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Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort
MicroRNA (miRNA) circulating in plasma have been proposed as biomarkers for a variety of conditions and diseases, including complications during pregnancy. During pregnancy, about 15–25% of maternal plasma exosomes, a small size-class of EVs, are hypothesized to originate in the placenta, and may pl...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115775/ https://www.ncbi.nlm.nih.gov/pubmed/33979365 http://dx.doi.org/10.1371/journal.pone.0251259 |
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author | Foley, Helen Bermudez Howe, Caitlin G. Eckel, Sandrah P. Chavez, Thomas Gevorkian, Lili Reyes, Eileen Granada Kapanke, Bethany Martinez, Danilo Xue, Shanyan Suglia, Shakira F. Bastain, Theresa M. Marsit, Carmen Breton, Carrie V. |
author_facet | Foley, Helen Bermudez Howe, Caitlin G. Eckel, Sandrah P. Chavez, Thomas Gevorkian, Lili Reyes, Eileen Granada Kapanke, Bethany Martinez, Danilo Xue, Shanyan Suglia, Shakira F. Bastain, Theresa M. Marsit, Carmen Breton, Carrie V. |
author_sort | Foley, Helen Bermudez |
collection | PubMed |
description | MicroRNA (miRNA) circulating in plasma have been proposed as biomarkers for a variety of conditions and diseases, including complications during pregnancy. During pregnancy, about 15–25% of maternal plasma exosomes, a small size-class of EVs, are hypothesized to originate in the placenta, and may play a role in communication between the fetus and mother. However, few studies have addressed changes in miRNA over the course of pregnancy with repeated measures, nor focused on diverse populations. We describe changes in miRNA in early and late pregnancy from the MADRES cohort of primarily low-income Hispanic women based in Los Angeles, CA. miRNA derived from extracellular-vesicles (EVs) were isolated from maternal blood plasma samples collected in early and late pregnancy. In this study, we identified 64 of 130 detectable miRNA which significantly increased with gestational age at the time of collection (GA), and 26 which decreased with GA. Possible fetal sex-specific associations were observed for 30 of these 90 significant miRNA. Predicted gene targets for miRNA significantly associated with GA were identified using MirDIP and were found to be enriched for Gene Ontology categories that included energetic and metabolic processes but were underrepresented in immune-related categories. Circulating EV-associated miRNA during pregnancy are likely important for maternal-fetal communication, and may play roles in supporting and maintaining a healthy pregnancy, given the changing needs of the fetus. |
format | Online Article Text |
id | pubmed-8115775 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-81157752021-05-24 Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort Foley, Helen Bermudez Howe, Caitlin G. Eckel, Sandrah P. Chavez, Thomas Gevorkian, Lili Reyes, Eileen Granada Kapanke, Bethany Martinez, Danilo Xue, Shanyan Suglia, Shakira F. Bastain, Theresa M. Marsit, Carmen Breton, Carrie V. PLoS One Research Article MicroRNA (miRNA) circulating in plasma have been proposed as biomarkers for a variety of conditions and diseases, including complications during pregnancy. During pregnancy, about 15–25% of maternal plasma exosomes, a small size-class of EVs, are hypothesized to originate in the placenta, and may play a role in communication between the fetus and mother. However, few studies have addressed changes in miRNA over the course of pregnancy with repeated measures, nor focused on diverse populations. We describe changes in miRNA in early and late pregnancy from the MADRES cohort of primarily low-income Hispanic women based in Los Angeles, CA. miRNA derived from extracellular-vesicles (EVs) were isolated from maternal blood plasma samples collected in early and late pregnancy. In this study, we identified 64 of 130 detectable miRNA which significantly increased with gestational age at the time of collection (GA), and 26 which decreased with GA. Possible fetal sex-specific associations were observed for 30 of these 90 significant miRNA. Predicted gene targets for miRNA significantly associated with GA were identified using MirDIP and were found to be enriched for Gene Ontology categories that included energetic and metabolic processes but were underrepresented in immune-related categories. Circulating EV-associated miRNA during pregnancy are likely important for maternal-fetal communication, and may play roles in supporting and maintaining a healthy pregnancy, given the changing needs of the fetus. Public Library of Science 2021-05-12 /pmc/articles/PMC8115775/ /pubmed/33979365 http://dx.doi.org/10.1371/journal.pone.0251259 Text en © 2021 Foley et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Foley, Helen Bermudez Howe, Caitlin G. Eckel, Sandrah P. Chavez, Thomas Gevorkian, Lili Reyes, Eileen Granada Kapanke, Bethany Martinez, Danilo Xue, Shanyan Suglia, Shakira F. Bastain, Theresa M. Marsit, Carmen Breton, Carrie V. Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort |
title | Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort |
title_full | Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort |
title_fullStr | Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort |
title_full_unstemmed | Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort |
title_short | Extracellular vesicle-enriched miRNA profiles across pregnancy in the MADRES cohort |
title_sort | extracellular vesicle-enriched mirna profiles across pregnancy in the madres cohort |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115775/ https://www.ncbi.nlm.nih.gov/pubmed/33979365 http://dx.doi.org/10.1371/journal.pone.0251259 |
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