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Plasmids shape the diverse accessory resistomes of Escherichia coli ST131

The human gut microbiome includes beneficial, commensal and pathogenic bacteria that possess antimicrobial resistance (AMR) genes and exchange these predominantly through conjugative plasmids. Escherichia coli is a significant component of the gastrointestinal microbiome and is typically non-pathoge...

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Autores principales: Decano, Arun Gonzales, Tran, Nghia, Al-Foori, Hawriya, Al-Awadi, Buthaina, Campbell, Leigh, Ellison, Kevin, Mirabueno, Louisse Paolo, Nelson, Maddy, Power, Shane, Smith, Genevieve, Smyth, Cian, Vance, Zoe, Woods, Caitriona, Rahm, Alexander, Downing, Tim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115979/
https://www.ncbi.nlm.nih.gov/pubmed/33997610
http://dx.doi.org/10.1099/acmi.0.000179
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author Decano, Arun Gonzales
Tran, Nghia
Al-Foori, Hawriya
Al-Awadi, Buthaina
Campbell, Leigh
Ellison, Kevin
Mirabueno, Louisse Paolo
Nelson, Maddy
Power, Shane
Smith, Genevieve
Smyth, Cian
Vance, Zoe
Woods, Caitriona
Rahm, Alexander
Downing, Tim
author_facet Decano, Arun Gonzales
Tran, Nghia
Al-Foori, Hawriya
Al-Awadi, Buthaina
Campbell, Leigh
Ellison, Kevin
Mirabueno, Louisse Paolo
Nelson, Maddy
Power, Shane
Smith, Genevieve
Smyth, Cian
Vance, Zoe
Woods, Caitriona
Rahm, Alexander
Downing, Tim
author_sort Decano, Arun Gonzales
collection PubMed
description The human gut microbiome includes beneficial, commensal and pathogenic bacteria that possess antimicrobial resistance (AMR) genes and exchange these predominantly through conjugative plasmids. Escherichia coli is a significant component of the gastrointestinal microbiome and is typically non-pathogenic in this niche. In contrast, extra-intestinal pathogenic E. coli (ExPEC) including ST131 may occupy other environments like the urinary tract or bloodstream where they express genes enabling AMR and host cell adhesion like type 1 fimbriae. The extent to which commensal E. coli and uropathogenic ExPEC ST131 share AMR genes remains understudied at a genomic level, and we examined this here using a preterm infant resistome. We found that individual ST131 had small differences in AMR gene content relative to a larger shared resistome. Comparisons with a range of plasmids common in ST131 showed that AMR gene composition was driven by conjugation, recombination and mobile genetic elements. Plasmid pEK499 had extended regions in most ST131 Clade C isolates, and it had evidence of a co-evolutionary signal based on protein-level interactions with chromosomal gene products, as did pEK204 that had a type IV fimbrial pil operon. ST131 possessed extensive diversity of selective type 1, type IV, P and F17-like fimbriae genes that was highest in subclade C2. The structure and composition of AMR genes, plasmids and fimbriae vary widely in ST131 Clade C and this may mediate pathogenicity and infection outcomes.
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spelling pubmed-81159792021-05-13 Plasmids shape the diverse accessory resistomes of Escherichia coli ST131 Decano, Arun Gonzales Tran, Nghia Al-Foori, Hawriya Al-Awadi, Buthaina Campbell, Leigh Ellison, Kevin Mirabueno, Louisse Paolo Nelson, Maddy Power, Shane Smith, Genevieve Smyth, Cian Vance, Zoe Woods, Caitriona Rahm, Alexander Downing, Tim Access Microbiol Research Article The human gut microbiome includes beneficial, commensal and pathogenic bacteria that possess antimicrobial resistance (AMR) genes and exchange these predominantly through conjugative plasmids. Escherichia coli is a significant component of the gastrointestinal microbiome and is typically non-pathogenic in this niche. In contrast, extra-intestinal pathogenic E. coli (ExPEC) including ST131 may occupy other environments like the urinary tract or bloodstream where they express genes enabling AMR and host cell adhesion like type 1 fimbriae. The extent to which commensal E. coli and uropathogenic ExPEC ST131 share AMR genes remains understudied at a genomic level, and we examined this here using a preterm infant resistome. We found that individual ST131 had small differences in AMR gene content relative to a larger shared resistome. Comparisons with a range of plasmids common in ST131 showed that AMR gene composition was driven by conjugation, recombination and mobile genetic elements. Plasmid pEK499 had extended regions in most ST131 Clade C isolates, and it had evidence of a co-evolutionary signal based on protein-level interactions with chromosomal gene products, as did pEK204 that had a type IV fimbrial pil operon. ST131 possessed extensive diversity of selective type 1, type IV, P and F17-like fimbriae genes that was highest in subclade C2. The structure and composition of AMR genes, plasmids and fimbriae vary widely in ST131 Clade C and this may mediate pathogenicity and infection outcomes. Microbiology Society 2020-11-18 /pmc/articles/PMC8115979/ /pubmed/33997610 http://dx.doi.org/10.1099/acmi.0.000179 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
spellingShingle Research Article
Decano, Arun Gonzales
Tran, Nghia
Al-Foori, Hawriya
Al-Awadi, Buthaina
Campbell, Leigh
Ellison, Kevin
Mirabueno, Louisse Paolo
Nelson, Maddy
Power, Shane
Smith, Genevieve
Smyth, Cian
Vance, Zoe
Woods, Caitriona
Rahm, Alexander
Downing, Tim
Plasmids shape the diverse accessory resistomes of Escherichia coli ST131
title Plasmids shape the diverse accessory resistomes of Escherichia coli ST131
title_full Plasmids shape the diverse accessory resistomes of Escherichia coli ST131
title_fullStr Plasmids shape the diverse accessory resistomes of Escherichia coli ST131
title_full_unstemmed Plasmids shape the diverse accessory resistomes of Escherichia coli ST131
title_short Plasmids shape the diverse accessory resistomes of Escherichia coli ST131
title_sort plasmids shape the diverse accessory resistomes of escherichia coli st131
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8115979/
https://www.ncbi.nlm.nih.gov/pubmed/33997610
http://dx.doi.org/10.1099/acmi.0.000179
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