilities and with modest expenditure. METHODOLOGY: This was an in-vitro set up of continuous culture of Plasmodium falciparum study, carried out in 2016–2020 at Muhimbili university of health and allied sciences, Dar-es salaam. Parasite samples were obtained from patients with acute malaria, frozen parasites, and live cultures. Data was collected and analyzed using GraphPad Prism version 8. RESULTS: We have successfully achieved exponential growth of existing strains that are used worldwide, as well as of parasites in clinical samples from patients with acute malaria. In the aim to optimize growth we have compared human serum and bovine serum albumin as components of the culture media. Additionally, culture synchronization has been achieved using sorbitol. CONCLUSION: This experimental system is now available to our institution and to researchers aiming at investigating drug sensitivity and mechanisms of protection against Plasmodium falciparum that accrue from various genes expressed in red cells. Università Cattolica del Sacro Cuore 2021-05-01 /pmc/articles/PMC8114889/ /pubmed/34007424 http://dx.doi.org/10.4084/MJHID.2021.036 Text en https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Urio, Florence
Mkombachepa, Matilda
Rwegasira, Gration
Makene, Twilumba
Ngasala, Billy
Mselle, Teddy
Makani, Julie
Luzzatto, Lucio
Continuous Cultures of Plasmodium Falciparum Established in Tanzania from Patients with Acute Malaria
title Continuous Cultures of Plasmodium Falciparum Established in Tanzania from Patients with Acute Malaria
title_full Continuous Cultures of Plasmodium Falciparum Established in Tanzania from Patients with Acute Malaria
title_fullStr Continuous Cultures of Plasmodium Falciparum Established in Tanzania from Patients with Acute Malaria
title_full_unstemmed Continuous Cultures of Plasmodium Falciparum Established in Tanzania from Patients with Acute Malaria
title_short Continuous Cultures of Plasmodium Falciparum Established in Tanzania from Patients with Acute Malaria
title_sort continuous cultures of plasmodium falciparum established in tanzania from patients with acute malaria
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8114889/
https://www.ncbi.nlm.nih.gov/pubmed/34007424
http://dx.doi.org/10.4084/MJHID.2021.036
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